Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1371/journal.ppat.1003720 |
Resumo: | This work was supported by PTDC/BIA-BCM/65872/2006 (www.fct.pt) and ERC-AJ208631 (erc.europa.eu) grants (AJ), ARC (www.arc-cancer.net) and FCT SFRH/BPD/44613/2008 (AZR), EMBO ALTF 178-2009 (www.embo.org) (JCR), and the Gulbenkian Institute PhD Program (www.igc.gulbenkian.pt) and FCT SFRH/BD/51175/2010 (ABL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in DrosophilaPEPTIDE GENE-EXPRESSIONAPOPTOTIC CELLSPSEUDOMONAS-AERUGINOSAHEMOCYTE MIGRATIONCELLULAR-IMMUNITYMUTANTS REVEALHOST-DEFENSEBLOOD-CELLSPHAGOCYTOSISRECEPTORParasitologyMicrobiologyImmunologyMolecular BiologyGeneticsVirologyThis work was supported by PTDC/BIA-BCM/65872/2006 (www.fct.pt) and ERC-AJ208631 (erc.europa.eu) grants (AJ), ARC (www.arc-cancer.net) and FCT SFRH/BPD/44613/2008 (AZR), EMBO ALTF 178-2009 (www.embo.org) (JCR), and the Gulbenkian Institute PhD Program (www.igc.gulbenkian.pt) and FCT SFRH/BD/51175/2010 (ABL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Coupling immunity and development is essential to ensure survival despite changing internal conditions in the organism. Drosophila metamorphosis represents a striking example of drastic and systemic physiological changes that need to be integrated with the innate immune system. However, nothing is known about the mechanisms that coordinate development and immune cell activity in the transition from larva to adult. Here, we reveal that regulation of macrophage-like cells (hemocytes) by the steroid hormone ecdysone is essential for an effective innate immune response over metamorphosis. Although it is generally accepted that steroid hormones impact immunity in mammals, their action on monocytes (e.g. macrophages and neutrophils) is still not well understood. Here in a simpler model system, we used an approach that allows in vivo, cell autonomous analysis of hormonal regulation of innate immune cells, by combining genetic manipulation with flow cytometry, high-resolution time-lapse imaging and tissue-specific transcriptomic analysis. We show that in response to ecdysone, hemocytes rapidly upregulate actin dynamics, motility and phagocytosis of apoptotic corpses, and acquire the ability to chemotax to damaged epithelia. Most importantly, individuals lacking ecdysone-activated hemocytes are defective in bacterial phagocytosis and are fatally susceptible to infection by bacteria ingested at larval stages, despite the normal systemic and local production of antimicrobial peptides. This decrease in survival is comparable to the one observed in pupae lacking immune cells altogether, indicating that ecdysone-regulation is essential for hemocyte immune functions and survival after infection. Microarray analysis of hemocytes revealed a large set of genes regulated at metamorphosis by EcR signaling, among which many are known to function in cell motility, cell shape or phagocytosis. This study demonstrates an important role for steroid hormone regulation of immunity in vivo in Drosophila, and paves the way for genetic dissection of the mechanisms at work behind steroid regulation of innate immune cells.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNRegan, Jennifer C.Brandão, Ana S.Leitão, Alexandre B.Mantas Dias, Ângela RaquelSucena, ÉlioJacinto, AntónioZaidman-Rémy, Anna2017-09-14T22:03:00Z2013-102013-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttps://doi.org/10.1371/journal.ppat.1003720eng1553-7366PURE: 3129368http://www.scopus.com/inward/record.url?scp=84887276025&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.ppat.1003720info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:11:29Zoai:run.unl.pt:10362/23259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:43.617636Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
title |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
spellingShingle |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila Regan, Jennifer C. PEPTIDE GENE-EXPRESSION APOPTOTIC CELLS PSEUDOMONAS-AERUGINOSA HEMOCYTE MIGRATION CELLULAR-IMMUNITY MUTANTS REVEAL HOST-DEFENSE BLOOD-CELLS PHAGOCYTOSIS RECEPTOR Parasitology Microbiology Immunology Molecular Biology Genetics Virology |
title_short |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
title_full |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
title_fullStr |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
title_full_unstemmed |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
title_sort |
Steroid Hormone Signaling Is Essential to Regulate Innate Immune Cells and Fight Bacterial Infection in Drosophila |
author |
Regan, Jennifer C. |
author_facet |
Regan, Jennifer C. Brandão, Ana S. Leitão, Alexandre B. Mantas Dias, Ângela Raquel Sucena, Élio Jacinto, António Zaidman-Rémy, Anna |
author_role |
author |
author2 |
Brandão, Ana S. Leitão, Alexandre B. Mantas Dias, Ângela Raquel Sucena, Élio Jacinto, António Zaidman-Rémy, Anna |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
dc.contributor.author.fl_str_mv |
Regan, Jennifer C. Brandão, Ana S. Leitão, Alexandre B. Mantas Dias, Ângela Raquel Sucena, Élio Jacinto, António Zaidman-Rémy, Anna |
dc.subject.por.fl_str_mv |
PEPTIDE GENE-EXPRESSION APOPTOTIC CELLS PSEUDOMONAS-AERUGINOSA HEMOCYTE MIGRATION CELLULAR-IMMUNITY MUTANTS REVEAL HOST-DEFENSE BLOOD-CELLS PHAGOCYTOSIS RECEPTOR Parasitology Microbiology Immunology Molecular Biology Genetics Virology |
topic |
PEPTIDE GENE-EXPRESSION APOPTOTIC CELLS PSEUDOMONAS-AERUGINOSA HEMOCYTE MIGRATION CELLULAR-IMMUNITY MUTANTS REVEAL HOST-DEFENSE BLOOD-CELLS PHAGOCYTOSIS RECEPTOR Parasitology Microbiology Immunology Molecular Biology Genetics Virology |
description |
This work was supported by PTDC/BIA-BCM/65872/2006 (www.fct.pt) and ERC-AJ208631 (erc.europa.eu) grants (AJ), ARC (www.arc-cancer.net) and FCT SFRH/BPD/44613/2008 (AZR), EMBO ALTF 178-2009 (www.embo.org) (JCR), and the Gulbenkian Institute PhD Program (www.igc.gulbenkian.pt) and FCT SFRH/BD/51175/2010 (ABL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10 2013-10-01T00:00:00Z 2017-09-14T22:03:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1371/journal.ppat.1003720 |
url |
https://doi.org/10.1371/journal.ppat.1003720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1553-7366 PURE: 3129368 http://www.scopus.com/inward/record.url?scp=84887276025&partnerID=8YFLogxK https://doi.org/10.1371/journal.ppat.1003720 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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