Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/47101 |
Resumo: | © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Cutaneous secretions of amphibians have bioactive compounds, such as peptides, with potential for biotechnological applications. Therefore, this study aimed to determine the primary structure and investigate peptides obtained from the cutaneous secretions of the amphibian, Leptodactylus vastus, as a source of bioactive molecules. The peptides obtained possessed the amino acid sequences, GVVDILKGAAKDLAGH and GVVDILKGAAKDLAGHLASKV, with monoisotopic masses of [M + H]± = 1563.8 Da and [M + H]± = 2062.4 Da, respectively. The molecules were characterized as peptides of the class of ocellatins and were named as Ocellatin-K1(1-16) and Ocellatin-K1(1-21). Functional analysis revealed that Ocellatin-K1(1-16) and Ocellatin-K1(1-21) showed weak antibacterial activity. However, treatment of mice with these ocellatins reduced the nitrite and malondialdehyde content. Moreover, superoxide dismutase enzymatic activity and glutathione concentration were increased in the hippocampus of mice. In addition, Ocellatin-K1(1-16) and Ocellatin-K1(1-21) were effective in impairing lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) formation and NF-kB activation in living microglia. We incubated hippocampal neurons with microglial conditioned media treated with LPS and LPS in the presence of Ocellatin-K1(1-16) and Ocellatin-K1(1-21) and observed that both peptides reduced the oxidative stress in hippocampal neurons. Furthermore, these ocellatins demonstrated low cytotoxicity towards erythrocytes. These functional properties suggest possible to neuromodulatory therapeutic applications.Alexandra Plácido is a recipient of a post-doctoral grant from the project FCT (PTDC/BII-BIO/31158/2017). Renato Socodato and Camila Cabral Portugal hold postdoctoral fellowships from FCT (Refs: SFRH/BPD/91833/2012 and FRH/BPD/91962/2012, respectively). This work was funded through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE) with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT 2020Springer NatureRepositório da Universidade de LisboaSousa, Nayara A.Oliveira, Guilherme A. L.de Oliveira, Ana PatríciaLopes, André Luís F.Iles, BrunoNogueira, Kerolayne M.Araújo, Thiago S. L.Souza, Luan K. M.Araújo, Alyne R.Ramos-Jesus, JoilsonPlácido, AlexandraAmaral, Constança Pais DoCampelo, Yuri D. M.Barbosa, Eder AlvesPortugal, Camila C.Socodato, RenatoLobo, AndreaRelvas, JoaoBemquerer, MarceloEaton, PeterLeite, José Roberto S. A.Medeiros, Jand Venes R.2021-03-26T15:52:52Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/47101engSci Rep. 2020 Feb 14;10(1):269610.1038/s41598-020-59665-12045-2322info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:49:45Zoai:repositorio.ul.pt:10451/47101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:59:10.804451Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
title |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
spellingShingle |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons Sousa, Nayara A. |
title_short |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
title_full |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
title_fullStr |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
title_full_unstemmed |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
title_sort |
Novel ocellatin peptides mitigate LPS-induced ROS formation and NF-kB activation in microglia and hippocampal neurons |
author |
Sousa, Nayara A. |
author_facet |
Sousa, Nayara A. Oliveira, Guilherme A. L. de Oliveira, Ana Patrícia Lopes, André Luís F. Iles, Bruno Nogueira, Kerolayne M. Araújo, Thiago S. L. Souza, Luan K. M. Araújo, Alyne R. Ramos-Jesus, Joilson Plácido, Alexandra Amaral, Constança Pais Do Campelo, Yuri D. M. Barbosa, Eder Alves Portugal, Camila C. Socodato, Renato Lobo, Andrea Relvas, Joao Bemquerer, Marcelo Eaton, Peter Leite, José Roberto S. A. Medeiros, Jand Venes R. |
author_role |
author |
author2 |
Oliveira, Guilherme A. L. de Oliveira, Ana Patrícia Lopes, André Luís F. Iles, Bruno Nogueira, Kerolayne M. Araújo, Thiago S. L. Souza, Luan K. M. Araújo, Alyne R. Ramos-Jesus, Joilson Plácido, Alexandra Amaral, Constança Pais Do Campelo, Yuri D. M. Barbosa, Eder Alves Portugal, Camila C. Socodato, Renato Lobo, Andrea Relvas, Joao Bemquerer, Marcelo Eaton, Peter Leite, José Roberto S. A. Medeiros, Jand Venes R. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Sousa, Nayara A. Oliveira, Guilherme A. L. de Oliveira, Ana Patrícia Lopes, André Luís F. Iles, Bruno Nogueira, Kerolayne M. Araújo, Thiago S. L. Souza, Luan K. M. Araújo, Alyne R. Ramos-Jesus, Joilson Plácido, Alexandra Amaral, Constança Pais Do Campelo, Yuri D. M. Barbosa, Eder Alves Portugal, Camila C. Socodato, Renato Lobo, Andrea Relvas, Joao Bemquerer, Marcelo Eaton, Peter Leite, José Roberto S. A. Medeiros, Jand Venes R. |
description |
© The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-03-26T15:52:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/47101 |
url |
http://hdl.handle.net/10451/47101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sci Rep. 2020 Feb 14;10(1):2696 10.1038/s41598-020-59665-1 2045-2322 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Springer Nature |
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Springer Nature |
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