Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area

Detalhes bibliográficos
Autor(a) principal: Cavadas, Joana
Data de Publicação: 2022
Outros Autores: Parreira, Ricardo, Leonardo, Inês, Barreto Crespo, Maria Teresa, Nunes, Mónica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147807
Resumo: Funding Information: This work received financial support from the AgriWWater Project (PTDC/CTA-AMB/29586/2017), iNOVA4Health (UID/Multi/04462/2019), and Global Health and Tropical Medicine Center (which is funded through FCT contract UID/Multi/04413/2013). Publisher Copyright: © 2022 by the authors.
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spelling Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Areaenvironmental watershuman adenovirusmolecular diversitynested PCRnext-generation sequencingphylogenetic analysiswastewaterQR MicrobiologyRA Public aspects of medicineMicrobiologyMicrobiology (medical)VirologySDG 3 - Good Health and Well-beingSDG 6 - Clean Water and SanitationSDG 11 - Sustainable Cities and CommunitiesFunding Information: This work received financial support from the AgriWWater Project (PTDC/CTA-AMB/29586/2017), iNOVA4Health (UID/Multi/04462/2019), and Global Health and Tropical Medicine Center (which is funded through FCT contract UID/Multi/04413/2013). Publisher Copyright: © 2022 by the authors.In face of the absence of epidemiological data regarding the circulation of human adenoviruses (HAdV) in Portugal, this study aimed at the evaluation of their molecular diversity in waste and environmental waters in the Lisbon Metropolitan Area (LMA). Using samples collected between 2018 and 2021, the HAdV hexon protein-coding sequence was partially amplified using three nested touch-down PCR protocols. The amplification products obtained were analyzed in parallel by two approaches: molecular cloning followed by Sanger sequencing and Next-Generation Sequencing (NGS) using Illumina® sequencing. The analysis of NGS-generated data allowed the identification of a higher diversity of HAdV-A (19%), -B (1%), -C (3%), -D (24%), and -F (25%) viral types, along with murine adenovirus (MAdV-2; 30%) in the wastewater treatment plant samples. On the other hand, HAdV-A (19%), -D (32%), and -F (36%) were identified in environmental samples, and possibly MAdV-2 (14%). These results demonstrate the presence of fecal contamination in environmental waters and the assessment of the diversity of this virus provides important information regarding the distribution of HAdV in LMA, including the detection of HAdV-F41, the most frequently reported in water worldwide.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Universidade NOVA de LisboaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCavadas, JoanaParreira, RicardoLeonardo, InêsBarreto Crespo, Maria TeresaNunes, Mónica2023-01-18T22:16:56Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147807eng2076-2607PURE: 49709411https://doi.org/10.3390/microorganisms10122443info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:03Zoai:run.unl.pt:10362/147807Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:05.194884Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
title Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
spellingShingle Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
Cavadas, Joana
environmental waters
human adenovirus
molecular diversity
nested PCR
next-generation sequencing
phylogenetic analysis
wastewater
QR Microbiology
RA Public aspects of medicine
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 6 - Clean Water and Sanitation
SDG 11 - Sustainable Cities and Communities
title_short Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
title_full Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
title_fullStr Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
title_full_unstemmed Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
title_sort Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
author Cavadas, Joana
author_facet Cavadas, Joana
Parreira, Ricardo
Leonardo, Inês
Barreto Crespo, Maria Teresa
Nunes, Mónica
author_role author
author2 Parreira, Ricardo
Leonardo, Inês
Barreto Crespo, Maria Teresa
Nunes, Mónica
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
Universidade NOVA de Lisboa
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Cavadas, Joana
Parreira, Ricardo
Leonardo, Inês
Barreto Crespo, Maria Teresa
Nunes, Mónica
dc.subject.por.fl_str_mv environmental waters
human adenovirus
molecular diversity
nested PCR
next-generation sequencing
phylogenetic analysis
wastewater
QR Microbiology
RA Public aspects of medicine
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 6 - Clean Water and Sanitation
SDG 11 - Sustainable Cities and Communities
topic environmental waters
human adenovirus
molecular diversity
nested PCR
next-generation sequencing
phylogenetic analysis
wastewater
QR Microbiology
RA Public aspects of medicine
Microbiology
Microbiology (medical)
Virology
SDG 3 - Good Health and Well-being
SDG 6 - Clean Water and Sanitation
SDG 11 - Sustainable Cities and Communities
description Funding Information: This work received financial support from the AgriWWater Project (PTDC/CTA-AMB/29586/2017), iNOVA4Health (UID/Multi/04462/2019), and Global Health and Tropical Medicine Center (which is funded through FCT contract UID/Multi/04413/2013). Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-01-18T22:16:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147807
url http://hdl.handle.net/10362/147807
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 49709411
https://doi.org/10.3390/microorganisms10122443
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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