Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1948 |
Resumo: | Aims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. Conclusions: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. Significance of and Impact of the Study: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed. |
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Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formationAcrylicHaemagglutinationMedical devicesNosocomial infectionsPNAGStaphylococcus epidermidisScience & TechnologyAims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. Conclusions: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. Significance of and Impact of the Study: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.NIH - grant AI 46706.Fundação para a Ciência e a Tecnologia (FCT) – Programa Operacional “Ciência, Tecnologia, Inovação” (POCTI) - POCTI/ESP/42688/2001, SFRH/BD/8676/2002.Society for Applied MicrobiologyUniversidade do MinhoCerca, NunoPier, Gerald B.Vilanova, ManuelOliveira, RosárioAzeredo, Joana20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1948eng"Letters in applied microbiology". ISSN 0266-8254. 39 (2004) 420-424.0266-825410.1111/j.1472-765X.2004.01601.x15482432info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:24Zoai:repositorium.sdum.uminho.pt:1822/1948Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:04.398011Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
title |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
spellingShingle |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation Cerca, Nuno Acrylic Haemagglutination Medical devices Nosocomial infections PNAG Staphylococcus epidermidis Science & Technology |
title_short |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
title_full |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
title_fullStr |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
title_full_unstemmed |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
title_sort |
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation |
author |
Cerca, Nuno |
author_facet |
Cerca, Nuno Pier, Gerald B. Vilanova, Manuel Oliveira, Rosário Azeredo, Joana |
author_role |
author |
author2 |
Pier, Gerald B. Vilanova, Manuel Oliveira, Rosário Azeredo, Joana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Cerca, Nuno Pier, Gerald B. Vilanova, Manuel Oliveira, Rosário Azeredo, Joana |
dc.subject.por.fl_str_mv |
Acrylic Haemagglutination Medical devices Nosocomial infections PNAG Staphylococcus epidermidis Science & Technology |
topic |
Acrylic Haemagglutination Medical devices Nosocomial infections PNAG Staphylococcus epidermidis Science & Technology |
description |
Aims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. Conclusions: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. Significance of and Impact of the Study: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1948 |
url |
http://hdl.handle.net/1822/1948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Letters in applied microbiology". ISSN 0266-8254. 39 (2004) 420-424. 0266-8254 10.1111/j.1472-765X.2004.01601.x 15482432 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society for Applied Microbiology |
publisher.none.fl_str_mv |
Society for Applied Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132358824165376 |