Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| DOI: | 10.1128/JCM.02012-15 |
| Texto Completo: | https://doi.org/10.1128/JCM.02012-15 |
Resumo: | PMID: 26400784 WOS:000367532800016 |
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7160 |
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Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South BrazilMicrobiology (medical)Infectious DiseasesBiochemistry, Genetics and Molecular Biology (miscellaneous)SDG 3 - Good Health and Well-beingPMID: 26400784 WOS:000367532800016We recently detected the spoligotype patterns of strains of Mycobacterium pinnipedii, a species of the Mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in Porto Alegre, South Brazil. Because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing, verification of the loci TbD1, RD9, pks15/1, RDRio, and fbpC, the insertion of IS6110 at a site specific to the M. tuberculosis Latin American Mediterranean (LAM) lineage, and whole-genome sequencing. The combined analysis of these markers revealed that the isolates are in fact M. tuberculosis and more specifically belong to the LAM genotype. Most of these isolates (n=8) were shown to be multidrug resistant (MDR), which prompted us to perform partial sequencing of the rpoA, rpoB, rpoC, katG, and inhA genes. Seven isolates (77.8%) carried the S315T mutation in katG, and one of these (11%) also presented the C(17)T single-nucleotide polymorphism (SNP) in inhA. Interestingly, six of the MDR isolates also presented an undescribed insertion of 12 nucleotides (CCA GAA CAA CCC) in codon 516 of rpoB. No putative compensatory mutation was found in either rpoA or rpoC. This is the first report of an M. tuberculosis LAM family strain with a convergent M. pinnipedii spoligotype. These spoligotypes are observed in genotype databases at a modest frequency, highlighting that care must be taken when identifying isolates in the M. tuberculosis complex on the basis of single genetic markers.TB, HIV and opportunistic diseases and pathogens (THOP)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNDalla Costa, Elis R.Vasconcelos, Sidra E GEsteves, Leonardo S.Gomes, Harrison M.Gomes, Lia L.Da Silva, Pedro AlmeidaPerdigão, JoãoPortugal, IsabelViveiros, MiguelMcNerney, RuthPain, ArnabClark, Taane G.Rastogi, NalinUnis, GiselaRossetti, Maria Lucia RSuffys, Philip Noel2018-05-11T22:03:18Z2015-12-012015-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttps://doi.org/10.1128/JCM.02012-15eng0095-1137PURE: 1788873http://www.scopus.com/inward/record.url?scp=84947775741&partnerID=8YFLogxKhttps://doi.org/10.1128/JCM.02012-15info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:32:36Zoai:run.unl.pt:10362/36596Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:32:36Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| title |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| spellingShingle |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil Dalla Costa, Elis R. Microbiology (medical) Infectious Diseases Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being Dalla Costa, Elis R. Microbiology (medical) Infectious Diseases Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| title_short |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| title_full |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| title_fullStr |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| title_full_unstemmed |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| title_sort |
Multidrug-resistant mycobacterium tuberculosis of the Latin American mediterranean lineage, wrongly identified as mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), causing active tuberculosis in South Brazil |
| author |
Dalla Costa, Elis R. |
| author_facet |
Dalla Costa, Elis R. Dalla Costa, Elis R. Vasconcelos, Sidra E G Esteves, Leonardo S. Gomes, Harrison M. Gomes, Lia L. Da Silva, Pedro Almeida Perdigão, João Portugal, Isabel Viveiros, Miguel McNerney, Ruth Pain, Arnab Clark, Taane G. Rastogi, Nalin Unis, Gisela Rossetti, Maria Lucia R Suffys, Philip Noel Vasconcelos, Sidra E G Esteves, Leonardo S. Gomes, Harrison M. Gomes, Lia L. Da Silva, Pedro Almeida Perdigão, João Portugal, Isabel Viveiros, Miguel McNerney, Ruth Pain, Arnab Clark, Taane G. Rastogi, Nalin Unis, Gisela Rossetti, Maria Lucia R Suffys, Philip Noel |
| author_role |
author |
| author2 |
Vasconcelos, Sidra E G Esteves, Leonardo S. Gomes, Harrison M. Gomes, Lia L. Da Silva, Pedro Almeida Perdigão, João Portugal, Isabel Viveiros, Miguel McNerney, Ruth Pain, Arnab Clark, Taane G. Rastogi, Nalin Unis, Gisela Rossetti, Maria Lucia R Suffys, Philip Noel |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
TB, HIV and opportunistic diseases and pathogens (THOP) Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) RUN |
| dc.contributor.author.fl_str_mv |
Dalla Costa, Elis R. Vasconcelos, Sidra E G Esteves, Leonardo S. Gomes, Harrison M. Gomes, Lia L. Da Silva, Pedro Almeida Perdigão, João Portugal, Isabel Viveiros, Miguel McNerney, Ruth Pain, Arnab Clark, Taane G. Rastogi, Nalin Unis, Gisela Rossetti, Maria Lucia R Suffys, Philip Noel |
| dc.subject.por.fl_str_mv |
Microbiology (medical) Infectious Diseases Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| topic |
Microbiology (medical) Infectious Diseases Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| description |
PMID: 26400784 WOS:000367532800016 |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-12-01 2015-12-01T00:00:00Z 2018-05-11T22:03:18Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1128/JCM.02012-15 |
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https://doi.org/10.1128/JCM.02012-15 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0095-1137 PURE: 1788873 http://www.scopus.com/inward/record.url?scp=84947775741&partnerID=8YFLogxK https://doi.org/10.1128/JCM.02012-15 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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7 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1822181943867867136 |
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10.1128/JCM.02012-15 |