Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Correia, Elisete, Pereira, José Eduardo, González-Machado, Camino, Capita, Rosa, Alonso-Calleja, Carlos, Igrejas, Gilberto, Poeta, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143555
Resumo: NORTE-01-0145-FEDER-030101 UIDB/CVT/00772/2020 LA/P/0059/2020 RTI2018-098267-R-C33 LE018P20
id RCAP_17ada100a150e3e105146706c344c32f
oai_identifier_str oai:run.unl.pt:10362/143555
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Speciesbiofilmcoagulase-negative staphylococci (CoNS)staphylococciImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingNORTE-01-0145-FEDER-030101 UIDB/CVT/00772/2020 LA/P/0059/2020 RTI2018-098267-R-C33 LE018P20The ability of biofilm formation seems to play an important role in the virulence of staphylococci. However, studies reporting biofilm formation of coagulase-negative staphylococci isolated from animals are still very scarce. Thus, we aimed to evaluate the biofilm-forming capacity of CoNS and S. pseudintermedius isolated from several animal species and to investigate the effect of conventional antimicrobials on biofilm reduction. A total of 35 S. pseudintermedius and 192 CoNS were included. Biofilm formation was accessed by the microtiter plate assay and the biofilms were stained by crystal violet. Association between biofilm formation and staphylococci species and antimicrobial resistance was also performed. Biofilm susceptibility testing was performed with tetracycline and amikacin at the minimum inhibitory concentration (MIC) and 10 × MIC. The metabolic activity of the biofilm cells after antimicrobial treatment was accessed by the XTT assay. All isolates formed biofilm, with S. urealyticus producing the most biofilm biomass and S. pseudintermedius producing the least biomass. There was a positive association between biofilm formation and multidrug resistance as well as resistance to individual antimicrobials. Neither tetracycline nor amikacin were able to eradicate the biofilm, not even at the highest concentration used. This study provides new insights into biofilm formation and the effects of antimicrobials on CoNS species.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSilva, VanessaCorreia, ElisetePereira, José EduardoGonzález-Machado, CaminoCapita, RosaAlonso-Calleja, CarlosIgrejas, GilbertoPoeta, Patrícia2022-09-06T22:50:19Z2022-06-162022-06-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/143555eng2076-0817PURE: 46301814https://doi.org/10.3390/pathogens11060689info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:03Zoai:run.unl.pt:10362/143555Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:00.808143Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
spellingShingle Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
Silva, Vanessa
biofilm
coagulase-negative staphylococci (CoNS)
staphylococci
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_full Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_fullStr Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_full_unstemmed Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_sort Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
author Silva, Vanessa
author_facet Silva, Vanessa
Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author_role author
author2 Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Silva, Vanessa
Correia, Elisete
Pereira, José Eduardo
González-Machado, Camino
Capita, Rosa
Alonso-Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
dc.subject.por.fl_str_mv biofilm
coagulase-negative staphylococci (CoNS)
staphylococci
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic biofilm
coagulase-negative staphylococci (CoNS)
staphylococci
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description NORTE-01-0145-FEDER-030101 UIDB/CVT/00772/2020 LA/P/0059/2020 RTI2018-098267-R-C33 LE018P20
publishDate 2022
dc.date.none.fl_str_mv 2022-09-06T22:50:19Z
2022-06-16
2022-06-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143555
url http://hdl.handle.net/10362/143555
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-0817
PURE: 46301814
https://doi.org/10.3390/pathogens11060689
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138105386598400

Registros relacionados