A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/6466 |
Resumo: | Supplementary data to this article can be found online at https://doi.org/10.1016/j.bbrc.2019.08.105. |
| id |
RCAP_1562b2733f90e70a31dcb9efd1766199 |
|---|---|
| oai_identifier_str |
oai:repositorio.insa.pt:10400.18/6466 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cellsNonsense-mediated mRNA DecayNMDDIS3L2Terminal Uridylyl Transferases Zcchc6/11TUT7/4mRNA TurnoverDoenças GenéticasGenómica Funcional e EstruturalSupplementary data to this article can be found online at https://doi.org/10.1016/j.bbrc.2019.08.105.The nonsense-mediated decay (NMD) pathway selectively degrades mRNAs carrying a premature translation-termination codon but also regulates the abundance of a large number of physiological mRNAs that encode full-length proteins. In human cells, NMD-targeted mRNAs are degraded by endonucleolytic cleavage and exonucleolytic degradation from both 5-' and 3'-ends. This is done by a process not yet completely understood that recruits decapping and 5'-to-3' exonuclease activities, as well as deadenylating and 3'-to-5' exonuclease exosome activities. In yeast, DIS3/Rrp44 protein is the catalytic subunit of the exosome, but in humans, there are three known paralogues of this enzyme: DIS3, DIS3L1, and DIS3L2. However, little is known about their role in NMD. Here, we show that some NMD-targets are DIS3L2 substrates in human cells. In addition, we observed that DIS3L2 acts over full-length transcripts, through a process that also involves UPF1. Moreover, DIS3L2-mediated decay is dependent on the activity of the terminal uridylyl transferases Zcchc6/11 (TUT7/4). Together, our findings establish a role for DIS3L2 and uridylation in NMD.Highlights: DIS3L2 functions in the decay of natural NMD-targets in a transcript-specific manner; DIS3L2 acts over full-length NMD-targets, through a process that also involves UPF1; DIS3L2 function in NMD is dependent on the terminal uridylyl transferases Zcchc6/11.This work was partially supported by Fundaçao para a Ci ~ encia e ^ a Tecnologia (FCT) (PTFC/BIM-MEC/3749/2014 to LR and UID/ MULTI/04046/2013 to BioISI). PJdC, HAS and JFG-M are recipients of a fellowship from BioSys PhD programme (SFRH/BD/52495/2014, SFRH/BD/52492/2014, and PD/BD/142898/2018, respectively) and JM is a posdoc fellow (SFRH/BPD/98360/2013) from FCT. Work at ITQB-NOVA was financially supported by: Project LISBOA-01-0145-FEDER-007660 funded by the European Regional Development Fund (FEDER) through COMPETE2020 - Programa Operacional Competitividade e Internacionalizaçao (POCI) and by FCT funds: ~ PTDC/BIA-MIC/1399/2014 to CMA and PTDC/BIM-MEC/3749/2014 to SCV. SCV was financed by program IF of FCT (IF/00217/2015). MS was financed by an FCT contract according to DL57/2016 [SFRH/ BPD/109464/2015]. We thank Dr. V. Narry Kim from Seoul National University, who kindly provided us with the pCK-FLAG-TUT4 and pCK-FLAG-TUT7 vectors.Elsevier/ Academic PressRepositório Científico do Instituto Nacional de SaúdeCosta, Paulo J. daMenezes, JulianeSaramago, MargaridaGarcía-Moreno, Juan F.Santos, Hugo A.Gama-Carvalho, MargaridaArraiano, Cecília M.Romão, Luísa2020-10-22T00:30:13Z2019-10-222019-10-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6466engBiochem Biophys Res Commun. 2019 Oct 22;518(4):664-671. doi: 10.1016/j.bbrc.2019.08.105. Epub 2019 Aug 26.0006-291X10.1016/j.bbrc.2019.08.105info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:35Zoai:repositorio.insa.pt:10400.18/6466Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:21.825119Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| title |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| spellingShingle |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells Costa, Paulo J. da Nonsense-mediated mRNA Decay NMD DIS3L2 Terminal Uridylyl Transferases Zcchc6/11 TUT7/4 mRNA Turnover Doenças Genéticas Genómica Funcional e Estrutural |
| title_short |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| title_full |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| title_fullStr |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| title_full_unstemmed |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| title_sort |
A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells |
| author |
Costa, Paulo J. da |
| author_facet |
Costa, Paulo J. da Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Romão, Luísa |
| author_role |
author |
| author2 |
Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Romão, Luísa |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Costa, Paulo J. da Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Romão, Luísa |
| dc.subject.por.fl_str_mv |
Nonsense-mediated mRNA Decay NMD DIS3L2 Terminal Uridylyl Transferases Zcchc6/11 TUT7/4 mRNA Turnover Doenças Genéticas Genómica Funcional e Estrutural |
| topic |
Nonsense-mediated mRNA Decay NMD DIS3L2 Terminal Uridylyl Transferases Zcchc6/11 TUT7/4 mRNA Turnover Doenças Genéticas Genómica Funcional e Estrutural |
| description |
Supplementary data to this article can be found online at https://doi.org/10.1016/j.bbrc.2019.08.105. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-10-22 2019-10-22T00:00:00Z 2020-10-22T00:30:13Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6466 |
| url |
http://hdl.handle.net/10400.18/6466 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Biochem Biophys Res Commun. 2019 Oct 22;518(4):664-671. doi: 10.1016/j.bbrc.2019.08.105. Epub 2019 Aug 26. 0006-291X 10.1016/j.bbrc.2019.08.105 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier/ Academic Press |
| publisher.none.fl_str_mv |
Elsevier/ Academic Press |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799132158106796032 |