Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK

Detalhes bibliográficos
Autor(a) principal: ZHOU,QIN
Data de Publicação: 2020
Outros Autores: GUO,DONGKAI, LI,XINJIA, WANG,YIXUAN, YE,XIAOLI, XUE,SUDONG, WANG,XIAOYU
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700807
Resumo: Abstract Microglia are the resident immune cells in the central nervous system (CNS), which play important roles in the repair of neuroinflammatory injury. The present study investigated the anti-neuroinflammatory effects of vinpocetine induced by lipopolysaccharide (LPS) in BV2 microglia. BV2 microglia were pretreated with vinpocetine, and then stimulated with LPS (100 ng/mL). The cytotoxicity of BV2 microglia was assessed by MTT assay. The expression levels of nitrite oxide were measured by Griess assay. Proinflammatory cytokines and mediators were determined by Western blot, ELISA, or quantitative real-time PCR. Vinpocetine significantly decreased the generation of nitric oxide-inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2 in a dose-dependent manner. In addition, vinpocetine decreased the production of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-1β. Furthermore, it was observed that phosphorylation levels of AMPK (Thr-172) decreased in LPS-stimulated BV2 microglia. Vinpocetine treatment increased AMPK phosphorylation in LPS-stimulated BV2 microglia. AMPK inhibition by siRNA blocked the anti-inflammatory effects of vinpocetine induced by LPS in BV2 microglia. The overall results demonstrate that vinpocetine has anti-inflammatory effects on LPS-stimulated BV2 microglia via inducing phosphorylation of AMPK, suggesting that vinpocetine is a potential therapeutic agent in neuroinflammatory injury.
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spelling Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPKVinpocetineBV2 microgliaLPSneuroinflammationAMPKAbstract Microglia are the resident immune cells in the central nervous system (CNS), which play important roles in the repair of neuroinflammatory injury. The present study investigated the anti-neuroinflammatory effects of vinpocetine induced by lipopolysaccharide (LPS) in BV2 microglia. BV2 microglia were pretreated with vinpocetine, and then stimulated with LPS (100 ng/mL). The cytotoxicity of BV2 microglia was assessed by MTT assay. The expression levels of nitrite oxide were measured by Griess assay. Proinflammatory cytokines and mediators were determined by Western blot, ELISA, or quantitative real-time PCR. Vinpocetine significantly decreased the generation of nitric oxide-inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2 in a dose-dependent manner. In addition, vinpocetine decreased the production of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-1β. Furthermore, it was observed that phosphorylation levels of AMPK (Thr-172) decreased in LPS-stimulated BV2 microglia. Vinpocetine treatment increased AMPK phosphorylation in LPS-stimulated BV2 microglia. AMPK inhibition by siRNA blocked the anti-inflammatory effects of vinpocetine induced by LPS in BV2 microglia. The overall results demonstrate that vinpocetine has anti-inflammatory effects on LPS-stimulated BV2 microglia via inducing phosphorylation of AMPK, suggesting that vinpocetine is a potential therapeutic agent in neuroinflammatory injury.Academia Brasileira de Ciências2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700807Anais da Academia Brasileira de Ciências v.92 n.4 2020reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765202020200241info:eu-repo/semantics/openAccessZHOU,QINGUO,DONGKAILI,XINJIAWANG,YIXUANYE,XIAOLIXUE,SUDONGWANG,XIAOYUeng2020-11-18T00:00:00Zoai:scielo:S0001-37652020000700807Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2020-11-18T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
title Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
spellingShingle Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
ZHOU,QIN
Vinpocetine
BV2 microglia
LPS
neuroinflammation
AMPK
title_short Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
title_full Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
title_fullStr Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
title_full_unstemmed Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
title_sort Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK
author ZHOU,QIN
author_facet ZHOU,QIN
GUO,DONGKAI
LI,XINJIA
WANG,YIXUAN
YE,XIAOLI
XUE,SUDONG
WANG,XIAOYU
author_role author
author2 GUO,DONGKAI
LI,XINJIA
WANG,YIXUAN
YE,XIAOLI
XUE,SUDONG
WANG,XIAOYU
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv ZHOU,QIN
GUO,DONGKAI
LI,XINJIA
WANG,YIXUAN
YE,XIAOLI
XUE,SUDONG
WANG,XIAOYU
dc.subject.por.fl_str_mv Vinpocetine
BV2 microglia
LPS
neuroinflammation
AMPK
topic Vinpocetine
BV2 microglia
LPS
neuroinflammation
AMPK
description Abstract Microglia are the resident immune cells in the central nervous system (CNS), which play important roles in the repair of neuroinflammatory injury. The present study investigated the anti-neuroinflammatory effects of vinpocetine induced by lipopolysaccharide (LPS) in BV2 microglia. BV2 microglia were pretreated with vinpocetine, and then stimulated with LPS (100 ng/mL). The cytotoxicity of BV2 microglia was assessed by MTT assay. The expression levels of nitrite oxide were measured by Griess assay. Proinflammatory cytokines and mediators were determined by Western blot, ELISA, or quantitative real-time PCR. Vinpocetine significantly decreased the generation of nitric oxide-inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2 in a dose-dependent manner. In addition, vinpocetine decreased the production of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-1β. Furthermore, it was observed that phosphorylation levels of AMPK (Thr-172) decreased in LPS-stimulated BV2 microglia. Vinpocetine treatment increased AMPK phosphorylation in LPS-stimulated BV2 microglia. AMPK inhibition by siRNA blocked the anti-inflammatory effects of vinpocetine induced by LPS in BV2 microglia. The overall results demonstrate that vinpocetine has anti-inflammatory effects on LPS-stimulated BV2 microglia via inducing phosphorylation of AMPK, suggesting that vinpocetine is a potential therapeutic agent in neuroinflammatory injury.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700807
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000700807
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765202020200241
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.92 n.4 2020
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
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institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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