Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy

Detalhes bibliográficos
Autor(a) principal: Loureiro, Ana Isabel Sá
Data de Publicação: 2018
Outros Autores: Cavaco-Paulo, Artur
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/51296
Resumo: XII Spanish-Portuguese Conference on Controlled Drug Delivery
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spelling Folic acid-tagged protein nanoemulsions with controllable size for cancer therapyXII Spanish-Portuguese Conference on Controlled Drug DeliveryCancer is one of the most devastating diseases and the conventional chemotherapeutics agents distribute nonspecifically in the body, inducing a number of drawbacks [1, 2]. Protein-based nanoparticles have gained considerable interest as drug delivery devices due to their exceptional characteristics [3, 4]. Additionally, protein-based nanoparticles can also be easily amenable for surface modification and covalent attachment of drugs and targeting ligands [3, 5]. The aim of this work was the development of albumin nanoemulsions as drug delivery systems for cancer therapy. The production of albumin nanoemulsions was achieved by high pressure homogenization of an aqueous solution with an organic solvent (vegetable oil), subjecting the mixture to varying number of homogenization cycles at high pressure. In order to determine the best formulation for therapeutic applications, physicochemical and biological (in vitro and in vivo) characterizations were performed. Albumin nanoemulsions were produced by high pressure homogenization with and without a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size – the use of 5 mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100 nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. Folic acid (FA)-tagged protein nanoemulsions were shown to promote specific folate receptor (FR)-mediated targeting in FR positive cells. Carbon monoxide releasing molecule-2 (CORM-2) was incorporated in the oil phase of the initial formulation. FA-tagged nanoemulsions loaded with CORM-2 exhibited a considerable antitumor effect and an increased survival of BALB/c mice bearing subcutaneous A20 lymphoma tumors (Figure 1). The developed nanoemulsions also demonstrated to be well tolerated by these immunocompetent mice. The novel strategy presented here enables the construction of highly stable, size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.Ana Loureiro thanks for a scholarship (SFRH/BD/81479/2011) from Fundação para a Ciência e a Tecnologia (FCT). This work has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement NMP4-LA-2009-228827 NANOFOL. This work was supported by FEDER through POFC – COMPETE and by Portuguese funds from FCT through the project PEst-OE/BIA/UI4050/2014. The authors also thank the FCT Strategic Project of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER000004) funded by European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and the European Union Horizon 2020 research and innovation programme under grant agreement NMP-06-2015- 683356 FOLSMART.info:eu-repo/semantics/publishedVersionUniversidade do MinhoLoureiro, Ana Isabel SáCavaco-Paulo, Artur2018-012018-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/51296engLoureiro, Ana; Cavaco-Paulo, Artur, Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy. XII Spanish-Portuguese Conference on Controlled Drug Delivery. No. PC-49, Coimbra, Portugal, Jan 14-16, 105, 2018.https://www.splc-crs.org/meeting-2018/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:34:38Zoai:repositorium.sdum.uminho.pt:1822/51296Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:34:38Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
title Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
spellingShingle Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
Loureiro, Ana Isabel Sá
title_short Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
title_full Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
title_fullStr Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
title_full_unstemmed Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
title_sort Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy
author Loureiro, Ana Isabel Sá
author_facet Loureiro, Ana Isabel Sá
Cavaco-Paulo, Artur
author_role author
author2 Cavaco-Paulo, Artur
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Loureiro, Ana Isabel Sá
Cavaco-Paulo, Artur
description XII Spanish-Portuguese Conference on Controlled Drug Delivery
publishDate 2018
dc.date.none.fl_str_mv 2018-01
2018-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/51296
url http://hdl.handle.net/1822/51296
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Loureiro, Ana; Cavaco-Paulo, Artur, Folic acid-tagged protein nanoemulsions with controllable size for cancer therapy. XII Spanish-Portuguese Conference on Controlled Drug Delivery. No. PC-49, Coimbra, Portugal, Jan 14-16, 105, 2018.
https://www.splc-crs.org/meeting-2018/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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