Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation

Detalhes bibliográficos
Autor(a) principal: Piskadlo, Ewa
Data de Publicação: 2017
Outros Autores: Tavares, Alexandra, Oliveira, Raquel A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/826
Resumo: This deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://elifesciences.org/articles/26120/figures.
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spelling Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenationDrosophila melanogastercell biologychromosome condensationcondensinmitosistopoisomeraseThis deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://elifesciences.org/articles/26120/figures.Mitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of de novo Topoisomerase II (TopoII)-dependent re-entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re-intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.Fundação para a Ciência e a Tecnologia grants: (SRFH/BD/52172/2013, IF/0085½012/CP0185/CT0004 WP1); European Commission grants: (MCCIG321883/CCC, ERC-2014-STG-638917-ChromoCellDev); European Molecular Biology Organization grant: (IG2778).eLife Sciences PublicationsARCAPiskadlo, EwaTavares, AlexandraOliveira, Raquel A2018-01-31T13:05:19Z2017-05-062017-05-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/826engeLife 2017;6:e26120 doi: 10.7554/eLife.2612010.7554/eLife.26120info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:14Zoai:arca.igc.gulbenkian.pt:10400.7/826Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:02.818273Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
title Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
spellingShingle Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
Piskadlo, Ewa
Drosophila melanogaster
cell biology
chromosome condensation
condensin
mitosis
topoisomerase
title_short Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
title_full Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
title_fullStr Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
title_full_unstemmed Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
title_sort Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation
author Piskadlo, Ewa
author_facet Piskadlo, Ewa
Tavares, Alexandra
Oliveira, Raquel A
author_role author
author2 Tavares, Alexandra
Oliveira, Raquel A
author2_role author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Piskadlo, Ewa
Tavares, Alexandra
Oliveira, Raquel A
dc.subject.por.fl_str_mv Drosophila melanogaster
cell biology
chromosome condensation
condensin
mitosis
topoisomerase
topic Drosophila melanogaster
cell biology
chromosome condensation
condensin
mitosis
topoisomerase
description This deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://elifesciences.org/articles/26120/figures.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-06
2017-05-06T00:00:00Z
2018-01-31T13:05:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/826
url http://hdl.handle.net/10400.7/826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv eLife 2017;6:e26120 doi: 10.7554/eLife.26120
10.7554/eLife.26120
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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