Molecular techniques for dicistrovirus detection without RNA extraction or purification.

Detalhes bibliográficos
Autor(a) principal: Silva, Marcelo
Data de Publicação: 2013
Tipo de documento: Artigo
Idioma: und
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116942
Resumo: Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
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spelling Molecular techniques for dicistrovirus detection without RNA extraction or purification.SDG 3 - Good Health and Well-beingDicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.Centro de Malária e outras Doenças Tropicais (CMDT)RUNSilva, Marcelo2021-05-04T22:31:25Z2013-01-012013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/116942und2314-6133PURE: 169908https://doi.org/10.1155/2013/218593info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:50Zoai:run.unl.pt:10362/116942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:23.441472Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular techniques for dicistrovirus detection without RNA extraction or purification.
title Molecular techniques for dicistrovirus detection without RNA extraction or purification.
spellingShingle Molecular techniques for dicistrovirus detection without RNA extraction or purification.
Silva, Marcelo
SDG 3 - Good Health and Well-being
title_short Molecular techniques for dicistrovirus detection without RNA extraction or purification.
title_full Molecular techniques for dicistrovirus detection without RNA extraction or purification.
title_fullStr Molecular techniques for dicistrovirus detection without RNA extraction or purification.
title_full_unstemmed Molecular techniques for dicistrovirus detection without RNA extraction or purification.
title_sort Molecular techniques for dicistrovirus detection without RNA extraction or purification.
author Silva, Marcelo
author_facet Silva, Marcelo
author_role author
dc.contributor.none.fl_str_mv Centro de Malária e outras Doenças Tropicais (CMDT)
RUN
dc.contributor.author.fl_str_mv Silva, Marcelo
dc.subject.por.fl_str_mv SDG 3 - Good Health and Well-being
topic SDG 3 - Good Health and Well-being
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2013-01-01T00:00:00Z
2021-05-04T22:31:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116942
url http://hdl.handle.net/10362/116942
dc.language.iso.fl_str_mv und
language und
dc.relation.none.fl_str_mv 2314-6133
PURE: 169908
https://doi.org/10.1155/2013/218593
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eu_rights_str_mv openAccess
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