Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11800 |
Resumo: | In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched. |
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Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortiaGradient gel-electrophoresisComplete nucleotide-sequenceHost-range plasmidsBacterial communitiesAcinetobacter-calcoaceticusPseudomonas-AeruginosaMicrobial communitiesMultivariate analysesResistance plasmidsDioxygenase genesIn this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.Deutsche Forschungsgemeinschaft [SM59/4-1, 4-2]; FAPERJ-Brazil; European Commission [003998, 211684]; Alexander-von-Humboldt-Stiftung; CONICET (Argentina)Wiley-Blackwell Publishing, IncSapientiaGomes, Newton C. M.Flocco, Cecilia G.Costa, RodrigoJunca, HowardVilchez, RamiroPieper, Dietmar H.Kroegerrecklenfort, EllenParanhos, RodolfoMendonca-Hagler, Leda C. S.Smalla, Kornelia2018-12-07T14:57:59Z2010-112010-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11800eng0168-649610.1111/j.1574-6941.2010.00962.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:39Zoai:sapientia.ualg.pt:10400.1/11800Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:15.266770Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
title |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
spellingShingle |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia Gomes, Newton C. M. Gradient gel-electrophoresis Complete nucleotide-sequence Host-range plasmids Bacterial communities Acinetobacter-calcoaceticus Pseudomonas-Aeruginosa Microbial communities Multivariate analyses Resistance plasmids Dioxygenase genes |
title_short |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
title_full |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
title_fullStr |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
title_full_unstemmed |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
title_sort |
Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia |
author |
Gomes, Newton C. M. |
author_facet |
Gomes, Newton C. M. Flocco, Cecilia G. Costa, Rodrigo Junca, Howard Vilchez, Ramiro Pieper, Dietmar H. Kroegerrecklenfort, Ellen Paranhos, Rodolfo Mendonca-Hagler, Leda C. S. Smalla, Kornelia |
author_role |
author |
author2 |
Flocco, Cecilia G. Costa, Rodrigo Junca, Howard Vilchez, Ramiro Pieper, Dietmar H. Kroegerrecklenfort, Ellen Paranhos, Rodolfo Mendonca-Hagler, Leda C. S. Smalla, Kornelia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Gomes, Newton C. M. Flocco, Cecilia G. Costa, Rodrigo Junca, Howard Vilchez, Ramiro Pieper, Dietmar H. Kroegerrecklenfort, Ellen Paranhos, Rodolfo Mendonca-Hagler, Leda C. S. Smalla, Kornelia |
dc.subject.por.fl_str_mv |
Gradient gel-electrophoresis Complete nucleotide-sequence Host-range plasmids Bacterial communities Acinetobacter-calcoaceticus Pseudomonas-Aeruginosa Microbial communities Multivariate analyses Resistance plasmids Dioxygenase genes |
topic |
Gradient gel-electrophoresis Complete nucleotide-sequence Host-range plasmids Bacterial communities Acinetobacter-calcoaceticus Pseudomonas-Aeruginosa Microbial communities Multivariate analyses Resistance plasmids Dioxygenase genes |
description |
In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11 2010-11-01T00:00:00Z 2018-12-07T14:57:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11800 |
url |
http://hdl.handle.net/10400.1/11800 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0168-6496 10.1111/j.1574-6941.2010.00962.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley-Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133267065044992 |