Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6501 |
Resumo: | In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism. |
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Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cellsDIS3L2NMDNMD-targetsUPF1mRNA DegradationmRNA SurveillanceDoenças GenéticasGenómica Funcional e EstruturalIn this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.This work was partially supported by Fundação para a Ciencia e a Tecnologia (FCT) (PTFC/BIM-MEC/3749/2014 to LR and UID/MULTI/04046/2013 to BioISI). PJdC, HAS and JFG-M are recipients of a fellowship from BioSys PhD programme (SFRH/BD/52495/2014, SFRH/BD/52492/2014, and PD/BD/ 142898/2018, respectively) and JM is a postdoctoral fellow (SFRH/BPD/98360/2013) from FCT. Work at ITQB-NOVA was financially supported by: Project LISBOA-01-0145-FEDER-007660 funded by the European Regional Development Fund (FEDER) through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by FCT funds: PTDC/BIA-MIC/1399/2014 to CMA and PTFC/BIM-MEC/3749/2014 to SCV. SCV was financed by program IF of FCT (IF/00217/2015). MS was financed by an FCT contract according to DL57/2016 [SFRH/BPD/109464/2015]ElsevierRepositório Científico do Instituto Nacional de Saúdeda Costa, Paulo J.Menezes, JulianeSaramago, MargaridaGarcía-Moreno, Juan F.Santos, Hugo A.Gama-Carvalho, MargaridaArraiano, Cecília M.Viegas, Sandra C.Romão, Luísa2020-04-23T18:11:13Z2019-12-062019-12-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6501engData Brief . 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb2352-340910.1016/j.dib.2019.104943info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:35Zoai:repositorio.insa.pt:10400.18/6501Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:22.202187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
title |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
spellingShingle |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells da Costa, Paulo J. DIS3L2 NMD NMD-targets UPF1 mRNA Degradation mRNA Surveillance Doenças Genéticas Genómica Funcional e Estrutural |
title_short |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
title_full |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
title_fullStr |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
title_full_unstemmed |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
title_sort |
Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells |
author |
da Costa, Paulo J. |
author_facet |
da Costa, Paulo J. Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Viegas, Sandra C. Romão, Luísa |
author_role |
author |
author2 |
Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Viegas, Sandra C. Romão, Luísa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
da Costa, Paulo J. Menezes, Juliane Saramago, Margarida García-Moreno, Juan F. Santos, Hugo A. Gama-Carvalho, Margarida Arraiano, Cecília M. Viegas, Sandra C. Romão, Luísa |
dc.subject.por.fl_str_mv |
DIS3L2 NMD NMD-targets UPF1 mRNA Degradation mRNA Surveillance Doenças Genéticas Genómica Funcional e Estrutural |
topic |
DIS3L2 NMD NMD-targets UPF1 mRNA Degradation mRNA Surveillance Doenças Genéticas Genómica Funcional e Estrutural |
description |
In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-06 2019-12-06T00:00:00Z 2020-04-23T18:11:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6501 |
url |
http://hdl.handle.net/10400.18/6501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Data Brief . 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Feb 2352-3409 10.1016/j.dib.2019.104943 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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