From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation

Detalhes bibliográficos
Autor(a) principal: Simões, Tiago
Data de Publicação: 2019
Outros Autores: Novais, Sara C, Luz, Tiago Natal da, Renaud, Mathieu, Leston, Sara, Ramos, Fernando, Römbke, Jörg, Roelofs, Dick, van Straalen, Nico M, Sousa, José P., Lemos, Marco Filipe Loureiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/101620
https://doi.org/10.1016/j.envint.2019.03.073
Resumo: Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.
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spelling From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulationBiomarkersChlorothalonilCollembolaFungicidesIn situ validationqPCRAnimalsArthropodsBiomarkersFungicides, IndustrialLaboratoriesNitrilesPesticidesReproductionSoilUnder controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/101620http://hdl.handle.net/10316/101620https://doi.org/10.1016/j.envint.2019.03.073eng01604120Simões, TiagoNovais, Sara CLuz, Tiago Natal daRenaud, MathieuLeston, SaraRamos, FernandoRömbke, JörgRoelofs, Dickvan Straalen, Nico MSousa, José P.Lemos, Marco Filipe Loureiroinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T20:43:25Zoai:estudogeral.uc.pt:10316/101620Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:18:46.538447Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
title From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
spellingShingle From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
Simões, Tiago
Biomarkers
Chlorothalonil
Collembola
Fungicides
In situ validation
qPCR
Animals
Arthropods
Biomarkers
Fungicides, Industrial
Laboratories
Nitriles
Pesticides
Reproduction
Soil
title_short From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
title_full From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
title_fullStr From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
title_full_unstemmed From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
title_sort From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
author Simões, Tiago
author_facet Simões, Tiago
Novais, Sara C
Luz, Tiago Natal da
Renaud, Mathieu
Leston, Sara
Ramos, Fernando
Römbke, Jörg
Roelofs, Dick
van Straalen, Nico M
Sousa, José P.
Lemos, Marco Filipe Loureiro
author_role author
author2 Novais, Sara C
Luz, Tiago Natal da
Renaud, Mathieu
Leston, Sara
Ramos, Fernando
Römbke, Jörg
Roelofs, Dick
van Straalen, Nico M
Sousa, José P.
Lemos, Marco Filipe Loureiro
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Simões, Tiago
Novais, Sara C
Luz, Tiago Natal da
Renaud, Mathieu
Leston, Sara
Ramos, Fernando
Römbke, Jörg
Roelofs, Dick
van Straalen, Nico M
Sousa, José P.
Lemos, Marco Filipe Loureiro
dc.subject.por.fl_str_mv Biomarkers
Chlorothalonil
Collembola
Fungicides
In situ validation
qPCR
Animals
Arthropods
Biomarkers
Fungicides, Industrial
Laboratories
Nitriles
Pesticides
Reproduction
Soil
topic Biomarkers
Chlorothalonil
Collembola
Fungicides
In situ validation
qPCR
Animals
Arthropods
Biomarkers
Fungicides, Industrial
Laboratories
Nitriles
Pesticides
Reproduction
Soil
description Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/101620
http://hdl.handle.net/10316/101620
https://doi.org/10.1016/j.envint.2019.03.073
url http://hdl.handle.net/10316/101620
https://doi.org/10.1016/j.envint.2019.03.073
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 01604120
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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