Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Pereira, José Eduardo, Maltez, Luís, Poeta, Patrícia, Igrejas, Gilberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/150650
Resumo: This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors.
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spelling Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Waterbiofilmscoagulase-negative staphylococcienvironmentS. aureusStaphylococcusImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingThis work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors.The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSilva, VanessaPereira, José EduardoMaltez, LuísPoeta, PatríciaIgrejas, Gilberto2023-03-15T22:35:08Z2022-09-202022-09-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/150650engSilva, V., Pereira, J. E., Maltez, L., Poeta, P., & Igrejas, G. (2022). Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water. Pathogens, 11(10), [1069]. https://doi.org/10.3390/pathogens111010692076-0817PURE: 55918605https://doi.org/10.3390/pathogens11101069info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:32:47Zoai:run.unl.pt:10362/150650Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:14.355619Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
title Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
spellingShingle Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
Silva, Vanessa
biofilms
coagulase-negative staphylococci
environment
S. aureus
Staphylococcus
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
title_full Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
title_fullStr Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
title_full_unstemmed Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
title_sort Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
author Silva, Vanessa
author_facet Silva, Vanessa
Pereira, José Eduardo
Maltez, Luís
Poeta, Patrícia
Igrejas, Gilberto
author_role author
author2 Pereira, José Eduardo
Maltez, Luís
Poeta, Patrícia
Igrejas, Gilberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Silva, Vanessa
Pereira, José Eduardo
Maltez, Luís
Poeta, Patrícia
Igrejas, Gilberto
dc.subject.por.fl_str_mv biofilms
coagulase-negative staphylococci
environment
S. aureus
Staphylococcus
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
topic biofilms
coagulase-negative staphylococci
environment
S. aureus
Staphylococcus
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
description This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-20
2022-09-20T00:00:00Z
2023-03-15T22:35:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/150650
url http://hdl.handle.net/10362/150650
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, V., Pereira, J. E., Maltez, L., Poeta, P., & Igrejas, G. (2022). Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water. Pathogens, 11(10), [1069]. https://doi.org/10.3390/pathogens11101069
2076-0817
PURE: 55918605
https://doi.org/10.3390/pathogens11101069
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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