Development of disposable biosensor for the detection of breast cancer antigen 15-3

• Autor(a) principal: Cardoso, Helena Beatriz Gomes
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/28069
• topic: Biosensor; Breats cancer; Graphene; ELISA; Field effect transistors

This work describes the development of graphene electrochemical biosensors for the detection of CA15-3 protein, a recognized breast cancer tumor marker, using graphene-based field effect transistors (Gr-FET) functionalized with a specific antibody for this protein. The calibration model provided a correlation coefficient of 0.9959 and a detection limit of de 0.07 U mL-1. The recovery values obtained demonstrate its suitability for the quantification of CA15-3 protein in standard solutions. The ELISA method was also used as a comparison method for biosensor validation. Additionally, the feasibility of using the biosensor developed for detection and quantification of this marker in saliva samples was tested, aiming at the development of a low cost, disposable and fast technology that allows quantification using noninvasive methods, illustrating the potential of this method in the diagnosis of a highly prevalent disease.