Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

Detalhes bibliográficos
Autor(a) principal: da Costa, Paulo J.
Data de Publicação: 2019
Outros Autores: Menezes, Juliane, Saramago, Margarida, García-Moreno, Juan F., Santos, Hugo A., Gama-Carvalho, Margarida, Arraiano, Cecília M., Viegas, Sandra C., Romão, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6501
Resumo: In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.
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spelling Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cellsDIS3L2NMDNMD-targetsUPF1mRNA DegradationmRNA SurveillanceDoenças GenéticasGenómica Funcional e EstruturalIn this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.This work was partially supported by Fundação para a Ciencia e a Tecnologia (FCT) (PTFC/BIM-MEC/3749/2014 to LR and UID/MULTI/04046/2013 to BioISI). PJdC, HAS and JFG-M are recipients of a fellowship from BioSys PhD programme (SFRH/BD/52495/2014, SFRH/BD/52492/2014, and PD/BD/ 142898/2018, respectively) and JM is a postdoctoral fellow (SFRH/BPD/98360/2013) from FCT. Work at ITQB-NOVA was financially supported by: Project LISBOA-01-0145-FEDER-007660 funded by the European Regional Development Fund (FEDER) through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by FCT funds: PTDC/BIA-MIC/1399/2014 to CMA and PTFC/BIM-MEC/3749/2014 to SCV. SCV was financed by program IF of FCT (IF/00217/2015). MS was financed by an FCT contract according to DL57/2016 [SFRH/BPD/109464/2015]ElsevierRepositório Científico do Instituto Nacional de Saúdeda Costa, Paulo J.Menezes, JulianeSaramago, MargaridaGarcía-Moreno, Juan F.Santos, Hugo A.Gama-Carvalho, MargaridaArraiano, Cecília M.Viegas, Sandra C.Romão, Luísa2020-04-23T18:11:13Z2019-12-062019-12-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6501engData Brief . 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb2352-340910.1016/j.dib.2019.104943info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:35Zoai:repositorio.insa.pt:10400.18/6501Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:22.202187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
title Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
spellingShingle Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
da Costa, Paulo J.
DIS3L2
NMD
NMD-targets
UPF1
mRNA Degradation
mRNA Surveillance
Doenças Genéticas
Genómica Funcional e Estrutural
title_short Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
title_full Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
title_fullStr Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
title_full_unstemmed Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
title_sort Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
author da Costa, Paulo J.
author_facet da Costa, Paulo J.
Menezes, Juliane
Saramago, Margarida
García-Moreno, Juan F.
Santos, Hugo A.
Gama-Carvalho, Margarida
Arraiano, Cecília M.
Viegas, Sandra C.
Romão, Luísa
author_role author
author2 Menezes, Juliane
Saramago, Margarida
García-Moreno, Juan F.
Santos, Hugo A.
Gama-Carvalho, Margarida
Arraiano, Cecília M.
Viegas, Sandra C.
Romão, Luísa
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv da Costa, Paulo J.
Menezes, Juliane
Saramago, Margarida
García-Moreno, Juan F.
Santos, Hugo A.
Gama-Carvalho, Margarida
Arraiano, Cecília M.
Viegas, Sandra C.
Romão, Luísa
dc.subject.por.fl_str_mv DIS3L2
NMD
NMD-targets
UPF1
mRNA Degradation
mRNA Surveillance
Doenças Genéticas
Genómica Funcional e Estrutural
topic DIS3L2
NMD
NMD-targets
UPF1
mRNA Degradation
mRNA Surveillance
Doenças Genéticas
Genómica Funcional e Estrutural
description In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-06
2019-12-06T00:00:00Z
2020-04-23T18:11:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6501
url http://hdl.handle.net/10400.18/6501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Data Brief . 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb
2352-3409
10.1016/j.dib.2019.104943
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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