Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide

Detalhes bibliográficos
Autor(a) principal: Concórdio-Reis, Patrícia
Data de Publicação: 2023
Outros Autores: Macedo, Ana Catarina, Cardeira, Martim, Moppert, Xavier, Guézennec, Jean, Sevrin, Chantal, Grandfils, Christian, Serra, Ana Teresa, Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158337
Resumo: Funding Information: This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledges FCT/MCTES for the Individual Grant CEEC-IND/04801/2017. Publisher Copyright: © 2023 by the authors.
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spelling Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 ExopolysaccharideSynthesis, Characterization, and In Vitro Antioxidant ActivityAlteromonas macleodii Mo 169bioactive materialblue biotechnologyexopolysaccharide (EPS)selenium nanoparticles (SeNP)BioengineeringSDG 14 - Life Below WaterFunding Information: This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledges FCT/MCTES for the Individual Grant CEEC-IND/04801/2017. Publisher Copyright: © 2023 by the authors.In this study, the novel exopolysaccharide (EPS) produced by the marine bacterium Alteromonas macleodii Mo 169 was used as a stabilizer and capping agent in the preparation of selenium nanoparticles (SeNPs). The synthesized nanoparticles were well dispersed and spherical with an average particle size of 32 nm. The cytotoxicity of the EPS and the EPS/SeNPs bio-nanocomposite was investigated on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines. No cytotoxicity was found for the EPS alone for concentrations up to 1 g L−1. A cytotoxic effect was only noticed for the bio-nanocomposite at the highest concentrations tested (0.5 and 1 g L−1). In vitro experiments demonstrated that non-cytotoxic concentrations of the EPS/SeNPs bio-nanocomposite had a significant cellular antioxidant effect on the HaCaT cell line by reducing ROS levels up to 33.8%. These findings demonstrated that the A. macleodii Mo 169 EPS can be efficiently used as a stabilizer and surface coating to produce a SeNP-based bio-nanocomposite with improved antioxidant activity.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNConcórdio-Reis, PatríciaMacedo, Ana CatarinaCardeira, MartimMoppert, XavierGuézennec, JeanSevrin, ChantalGrandfils, ChristianSerra, Ana TeresaFreitas, Filomena2023-09-26T22:21:44Z2023-02-022023-02-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://hdl.handle.net/10362/158337eng2306-5354PURE: 72484442https://doi.org/10.3390/bioengineering10020193info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:41Zoai:run.unl.pt:10362/158337Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:05.061577Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
Synthesis, Characterization, and In Vitro Antioxidant Activity
title Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
spellingShingle Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
Concórdio-Reis, Patrícia
Alteromonas macleodii Mo 169
bioactive material
blue biotechnology
exopolysaccharide (EPS)
selenium nanoparticles (SeNP)
Bioengineering
SDG 14 - Life Below Water
title_short Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
title_full Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
title_fullStr Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
title_full_unstemmed Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
title_sort Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
author Concórdio-Reis, Patrícia
author_facet Concórdio-Reis, Patrícia
Macedo, Ana Catarina
Cardeira, Martim
Moppert, Xavier
Guézennec, Jean
Sevrin, Chantal
Grandfils, Christian
Serra, Ana Teresa
Freitas, Filomena
author_role author
author2 Macedo, Ana Catarina
Cardeira, Martim
Moppert, Xavier
Guézennec, Jean
Sevrin, Chantal
Grandfils, Christian
Serra, Ana Teresa
Freitas, Filomena
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Concórdio-Reis, Patrícia
Macedo, Ana Catarina
Cardeira, Martim
Moppert, Xavier
Guézennec, Jean
Sevrin, Chantal
Grandfils, Christian
Serra, Ana Teresa
Freitas, Filomena
dc.subject.por.fl_str_mv Alteromonas macleodii Mo 169
bioactive material
blue biotechnology
exopolysaccharide (EPS)
selenium nanoparticles (SeNP)
Bioengineering
SDG 14 - Life Below Water
topic Alteromonas macleodii Mo 169
bioactive material
blue biotechnology
exopolysaccharide (EPS)
selenium nanoparticles (SeNP)
Bioengineering
SDG 14 - Life Below Water
description Funding Information: This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledges FCT/MCTES for the Individual Grant CEEC-IND/04801/2017. Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-26T22:21:44Z
2023-02-02
2023-02-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158337
url http://hdl.handle.net/10362/158337
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2306-5354
PURE: 72484442
https://doi.org/10.3390/bioengineering10020193
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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