A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/42529 |
Resumo: | Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution. |
id |
RCAP_14337530100760feeae5ac76a9f0cb21 |
---|---|
oai_identifier_str |
oai:repositorio.ucp.pt:10400.14/42529 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosisCohesinCondensinMitotic transcriptionSNF2 familyTopoisomerase 2Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution.Veritati - Repositório Institucional da Universidade Católica PortuguesaCarmo, CatarinaCoelho, JoãoSilva, Rui D.Tavares, AlexandraBoavida, AnaGaetani, PaolaGuilgur, Leonardo G.Martinho, Rui GonçaloOliveira, Raquel A.2023-09-20T15:54:37Z2023-09-062023-09-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42529eng1469-221X10.15252/embr.2022564638516519995737462213001031927900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-26T01:44:15Zoai:repositorio.ucp.pt:10400.14/42529Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:30:58.546499Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
title |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
spellingShingle |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis Carmo, Catarina Cohesin Condensin Mitotic transcription SNF2 family Topoisomerase 2 |
title_short |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
title_full |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
title_fullStr |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
title_full_unstemmed |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
title_sort |
A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis |
author |
Carmo, Catarina |
author_facet |
Carmo, Catarina Coelho, João Silva, Rui D. Tavares, Alexandra Boavida, Ana Gaetani, Paola Guilgur, Leonardo G. Martinho, Rui Gonçalo Oliveira, Raquel A. |
author_role |
author |
author2 |
Coelho, João Silva, Rui D. Tavares, Alexandra Boavida, Ana Gaetani, Paola Guilgur, Leonardo G. Martinho, Rui Gonçalo Oliveira, Raquel A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Carmo, Catarina Coelho, João Silva, Rui D. Tavares, Alexandra Boavida, Ana Gaetani, Paola Guilgur, Leonardo G. Martinho, Rui Gonçalo Oliveira, Raquel A. |
dc.subject.por.fl_str_mv |
Cohesin Condensin Mitotic transcription SNF2 family Topoisomerase 2 |
topic |
Cohesin Condensin Mitotic transcription SNF2 family Topoisomerase 2 |
description |
Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-20T15:54:37Z 2023-09-06 2023-09-06T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/42529 |
url |
http://hdl.handle.net/10400.14/42529 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1469-221X 10.15252/embr.202256463 85165199957 37462213 001031927900001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133578450173952 |