Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion

Detalhes bibliográficos
Autor(a) principal: Lima, Nelson
Data de Publicação: 1995
Outros Autores: Moreira, C., Teixeira, J. A., Mota, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32677
Resumo: Protoplast fusion was applied to obtain intraspecific fusion in yeast strains in order to overcome restrictions imposed by the natural mating system. Acriflavine was used to construct petite mutants from non-flocculent industrial Saccharomyces cerevisiae saké strain. UV radiation was used to construct ura- mutants from a respiratory competent and highly flocculent S. cerevisiae NCYC869 strain. Fusion products were selected by complementation on minimal medium. The frequency of appearance of prototrophic hybrids was five per 10(5) protoplasts. The stable hybrids showed not only the S. cerevisiae saké characteristics but also flocculation ability, thereby confirming the success of the fusion.
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spelling Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusionProtoplast fusionFlocculationSaccharomyces cerevisiae sakeScience & TechnologyProtoplast fusion was applied to obtain intraspecific fusion in yeast strains in order to overcome restrictions imposed by the natural mating system. Acriflavine was used to construct petite mutants from non-flocculent industrial Saccharomyces cerevisiae saké strain. UV radiation was used to construct ura- mutants from a respiratory competent and highly flocculent S. cerevisiae NCYC869 strain. Fusion products were selected by complementation on minimal medium. The frequency of appearance of prototrophic hybrids was five per 10(5) protoplasts. The stable hybrids showed not only the S. cerevisiae saké characteristics but also flocculation ability, thereby confirming the success of the fusion.The Faculty PressUniversidade do MinhoLima, NelsonMoreira, C.Teixeira, J. A.Mota, M.19951995-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32677engLima, Nelson; Moreira, C.; Teixeira, J. A.; Mota, M., Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion. Microbios, 81, 187-197, 19950026-26337752958info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:01Zoai:repositorium.sdum.uminho.pt:1822/32677Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:54.851228Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
title Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
spellingShingle Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
Lima, Nelson
Protoplast fusion
Flocculation
Saccharomyces cerevisiae sake
Science & Technology
title_short Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
title_full Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
title_fullStr Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
title_full_unstemmed Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
title_sort Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion
author Lima, Nelson
author_facet Lima, Nelson
Moreira, C.
Teixeira, J. A.
Mota, M.
author_role author
author2 Moreira, C.
Teixeira, J. A.
Mota, M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lima, Nelson
Moreira, C.
Teixeira, J. A.
Mota, M.
dc.subject.por.fl_str_mv Protoplast fusion
Flocculation
Saccharomyces cerevisiae sake
Science & Technology
topic Protoplast fusion
Flocculation
Saccharomyces cerevisiae sake
Science & Technology
description Protoplast fusion was applied to obtain intraspecific fusion in yeast strains in order to overcome restrictions imposed by the natural mating system. Acriflavine was used to construct petite mutants from non-flocculent industrial Saccharomyces cerevisiae saké strain. UV radiation was used to construct ura- mutants from a respiratory competent and highly flocculent S. cerevisiae NCYC869 strain. Fusion products were selected by complementation on minimal medium. The frequency of appearance of prototrophic hybrids was five per 10(5) protoplasts. The stable hybrids showed not only the S. cerevisiae saké characteristics but also flocculation ability, thereby confirming the success of the fusion.
publishDate 1995
dc.date.none.fl_str_mv 1995
1995-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32677
url http://hdl.handle.net/1822/32677
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lima, Nelson; Moreira, C.; Teixeira, J. A.; Mota, M., Introduction of flocculation into industrial yeast, Saccharomyces cerevisiae saké, by protoplast fusion. Microbios, 81, 187-197, 1995
0026-2633
7752958
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Faculty Press
publisher.none.fl_str_mv The Faculty Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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