A new PNA-FISH probe targeting Fannyhessea vaginae

Detalhes bibliográficos
Autor(a) principal: Sousa, Lúcia Filipa Guimarães Vieira
Data de Publicação: 2021
Outros Autores: Castro, Joana Isabel Reis, França, Ângela Maria Oliveira Sousa, Almeida, Carina, Muzny, C. A., Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/75262
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.779376/full#supplementary-material
id RCAP_15a9bf196ab30d235de3de4610a8b6ac
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/75262
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling A new PNA-FISH probe targeting Fannyhessea vaginaeFannyhessea vaginaeGardnerella vaginalisbacterial vaginosisfluorescence in situ hybridization (FISH)peptide nucleic acid (PNA)Ciências Naturais::Ciências BiológicasScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.779376/full#supplementary-materialBacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood. Gardnerella spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless, Fannyhessea vaginae (previously known as Atopobium vaginae) is a highly specific species for BV, and co-colonization with Gardnerella is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting F. vaginae. This probe was further validated in a multiplex assay, which included a Gardnerella-specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate F. vaginae-Gardnerella biofilms, confirming the potential to be used for the detection of BV-associated pathogens.This work was funded by the National Institute of Allergy and Infectious Diseases (R01AI146065-01A1). It was also partially funded by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of unit (UIDB/04469/2020).info:eu-repo/semantics/publishedVersionFrontiers Media S. A.Universidade do MinhoSousa, Lúcia Filipa Guimarães VieiraCastro, Joana Isabel ReisFrança, Ângela Maria Oliveira SousaAlmeida, CarinaMuzny, C. A.Cerca, Nuno2021-11-182021-11-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/75262engSousa, Lúcia; Castro, Joana; França, Angela; Almeida, Carina; Muzny, C. A.; Cerca, Nuno, A new PNA-FISH probe targeting Fannyhessea vaginae. Frontiers in Cellular and Infection Microbiology, 11(779376), 20212235-298810.3389/fcimb.2021.77937634869078779376https://www.frontiersin.org/journals/cellular-and-infection-microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:56Zoai:repositorium.sdum.uminho.pt:1822/75262Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:08.055356Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new PNA-FISH probe targeting Fannyhessea vaginae
title A new PNA-FISH probe targeting Fannyhessea vaginae
spellingShingle A new PNA-FISH probe targeting Fannyhessea vaginae
Sousa, Lúcia Filipa Guimarães Vieira
Fannyhessea vaginae
Gardnerella vaginalis
bacterial vaginosis
fluorescence in situ hybridization (FISH)
peptide nucleic acid (PNA)
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short A new PNA-FISH probe targeting Fannyhessea vaginae
title_full A new PNA-FISH probe targeting Fannyhessea vaginae
title_fullStr A new PNA-FISH probe targeting Fannyhessea vaginae
title_full_unstemmed A new PNA-FISH probe targeting Fannyhessea vaginae
title_sort A new PNA-FISH probe targeting Fannyhessea vaginae
author Sousa, Lúcia Filipa Guimarães Vieira
author_facet Sousa, Lúcia Filipa Guimarães Vieira
Castro, Joana Isabel Reis
França, Ângela Maria Oliveira Sousa
Almeida, Carina
Muzny, C. A.
Cerca, Nuno
author_role author
author2 Castro, Joana Isabel Reis
França, Ângela Maria Oliveira Sousa
Almeida, Carina
Muzny, C. A.
Cerca, Nuno
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sousa, Lúcia Filipa Guimarães Vieira
Castro, Joana Isabel Reis
França, Ângela Maria Oliveira Sousa
Almeida, Carina
Muzny, C. A.
Cerca, Nuno
dc.subject.por.fl_str_mv Fannyhessea vaginae
Gardnerella vaginalis
bacterial vaginosis
fluorescence in situ hybridization (FISH)
peptide nucleic acid (PNA)
Ciências Naturais::Ciências Biológicas
Science & Technology
topic Fannyhessea vaginae
Gardnerella vaginalis
bacterial vaginosis
fluorescence in situ hybridization (FISH)
peptide nucleic acid (PNA)
Ciências Naturais::Ciências Biológicas
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.779376/full#supplementary-material
publishDate 2021
dc.date.none.fl_str_mv 2021-11-18
2021-11-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/75262
url https://hdl.handle.net/1822/75262
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sousa, Lúcia; Castro, Joana; França, Angela; Almeida, Carina; Muzny, C. A.; Cerca, Nuno, A new PNA-FISH probe targeting Fannyhessea vaginae. Frontiers in Cellular and Infection Microbiology, 11(779376), 2021
2235-2988
10.3389/fcimb.2021.779376
34869078
779376
https://www.frontiersin.org/journals/cellular-and-infection-microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S. A.
publisher.none.fl_str_mv Frontiers Media S. A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132847788785664

Registros relacionados