Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/2559 |
Resumo: | Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains. |
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Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strainCarbon sourceFlocculationNewFlo phenotypeProtein synthesisSaccharomyces cerevisiaeScience & TechnologyAims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains.This work was supported by Fundo de Apoio à Investigação from Instituto Polite´cnico do Porto (Project P24/96 and P24/97) and by Programa Plurianual de Unidades de I&D CIEA/ISEP. Ann Vroman (KdGH-Antwerpen, Belgium), Jan Mortier and Katleen Rijsbrack (KaHo St Lieven, Belgium) wishes to thank Dr Rob Gille´ and Dr C. Van Keer for the opportunity to participate in the ERASMUS bilateral agreement programme between their schools and ISEP (Portugal).Blackwell PublishingUniversidade do MinhoSoares, Eduardo V.Vroman, A.Mortier, J.Rijsbrack, K.Mota, M.2004-052004-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/2559engSoares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley. http://doi.org/10.1111/j.1365-2672.2004.02240.x1364-50721365-267210.1111/j.1365-2672.2004.02240.x15078529https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02240.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:24Zoai:repositorium.sdum.uminho.pt:1822/2559Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:23.579393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
title |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
spellingShingle |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain Soares, Eduardo V. Carbon source Flocculation NewFlo phenotype Protein synthesis Saccharomyces cerevisiae Science & Technology |
title_short |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
title_full |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
title_fullStr |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
title_full_unstemmed |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
title_sort |
Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain |
author |
Soares, Eduardo V. |
author_facet |
Soares, Eduardo V. Vroman, A. Mortier, J. Rijsbrack, K. Mota, M. |
author_role |
author |
author2 |
Vroman, A. Mortier, J. Rijsbrack, K. Mota, M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Soares, Eduardo V. Vroman, A. Mortier, J. Rijsbrack, K. Mota, M. |
dc.subject.por.fl_str_mv |
Carbon source Flocculation NewFlo phenotype Protein synthesis Saccharomyces cerevisiae Science & Technology |
topic |
Carbon source Flocculation NewFlo phenotype Protein synthesis Saccharomyces cerevisiae Science & Technology |
description |
Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-05 2004-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/2559 |
url |
https://hdl.handle.net/1822/2559 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Soares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley. http://doi.org/10.1111/j.1365-2672.2004.02240.x 1364-5072 1365-2672 10.1111/j.1365-2672.2004.02240.x 15078529 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02240.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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