Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis

Detalhes bibliográficos
Autor(a) principal: Castro, Joana Isabel Reis
Data de Publicação: 2022
Outros Autores: Sousa, Lúcia, França, Ângela Maria Oliveira Sousa, Tisakova, Lenka Podpera, Corsini, Lorenzo, Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/77253
Resumo: Supplementary Materials: The following are available online at: https://www.mdpi.com/article/10.3390/antibiotics11050558/s1, Figure S1: An example data set on the organization of the dual-species BV-associated biofilms by epifluorescence microscopy, Table S1: Bacterial strains used in this study, Table S2: Specific primers for the quantification of the bacterial species present in the dual-species biofilm.
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spelling Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosisbacterial vaginosisGardnerella vaginalisFannyhessea vaginaePrevotella biviaantimicrobial resistancealternative therapiesendolysinanti-biofilmScience & TechnologySupplementary Materials: The following are available online at: https://www.mdpi.com/article/10.3390/antibiotics11050558/s1, Figure S1: An example data set on the organization of the dual-species BV-associated biofilms by epifluorescence microscopy, Table S1: Bacterial strains used in this study, Table S2: Specific primers for the quantification of the bacterial species present in the dual-species biofilm.Bacterial vaginosis (BV) is the most frequent vaginal infection in women of reproductive age. It is caused by the overgrowth of anaerobic vaginal pathogens, such as Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia, which are vaginal pathogens detected during the early stages of incident BV and have been found to form multi-species biofilms. Treatment of biofilm-associated infections, such as BV, is challenging. In this study, we tested the role of an investigational engineered phage endolysin, PM-477, in the eradication of dual-species biofilms composed of G. vaginalis–F. vaginae or G. vaginalis–P. bivia. Single-species biofilms formed by these species were also analysed as controls. The effect of PM-477 on biomass and culturability of single- and dual-species biofilms was assessed in vitro using a microtiter plate assay, epifluorescence microscopy, confocal laser scanning microscopy, and quantitative PCR. The results showed that PM-477 was particularly effective in the disruption and reduction of culturability of G. vaginalis biofilms. In dual-species biofilms, PM-477 exhibited lower efficiency but was still able to selectively and significantly eliminate G. vaginalis. Since polymicrobial interactions have been shown to strongly affect the activity of various antibiotics, the activity of PM-477 in dual-species biofilms is a potentially promising result that should be further explored, aiming to completely eradicate multi-species biofilms associated with BV.This research was partially funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of unit (UIDB/04469/2020). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoCastro, Joana Isabel ReisSousa, LúciaFrança, Ângela Maria Oliveira SousaTisakova, Lenka PodperaCorsini, LorenzoCerca, Nuno2022-04-222022-04-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/77253engCastro, J.; Sousa, L.G.V.; França, Â.; Tisakova, L.P.; Corsini, L.; Cerca, N. Exploiting the Anti-Biofilm Effect of the Engineered Phage Endolysin PM-477 to Disrupt In Vitro Single- and Dual-Species Biofilms of Vaginal Pathogens Associated with Bacterial Vaginosis. Antibiotics 2022, 11, 558. https://doi.org/10.3390/antibiotics110505582079-63822079-638210.3390/antibiotics11050558https://www.mdpi.com/2079-6382/11/5/558info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:39Zoai:repositorium.sdum.uminho.pt:1822/77253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:18.510431Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
title Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
spellingShingle Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
Castro, Joana Isabel Reis
bacterial vaginosis
Gardnerella vaginalis
Fannyhessea vaginae
Prevotella bivia
antimicrobial resistance
alternative therapies
endolysin
anti-biofilm
Science & Technology
title_short Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
title_full Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
title_fullStr Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
title_full_unstemmed Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
title_sort Exploiting the anti-biofilm effect of the engineered phage endolysin PM-477 to disrupt in vitro single- and dual-species biofilms of vaginal pathogens associated with bacterial vaginosis
author Castro, Joana Isabel Reis
author_facet Castro, Joana Isabel Reis
Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Tisakova, Lenka Podpera
Corsini, Lorenzo
Cerca, Nuno
author_role author
author2 Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Tisakova, Lenka Podpera
Corsini, Lorenzo
Cerca, Nuno
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Castro, Joana Isabel Reis
Sousa, Lúcia
França, Ângela Maria Oliveira Sousa
Tisakova, Lenka Podpera
Corsini, Lorenzo
Cerca, Nuno
dc.subject.por.fl_str_mv bacterial vaginosis
Gardnerella vaginalis
Fannyhessea vaginae
Prevotella bivia
antimicrobial resistance
alternative therapies
endolysin
anti-biofilm
Science & Technology
topic bacterial vaginosis
Gardnerella vaginalis
Fannyhessea vaginae
Prevotella bivia
antimicrobial resistance
alternative therapies
endolysin
anti-biofilm
Science & Technology
description Supplementary Materials: The following are available online at: https://www.mdpi.com/article/10.3390/antibiotics11050558/s1, Figure S1: An example data set on the organization of the dual-species BV-associated biofilms by epifluorescence microscopy, Table S1: Bacterial strains used in this study, Table S2: Specific primers for the quantification of the bacterial species present in the dual-species biofilm.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-22
2022-04-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/77253
url https://hdl.handle.net/1822/77253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro, J.; Sousa, L.G.V.; França, Â.; Tisakova, L.P.; Corsini, L.; Cerca, N. Exploiting the Anti-Biofilm Effect of the Engineered Phage Endolysin PM-477 to Disrupt In Vitro Single- and Dual-Species Biofilms of Vaginal Pathogens Associated with Bacterial Vaginosis. Antibiotics 2022, 11, 558. https://doi.org/10.3390/antibiotics11050558
2079-6382
2079-6382
10.3390/antibiotics11050558
https://www.mdpi.com/2079-6382/11/5/558
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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