Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems

Detalhes bibliográficos
Autor(a) principal: Madeira, Pedro
Data de Publicação: 2008
Outros Autores: Teixeira, J. A., Macedo, Eugénia, Mikheeva, Larissa M., Zaslavsky, Boris Y.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/7885
Resumo: Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation.
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spelling Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systemsAqueous two-phase systemsProtein partitioningCollander equationScience & TechnologyDistribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation.Fundação para a Ciência e a Tecnologia (FCT)FEDERElsevierUniversidade do MinhoMadeira, PedroTeixeira, J. A.Macedo, EugéniaMikheeva, Larissa M.Zaslavsky, Boris Y.2008-052008-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7885eng"Fluid Phase Equilibria". ISSN 0378-3812. 267:2 (May 2008) 150-157.0378-381210.1016/j.fluid.2008.03.003info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:20Zoai:repositorium.sdum.uminho.pt:1822/7885Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:20.341024Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
title Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
spellingShingle Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
Madeira, Pedro
Aqueous two-phase systems
Protein partitioning
Collander equation
Science & Technology
title_short Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
title_full Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
title_fullStr Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
title_full_unstemmed Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
title_sort Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systems
author Madeira, Pedro
author_facet Madeira, Pedro
Teixeira, J. A.
Macedo, Eugénia
Mikheeva, Larissa M.
Zaslavsky, Boris Y.
author_role author
author2 Teixeira, J. A.
Macedo, Eugénia
Mikheeva, Larissa M.
Zaslavsky, Boris Y.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Madeira, Pedro
Teixeira, J. A.
Macedo, Eugénia
Mikheeva, Larissa M.
Zaslavsky, Boris Y.
dc.subject.por.fl_str_mv Aqueous two-phase systems
Protein partitioning
Collander equation
Science & Technology
topic Aqueous two-phase systems
Protein partitioning
Collander equation
Science & Technology
description Distribution coefficients for a variety of proteins and certain other biomolecules (peptides, amino acids, and carbohydrates) (overall 27 different solutes) were measured in aqueous two-phase systems (ATPSs) dextran (Dex)–polyethylene glycol (PEG) and Dex–Ucon 50-HB-5100 (Ucon—a random copolymer of ethylene glycol and propylene glycol) both containing 0.15MNaCl in 0.01Mphosphate buffer, pH 7.4, at 23 ◦C. Distribution coefficients of some selected solutes were also measured in the above two-phase systems at three different polymer concentrations for each system. It was established that the distribution coefficients for all the proteins examined in the ATPSs are correlated according to the so-called Collander linear equation.
publishDate 2008
dc.date.none.fl_str_mv 2008-05
2008-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/7885
url http://hdl.handle.net/1822/7885
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Fluid Phase Equilibria". ISSN 0378-3812. 267:2 (May 2008) 150-157.
0378-3812
10.1016/j.fluid.2008.03.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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