An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108044 https://doi.org/10.1038/s41598-018-29662-6 |
Resumo: | The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87-1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction. |
id |
RCAP_120a19d4f62c3883d65c5558f5afa44c |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/108044 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicideAnimalsArthropodsEnvironmental PollutantsGene Expression RegulationGene OntologyGene Regulatory NetworksGenomicsHerbicidesRNA, MessengerReproductionSurvival AnalysisTranscriptomeToxicity TestsThe use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87-1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction.Springer Nature2018-07-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108044http://hdl.handle.net/10316/108044https://doi.org/10.1038/s41598-018-29662-6eng2045-2322Simões, TiagoNovais, Sara CLuz, Tiago Natal daDevreese, Bartde Boer, TjalfRoelofs, DickSousa, José P.van Straalen, Nico MLemos, Marco F. L.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-07T14:15:50Zoai:estudogeral.uc.pt:10316/108044Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:18.972Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
title |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
spellingShingle |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide Simões, Tiago Animals Arthropods Environmental Pollutants Gene Expression Regulation Gene Ontology Gene Regulatory Networks Genomics Herbicides RNA, Messenger Reproduction Survival Analysis Transcriptome Toxicity Tests |
title_short |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
title_full |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
title_fullStr |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
title_full_unstemmed |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
title_sort |
An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide |
author |
Simões, Tiago |
author_facet |
Simões, Tiago Novais, Sara C Luz, Tiago Natal da Devreese, Bart de Boer, Tjalf Roelofs, Dick Sousa, José P. van Straalen, Nico M Lemos, Marco F. L. |
author_role |
author |
author2 |
Novais, Sara C Luz, Tiago Natal da Devreese, Bart de Boer, Tjalf Roelofs, Dick Sousa, José P. van Straalen, Nico M Lemos, Marco F. L. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Simões, Tiago Novais, Sara C Luz, Tiago Natal da Devreese, Bart de Boer, Tjalf Roelofs, Dick Sousa, José P. van Straalen, Nico M Lemos, Marco F. L. |
dc.subject.por.fl_str_mv |
Animals Arthropods Environmental Pollutants Gene Expression Regulation Gene Ontology Gene Regulatory Networks Genomics Herbicides RNA, Messenger Reproduction Survival Analysis Transcriptome Toxicity Tests |
topic |
Animals Arthropods Environmental Pollutants Gene Expression Regulation Gene Ontology Gene Regulatory Networks Genomics Herbicides RNA, Messenger Reproduction Survival Analysis Transcriptome Toxicity Tests |
description |
The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87-1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108044 http://hdl.handle.net/10316/108044 https://doi.org/10.1038/s41598-018-29662-6 |
url |
http://hdl.handle.net/10316/108044 https://doi.org/10.1038/s41598-018-29662-6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799134128473374720 |