Solute partitioning in polymer–salt ATPS: the collander equation
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/11349 |
Resumo: | The partition coefficients for several solutes (five nitrophenylated-monosaccharides and four proteins) were experimentally determined, at 23 ◦C, in three different tie-lines of two polymer–salt aqueous twophase systems (ATPS): UCON-K2HPO4 and UCON-NaH2PO4. These partition coefficients together with others obtained from the literature for five dinitrophenylated-amino acids were used to investigate the suitability of the Collander equation to correlate partition coefficients in polymer–salt ATPS. This equation was first proposed to describe the linear correlation between partition coefficients of solutes in different water–organic solvent systems. More recently, it was proved that partition coefficients for several biomolecules in polymer–polymer ATPS can also be correlated with this equation. In this work, several correlations were tested: partition coefficients obtained for different tie-lines within the same system and also partition coefficients obtained from different systems. In both cases, a linear relation was observed, despite a less satisfactory correlation was found when different ATPS were compared. Overall, it was demonstrated that the Collander equation can be used to satisfactorily correlate solute partitioning in the studied polymer–salt ATPS. |
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Solute partitioning in polymer–salt ATPS: the collander equationAqueous two-phase systemPolymer–saltCollander equationProteinsScience & TechnologyThe partition coefficients for several solutes (five nitrophenylated-monosaccharides and four proteins) were experimentally determined, at 23 ◦C, in three different tie-lines of two polymer–salt aqueous twophase systems (ATPS): UCON-K2HPO4 and UCON-NaH2PO4. These partition coefficients together with others obtained from the literature for five dinitrophenylated-amino acids were used to investigate the suitability of the Collander equation to correlate partition coefficients in polymer–salt ATPS. This equation was first proposed to describe the linear correlation between partition coefficients of solutes in different water–organic solvent systems. More recently, it was proved that partition coefficients for several biomolecules in polymer–polymer ATPS can also be correlated with this equation. In this work, several correlations were tested: partition coefficients obtained for different tie-lines within the same system and also partition coefficients obtained from different systems. In both cases, a linear relation was observed, despite a less satisfactory correlation was found when different ATPS were compared. Overall, it was demonstrated that the Collander equation can be used to satisfactorily correlate solute partitioning in the studied polymer–salt ATPS.FEDER.Fundação para a Ciência e a Tecnologia (FCT)ElsevierUniversidade do MinhoSilvério, Sara C.Rodríguez, OscarTeixeira, J. A.Macedo, Eugénia20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11349eng"Fluid Phase Equilibria". ISSN 0378-3812. 296:2 (2010) 173-177.0378-381210.1016/j.fluid.2010.04.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:25Zoai:repositorium.sdum.uminho.pt:1822/11349Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:06.478827Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Solute partitioning in polymer–salt ATPS: the collander equation |
title |
Solute partitioning in polymer–salt ATPS: the collander equation |
spellingShingle |
Solute partitioning in polymer–salt ATPS: the collander equation Silvério, Sara C. Aqueous two-phase system Polymer–salt Collander equation Proteins Science & Technology |
title_short |
Solute partitioning in polymer–salt ATPS: the collander equation |
title_full |
Solute partitioning in polymer–salt ATPS: the collander equation |
title_fullStr |
Solute partitioning in polymer–salt ATPS: the collander equation |
title_full_unstemmed |
Solute partitioning in polymer–salt ATPS: the collander equation |
title_sort |
Solute partitioning in polymer–salt ATPS: the collander equation |
author |
Silvério, Sara C. |
author_facet |
Silvério, Sara C. Rodríguez, Oscar Teixeira, J. A. Macedo, Eugénia |
author_role |
author |
author2 |
Rodríguez, Oscar Teixeira, J. A. Macedo, Eugénia |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silvério, Sara C. Rodríguez, Oscar Teixeira, J. A. Macedo, Eugénia |
dc.subject.por.fl_str_mv |
Aqueous two-phase system Polymer–salt Collander equation Proteins Science & Technology |
topic |
Aqueous two-phase system Polymer–salt Collander equation Proteins Science & Technology |
description |
The partition coefficients for several solutes (five nitrophenylated-monosaccharides and four proteins) were experimentally determined, at 23 ◦C, in three different tie-lines of two polymer–salt aqueous twophase systems (ATPS): UCON-K2HPO4 and UCON-NaH2PO4. These partition coefficients together with others obtained from the literature for five dinitrophenylated-amino acids were used to investigate the suitability of the Collander equation to correlate partition coefficients in polymer–salt ATPS. This equation was first proposed to describe the linear correlation between partition coefficients of solutes in different water–organic solvent systems. More recently, it was proved that partition coefficients for several biomolecules in polymer–polymer ATPS can also be correlated with this equation. In this work, several correlations were tested: partition coefficients obtained for different tie-lines within the same system and also partition coefficients obtained from different systems. In both cases, a linear relation was observed, despite a less satisfactory correlation was found when different ATPS were compared. Overall, it was demonstrated that the Collander equation can be used to satisfactorily correlate solute partitioning in the studied polymer–salt ATPS. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/11349 |
url |
http://hdl.handle.net/1822/11349 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Fluid Phase Equilibria". ISSN 0378-3812. 296:2 (2010) 173-177. 0378-3812 10.1016/j.fluid.2010.04.009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132972660555776 |