Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex

• Autor(a) principal: Nesic, Maja D.
• Data de Publicação: 2022
• Outros Autores: Ducic, Tanja, Gonçalves, Mara, Stepić, Milutin, Algarra, Manuel, Soto, Juan, Gemović, Branislava, Bandosz, Teresa J., Petkovic, Marijana
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.13/5234
• topic: Anticancer photodynamic therapy; Biochemical changes; N-doped carbon dots; Carbon dots; Ru-metallodrug; .; Faculdade de Ciências Exatas e da Engenharia

Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.