Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

Detalhes bibliográficos
Autor(a) principal: Baptista, Sílvia
Data de Publicação: 2022
Outros Autores: Torres, Cristiana A. V., Sevrin, Chantal, Grandfils, Christian, Reis, Maria A. M., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/134186
Resumo: LA/P/0140/2020
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spelling Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer PropertiesDiafiltrationEmulsionExopolysaccharideExtractionFucopolRheologyUltrafiltrationChemistry(all)Polymers and PlasticsLA/P/0140/2020In this study, membrane-based methods were evaluated for the recovery of FucoPol, the fucose-rich exopolysaccharide (EPS) secreted by the bacterium Enterobacter A47, aiming at reducing the total water consumption and extraction time, while keeping a high product recovery, thus making the downstream procedure more sustainable and cost-effective. The optimized method involved ultrafiltration of the cell-free supernatant using a 30 kDa molecular weight cut-off (MWCO) membrane that allowed for a 37% reduction of the total water consumption and a 55% reduction of the extraction time, compared to the previously used method (diafiltration-ultrafiltration with a 100 kDa MWCO membrane). This change in the downstream procedure improved the product’s recovery (around 10% increase) and its purity, evidenced by the lower protein (8.2 wt%) and inorganic salts (4.0 wt%) contents of the samples (compared to 9.3 and 8.6 wt%, respectively, for the previously used method), without impacting FucoPol’s sugar and acyl groups composition, molecular mass distribution or thermal degradation profile. The biopolymer’s emulsion-forming and stabilizing capacity was also not affected (emulsification activity (EA) with olive oil, at a 2:3 ratio, of 98 ± 0% for all samples), while the rheological properties were improved (the zero-shear viscosity increased from 8.89 ± 0.62 Pa·s to 17.40 ± 0.04 Pa·s), which can be assigned to the higher purity degree of the extracted samples. These findings demonstrate a significant improvement in the downstream procedure raising FucoPol’s recovery, while reducing water consumption and operation time, key criteria in terms of process economic and environmental sustainability. Moreover, those changes improved the biopolymer’s rheological properties, known to significantly impact FucoPol’s utilization in cosmetic, pharmaceutical or food products.DQ - Departamento de QuímicaLAQV@REQUIMTEUCIBIO - Applied Molecular Biosciences UnitRUNBaptista, SílviaTorres, Cristiana A. V.Sevrin, ChantalGrandfils, ChristianReis, Maria A. M.Freitas, Filomena2022-03-09T23:24:39Z2022-02-012022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/134186engPURE: 36506473https://doi.org/10.3390/polym14030390info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:12:37Zoai:run.unl.pt:10362/134186Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:00.387176Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
title Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
spellingShingle Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
Baptista, Sílvia
Diafiltration
Emulsion
Exopolysaccharide
Extraction
Fucopol
Rheology
Ultrafiltration
Chemistry(all)
Polymers and Plastics
title_short Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
title_full Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
title_fullStr Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
title_full_unstemmed Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
title_sort Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties
author Baptista, Sílvia
author_facet Baptista, Sílvia
Torres, Cristiana A. V.
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Freitas, Filomena
author_role author
author2 Torres, Cristiana A. V.
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Freitas, Filomena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Baptista, Sílvia
Torres, Cristiana A. V.
Sevrin, Chantal
Grandfils, Christian
Reis, Maria A. M.
Freitas, Filomena
dc.subject.por.fl_str_mv Diafiltration
Emulsion
Exopolysaccharide
Extraction
Fucopol
Rheology
Ultrafiltration
Chemistry(all)
Polymers and Plastics
topic Diafiltration
Emulsion
Exopolysaccharide
Extraction
Fucopol
Rheology
Ultrafiltration
Chemistry(all)
Polymers and Plastics
description LA/P/0140/2020
publishDate 2022
dc.date.none.fl_str_mv 2022-03-09T23:24:39Z
2022-02-01
2022-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/134186
url http://hdl.handle.net/10362/134186
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 36506473
https://doi.org/10.3390/polym14030390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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