Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action

Detalhes bibliográficos
Autor(a) principal: Anjos, MNV
Data de Publicação: 2020
Outros Autores: Araújo-Neto, LN, Buonafina, MDS, Neves, RP, Souza, ER, Bezerra, ICF, Ferreira, MRA, Soares, LAL, Coutinho, HDM, Martins, N, Silva, MV, Correia, MTdS
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/143527
Resumo: Background: Opportunistic fungal infections are increasingly common, with Candida albicans being the most common etiological agent; however, in recent years, episodes of candidiasis caused by non-albicans Candida species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti-Candida activity of Ocotea glomerata extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed. Methods: Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests. Results: All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 µg/mL) on C. krusei. The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for C. krusei and C. albicans, respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against C. krusei. Methanol extract did not reveal effects by ergosterol and sorbitol assays; however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death. Conclusion: O. glomerata methanol extract may be viewed as a rich source of biomolecules with antifungal activity against Candida spp.
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spelling Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of actionAnti-candida activityMechanism of actionNatural productsOcotea glomerataBackground: Opportunistic fungal infections are increasingly common, with Candida albicans being the most common etiological agent; however, in recent years, episodes of candidiasis caused by non-albicans Candida species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti-Candida activity of Ocotea glomerata extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed. Methods: Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests. Results: All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 µg/mL) on C. krusei. The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for C. krusei and C. albicans, respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against C. krusei. Methanol extract did not reveal effects by ergosterol and sorbitol assays; however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death. Conclusion: O. glomerata methanol extract may be viewed as a rich source of biomolecules with antifungal activity against Candida spp.MDPI20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/143527eng2079-638210.3390/antibiotics9070394Anjos, MNVAraújo-Neto, LNBuonafina, MDSNeves, RPSouza, ERBezerra, ICFFerreira, MRASoares, LALCoutinho, HDMMartins, NSilva, MVCorreia, MTdSinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:41:36Zoai:repositorio-aberto.up.pt:10216/143527Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:29:51.157857Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
title Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
spellingShingle Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
Anjos, MNV
Anti-candida activity
Mechanism of action
Natural products
Ocotea glomerata
title_short Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
title_full Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
title_fullStr Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
title_full_unstemmed Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
title_sort Ocotea glomerata (Nees) mez extract and fractions: Chemical characterization, anti-Candida activity and related mechanism of action
author Anjos, MNV
author_facet Anjos, MNV
Araújo-Neto, LN
Buonafina, MDS
Neves, RP
Souza, ER
Bezerra, ICF
Ferreira, MRA
Soares, LAL
Coutinho, HDM
Martins, N
Silva, MV
Correia, MTdS
author_role author
author2 Araújo-Neto, LN
Buonafina, MDS
Neves, RP
Souza, ER
Bezerra, ICF
Ferreira, MRA
Soares, LAL
Coutinho, HDM
Martins, N
Silva, MV
Correia, MTdS
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Anjos, MNV
Araújo-Neto, LN
Buonafina, MDS
Neves, RP
Souza, ER
Bezerra, ICF
Ferreira, MRA
Soares, LAL
Coutinho, HDM
Martins, N
Silva, MV
Correia, MTdS
dc.subject.por.fl_str_mv Anti-candida activity
Mechanism of action
Natural products
Ocotea glomerata
topic Anti-candida activity
Mechanism of action
Natural products
Ocotea glomerata
description Background: Opportunistic fungal infections are increasingly common, with Candida albicans being the most common etiological agent; however, in recent years, episodes of candidiasis caused by non-albicans Candida species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti-Candida activity of Ocotea glomerata extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed. Methods: Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests. Results: All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 µg/mL) on C. krusei. The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for C. krusei and C. albicans, respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against C. krusei. Methanol extract did not reveal effects by ergosterol and sorbitol assays; however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death. Conclusion: O. glomerata methanol extract may be viewed as a rich source of biomolecules with antifungal activity against Candida spp.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/143527
url https://hdl.handle.net/10216/143527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-6382
10.3390/antibiotics9070394
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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