From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.8/4208 |
Resumo: | This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the projects UID/MAR/04292/2019, ENVIRONOME (PTDC/AGR-PRO/3496/2012 - POCI-01-0145-FEDER016773), and grants awarded to TS (SFRH/BD/98266/2013), SN (SFRH/BPD/94500/2013), and TNL (SFRH/BPD/110943/2015). Project was also partially funded by the Integrated Programme of SR& TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. DR was also supported by EU FP7 Sustainable Nanotechnologies Project (SUN, contract number 604305). The authors also acknowledge Luís Alves and Sofia Silva for the assistance in the experimental procedure. |
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From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulationBiomarkersChlorothalonilCollembolaIn situ validationFungicidesqPCRThis work was supported by the Portuguese Foundation for Science and Technology (FCT) through the projects UID/MAR/04292/2019, ENVIRONOME (PTDC/AGR-PRO/3496/2012 - POCI-01-0145-FEDER016773), and grants awarded to TS (SFRH/BD/98266/2013), SN (SFRH/BPD/94500/2013), and TNL (SFRH/BPD/110943/2015). Project was also partially funded by the Integrated Programme of SR& TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. DR was also supported by EU FP7 Sustainable Nanotechnologies Project (SUN, contract number 604305). The authors also acknowledge Luís Alves and Sofia Silva for the assistance in the experimental procedure.Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg−1 ) and half this concentration (60 mg a.i. kg−1 ). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.IC-OnlineSimões, TiagoNovais, Sara C.Natal-da-Luz, TiagoRenaud, MathieuLeston, SaraRamos, FernandoRömbke, JörgDick, RoelofsVan Straalen, Nico M.Sousa, José P.Lemos, Marco F. L.2019-10-15T16:03:00Z2019-062019-10-07T10:40:31Z2019-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4208engSimões, T., Novais, S.C., Natal-da-Luz, T., Renaud, M., Leston, S., Ramos, F., Römbke, J., Roelofs, D., van Straalen, N.M., Sousa, J.P., Lemos, M.F.L. (2019). From laboratory to the field: validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation. Environment International 127, 522-530.0160-4120cv-prod-56640710.1016/j.envint.2019.03.073metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:48Zoai:iconline.ipleiria.pt:10400.8/4208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:07.431978Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| title |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| spellingShingle |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation Simões, Tiago Biomarkers Chlorothalonil Collembola In situ validation Fungicides qPCR |
| title_short |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| title_full |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| title_fullStr |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| title_full_unstemmed |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| title_sort |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
| author |
Simões, Tiago |
| author_facet |
Simões, Tiago Novais, Sara C. Natal-da-Luz, Tiago Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Dick, Roelofs Van Straalen, Nico M. Sousa, José P. Lemos, Marco F. L. |
| author_role |
author |
| author2 |
Novais, Sara C. Natal-da-Luz, Tiago Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Dick, Roelofs Van Straalen, Nico M. Sousa, José P. Lemos, Marco F. L. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
IC-Online |
| dc.contributor.author.fl_str_mv |
Simões, Tiago Novais, Sara C. Natal-da-Luz, Tiago Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Dick, Roelofs Van Straalen, Nico M. Sousa, José P. Lemos, Marco F. L. |
| dc.subject.por.fl_str_mv |
Biomarkers Chlorothalonil Collembola In situ validation Fungicides qPCR |
| topic |
Biomarkers Chlorothalonil Collembola In situ validation Fungicides qPCR |
| description |
This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the projects UID/MAR/04292/2019, ENVIRONOME (PTDC/AGR-PRO/3496/2012 - POCI-01-0145-FEDER016773), and grants awarded to TS (SFRH/BD/98266/2013), SN (SFRH/BPD/94500/2013), and TNL (SFRH/BPD/110943/2015). Project was also partially funded by the Integrated Programme of SR& TD “SmartBioR” (reference Centro-01-0145-FEDER-000018) cofunded by Centro 2020 program, Portugal2020, European Union, through the European Regional Development Fund. DR was also supported by EU FP7 Sustainable Nanotechnologies Project (SUN, contract number 604305). The authors also acknowledge Luís Alves and Sofia Silva for the assistance in the experimental procedure. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-10-15T16:03:00Z 2019-06 2019-10-07T10:40:31Z 2019-06-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/4208 |
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http://hdl.handle.net/10400.8/4208 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Simões, T., Novais, S.C., Natal-da-Luz, T., Renaud, M., Leston, S., Ramos, F., Römbke, J., Roelofs, D., van Straalen, N.M., Sousa, J.P., Lemos, M.F.L. (2019). From laboratory to the field: validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation. Environment International 127, 522-530. 0160-4120 cv-prod-566407 10.1016/j.envint.2019.03.073 |
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metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
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openAccess |
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application/pdf |
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