Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source

Detalhes bibliográficos
Autor(a) principal: Concórdio-Reis, Patrícia
Data de Publicação: 2023
Outros Autores: Serafim, Bruno, Pereira, João R., Moppert, Xavier, Guézennec, Jean, Reis, Maria A. M., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158386
Resumo: project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB. Publisher Copyright: © 2023 The Author(s)
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spelling Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon sourceAlteromonas macleodii Mo169Apple pulp wasteBlue biotechnologyExopolysaccharide (EPS)Environmental Science(all)Soil SciencePlant ScienceSDG 14 - Life Below Waterproject LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB. Publisher Copyright: © 2023 The Author(s)A sugar-rich apple pulp waste generated from fruit processing for juice production was used as the sole carbon source for the cultivation of Alteromonas macleodii Mo169, a marine bacterium known for its EPS-secreting ability. The strain efficiently utilized the glucose and fructose present in the apple pulp waste, reaching biomass and EPS production of 9.20 ± 0.61 and 3.51 ± 0.08 g L−1, respectively, in 24-hour bioreactor cultivation. Two high molecular weight (Mw) fractions (1.7 ± 0.0 and 0.74 ± 0.0 MDa) were detected in the sample recovered from the cell-free supernatant by dialysis. The compositional analysis revealed the presence of glucose (31.1 ± 0.2 mol%), arabinose (23.9 ± 0.1 mol%), mannose (17.3 ± 0.1 mol%), glucosamine (10.3 ± 0.5 mol%), galactose (8.7 ± 0.0 mol%) and galacturonic acid (8.7 ± 0.0 mol%), as well as a high content in sulphate (6.0 ± 0.5 wt%). Given the presence of a high Mw polysaccharide in the apple pulp waste, probably pectin, a fraction of the detected sugar monomers might be attributed to that polymer, which was recovered together with A. macleodii Mo169 EPS. Concomitant with EPS synthesis, there was a viscosity build-up in the cultivation broth, which developed a shear-thinning fluid behaviour not observed in the initial medium. Therefore, this study demonstrates that apple pulp waste can be efficiently converted into a novel polysaccharide by A. macleodii Mo169 in a sustainable bioprocess. Moreover, the EPS sugar and acyl composition, together with its good thickening capacity, render the biopolymer of interest for use in several applications.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNConcórdio-Reis, PatríciaSerafim, BrunoPereira, João R.Moppert, XavierGuézennec, JeanReis, Maria A. M.Freitas, Filomena2023-09-27T22:26:17Z2023-052023-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/158386eng2352-1864PURE: 72591301https://doi.org/10.1016/j.eti.2023.103090info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:44Zoai:run.unl.pt:10362/158386Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:06.680258Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
title Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
spellingShingle Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
Concórdio-Reis, Patrícia
Alteromonas macleodii Mo169
Apple pulp waste
Blue biotechnology
Exopolysaccharide (EPS)
Environmental Science(all)
Soil Science
Plant Science
SDG 14 - Life Below Water
title_short Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
title_full Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
title_fullStr Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
title_full_unstemmed Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
title_sort Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
author Concórdio-Reis, Patrícia
author_facet Concórdio-Reis, Patrícia
Serafim, Bruno
Pereira, João R.
Moppert, Xavier
Guézennec, Jean
Reis, Maria A. M.
Freitas, Filomena
author_role author
author2 Serafim, Bruno
Pereira, João R.
Moppert, Xavier
Guézennec, Jean
Reis, Maria A. M.
Freitas, Filomena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Concórdio-Reis, Patrícia
Serafim, Bruno
Pereira, João R.
Moppert, Xavier
Guézennec, Jean
Reis, Maria A. M.
Freitas, Filomena
dc.subject.por.fl_str_mv Alteromonas macleodii Mo169
Apple pulp waste
Blue biotechnology
Exopolysaccharide (EPS)
Environmental Science(all)
Soil Science
Plant Science
SDG 14 - Life Below Water
topic Alteromonas macleodii Mo169
Apple pulp waste
Blue biotechnology
Exopolysaccharide (EPS)
Environmental Science(all)
Soil Science
Plant Science
SDG 14 - Life Below Water
description project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB. Publisher Copyright: © 2023 The Author(s)
publishDate 2023
dc.date.none.fl_str_mv 2023-09-27T22:26:17Z
2023-05
2023-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158386
url http://hdl.handle.net/10362/158386
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2352-1864
PURE: 72591301
https://doi.org/10.1016/j.eti.2023.103090
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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