Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/87273 |
Resumo: | This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyrans, with the optimum growth conditions identified as 700 μmolphotons m−2 s−1 light intensity, 1500 mg L−1 NaNO3, 6 μg L−1 CuSO4·5H2O and 40 mg L−1 NaH2PO4·H2O. These conditions allowed a 3.8-fold increase in biomass production (2.26 g AFDW per L) and improved the biochemical profile (p < 0.05) when compared with the control (Walne's medium). Namely, it was increased the contents of protein (from 10.59 to 30.76% DW), PUFAs (from 37.13 to 47.11% TFA), n − 3 FAs (26.49 to 38.27% TFA), and DHA (from 5.73 to 10.33% TFA). The nutritional value of the microalga (p < 0.05) was also improved, decreasing its atherogenic (AI – from 1.34 to 0.93) and thrombogenic (TI – from 0.45 to 0.24) indices, while the hypocholesterolemic index (HI) increased from 0.96 to 1.63. This work highlights the importance of different growth conditions in P. gyrans, playing a relevant role in the large-scale implementation of this microalga with beneficial nutritional composition for human consumption. |
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Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approachThis work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyrans, with the optimum growth conditions identified as 700 μmolphotons m−2 s−1 light intensity, 1500 mg L−1 NaNO3, 6 μg L−1 CuSO4·5H2O and 40 mg L−1 NaH2PO4·H2O. These conditions allowed a 3.8-fold increase in biomass production (2.26 g AFDW per L) and improved the biochemical profile (p < 0.05) when compared with the control (Walne's medium). Namely, it was increased the contents of protein (from 10.59 to 30.76% DW), PUFAs (from 37.13 to 47.11% TFA), n − 3 FAs (26.49 to 38.27% TFA), and DHA (from 5.73 to 10.33% TFA). The nutritional value of the microalga (p < 0.05) was also improved, decreasing its atherogenic (AI – from 1.34 to 0.93) and thrombogenic (TI – from 0.45 to 0.24) indices, while the hypocholesterolemic index (HI) increased from 0.96 to 1.63. This work highlights the importance of different growth conditions in P. gyrans, playing a relevant role in the large-scale implementation of this microalga with beneficial nutritional composition for human consumption.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UIDB/04469/2020 unit and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. This research work was supported by ALGAVALOR – Lisboa-01-0247-FEDER-035234, supported by the Operational Programme for Competitiveness and Internationalization (COMPETE2020), by the Lisbon Portugal Regional Operational Programme (Lisboa 2020) and by the Algarve Regional Operational Programme (Algarve 2020) under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Filipe Maciel acknowledges the FCT for their fellowships (SFRH/BD/133005/2017). The authors also thank the University of Aveiro, FCT, and Ministério da Ciência Tecnologia e Ensino Superior (MCTES) for the financial support to the research units CESAM (UIDB/50017/2020 + UIDP/50017/2020 + LA/P/0094/2020) and LAQV-REQUIMTE (UIDP/50006/2020 + UIDB/50006/2020) through national funds and, where applicable, co-funded by ERDF, within the Portugal 2020 Partnership Agreement and Compete 2020, and to the Portuguese Mass Spectrometry Network (RNEM, LISBOA-01-0145-FEDER-402-022125).info:eu-repo/semantics/publishedVersionRoyal Society of ChemistryUniversidade do MinhoMaciel, José Filipe GonçalvesCouto, DanielaGeada, Pedro Miguel MacedoPereira, HugoTeixeira, J. A.Domingues, M. RosárioSilva, JoanaVicente, A. A.2023-09-252023-09-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/87273engFilipe Maciel; Couto, Daniela; Geada, Pedro; Pereira, Hugo; Teixeira, José A.; Domingues, M. Rosário; Silva, Joana; Vicente, António A., Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach. Sustainable Food Technology, 1(6), 850-862, 20232753-809510.1039/D3FB00110Ehttps://pubs.rsc.org/en/journals/journalissues/fbinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-25T01:17:20Zoai:repositorium.sdum.uminho.pt:1822/87273Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:19:40.467232Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
title |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
spellingShingle |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach Maciel, José Filipe Gonçalves |
title_short |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
title_full |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
title_fullStr |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
title_full_unstemmed |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
title_sort |
Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach |
author |
Maciel, José Filipe Gonçalves |
author_facet |
Maciel, José Filipe Gonçalves Couto, Daniela Geada, Pedro Miguel Macedo Pereira, Hugo Teixeira, J. A. Domingues, M. Rosário Silva, Joana Vicente, A. A. |
author_role |
author |
author2 |
Couto, Daniela Geada, Pedro Miguel Macedo Pereira, Hugo Teixeira, J. A. Domingues, M. Rosário Silva, Joana Vicente, A. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Maciel, José Filipe Gonçalves Couto, Daniela Geada, Pedro Miguel Macedo Pereira, Hugo Teixeira, J. A. Domingues, M. Rosário Silva, Joana Vicente, A. A. |
description |
This work aimed to optimize and characterize the biomass production of Pavlova gyrans through a sequential multivariate approach. Among seventeen cultivation parameters, light intensity, NaNO3, and CuSO4·5H2O were identified as the most significative factors (p < 0.10) for biomass productivity. Together with NaH2PO4·H2O, these variables were further studied in order to maximize the biomass production of P. gyrans, with the optimum growth conditions identified as 700 μmolphotons m−2 s−1 light intensity, 1500 mg L−1 NaNO3, 6 μg L−1 CuSO4·5H2O and 40 mg L−1 NaH2PO4·H2O. These conditions allowed a 3.8-fold increase in biomass production (2.26 g AFDW per L) and improved the biochemical profile (p < 0.05) when compared with the control (Walne's medium). Namely, it was increased the contents of protein (from 10.59 to 30.76% DW), PUFAs (from 37.13 to 47.11% TFA), n − 3 FAs (26.49 to 38.27% TFA), and DHA (from 5.73 to 10.33% TFA). The nutritional value of the microalga (p < 0.05) was also improved, decreasing its atherogenic (AI – from 1.34 to 0.93) and thrombogenic (TI – from 0.45 to 0.24) indices, while the hypocholesterolemic index (HI) increased from 0.96 to 1.63. This work highlights the importance of different growth conditions in P. gyrans, playing a relevant role in the large-scale implementation of this microalga with beneficial nutritional composition for human consumption. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-25 2023-09-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/87273 |
url |
https://hdl.handle.net/1822/87273 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Filipe Maciel; Couto, Daniela; Geada, Pedro; Pereira, Hugo; Teixeira, José A.; Domingues, M. Rosário; Silva, Joana; Vicente, António A., Optimization of Pavlova gyrans biomass production and the fatty acid profile using a two-step approach. Sustainable Food Technology, 1(6), 850-862, 2023 2753-8095 10.1039/D3FB00110E https://pubs.rsc.org/en/journals/journalissues/fb |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
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