Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia

Detalhes bibliográficos
Autor(a) principal: Gomes, Newton C. M.
Data de Publicação: 2010
Outros Autores: Flocco, Cecilia G., Costa, Rodrigo, Junca, Howard, Vilchez, Ramiro, Pieper, Dietmar H., Kroegerrecklenfort, Ellen, Paranhos, Rodolfo, Mendonca-Hagler, Leda C. S., Smalla, Kornelia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/11800
Resumo: In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.
id RCAP_18ab298fd21ac9006951025476b51fe1
oai_identifier_str oai:sapientia.ualg.pt:10400.1/11800
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortiaGradient gel-electrophoresisComplete nucleotide-sequenceHost-range plasmidsBacterial communitiesAcinetobacter-calcoaceticusPseudomonas-AeruginosaMicrobial communitiesMultivariate analysesResistance plasmidsDioxygenase genesIn this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.Deutsche Forschungsgemeinschaft [SM59/4-1, 4-2]; FAPERJ-Brazil; European Commission [003998, 211684]; Alexander-von-Humboldt-Stiftung; CONICET (Argentina)Wiley-Blackwell Publishing, IncSapientiaGomes, Newton C. M.Flocco, Cecilia G.Costa, RodrigoJunca, HowardVilchez, RamiroPieper, Dietmar H.Kroegerrecklenfort, EllenParanhos, RodolfoMendonca-Hagler, Leda C. S.Smalla, Kornelia2018-12-07T14:57:59Z2010-112010-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11800eng0168-649610.1111/j.1574-6941.2010.00962.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:39Zoai:sapientia.ualg.pt:10400.1/11800Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:15.266770Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
title Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
spellingShingle Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
Gomes, Newton C. M.
Gradient gel-electrophoresis
Complete nucleotide-sequence
Host-range plasmids
Bacterial communities
Acinetobacter-calcoaceticus
Pseudomonas-Aeruginosa
Microbial communities
Multivariate analyses
Resistance plasmids
Dioxygenase genes
title_short Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
title_full Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
title_fullStr Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
title_full_unstemmed Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
title_sort Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia
author Gomes, Newton C. M.
author_facet Gomes, Newton C. M.
Flocco, Cecilia G.
Costa, Rodrigo
Junca, Howard
Vilchez, Ramiro
Pieper, Dietmar H.
Kroegerrecklenfort, Ellen
Paranhos, Rodolfo
Mendonca-Hagler, Leda C. S.
Smalla, Kornelia
author_role author
author2 Flocco, Cecilia G.
Costa, Rodrigo
Junca, Howard
Vilchez, Ramiro
Pieper, Dietmar H.
Kroegerrecklenfort, Ellen
Paranhos, Rodolfo
Mendonca-Hagler, Leda C. S.
Smalla, Kornelia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Gomes, Newton C. M.
Flocco, Cecilia G.
Costa, Rodrigo
Junca, Howard
Vilchez, Ramiro
Pieper, Dietmar H.
Kroegerrecklenfort, Ellen
Paranhos, Rodolfo
Mendonca-Hagler, Leda C. S.
Smalla, Kornelia
dc.subject.por.fl_str_mv Gradient gel-electrophoresis
Complete nucleotide-sequence
Host-range plasmids
Bacterial communities
Acinetobacter-calcoaceticus
Pseudomonas-Aeruginosa
Microbial communities
Multivariate analyses
Resistance plasmids
Dioxygenase genes
topic Gradient gel-electrophoresis
Complete nucleotide-sequence
Host-range plasmids
Bacterial communities
Acinetobacter-calcoaceticus
Pseudomonas-Aeruginosa
Microbial communities
Multivariate analyses
Resistance plasmids
Dioxygenase genes
description In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches (rhizospheres and bulk sediment). In addition, we aimed to recover PH-degrading consortia (PHDC) for future use in remediation strategies. The PHDC were enriched with petroleum from rhizosphere and bulk sediment samples taken from a mangrove chronically polluted with oil hydrocarbons. Southern blot hybridization (SBH) assays of PCR amplicons from environmental DNA before enrichments resulted in weak positive signals for the functional gene types targeted, suggesting that PH-degrading genotypes and plasmids were in low abundance in the rhizosphere and bulk sediments. However, after enrichment, these genes were detected and strong microniche-dependent differences in the abundance and composition of hydrocarbonoclastic bacterial populations, plasmids (IncP-1 alpha, IncP-1 beta, IncP-7 and IncP-9) and functional genes (naphthalene, extradiol and intradiol dioxygenases) were revealed by in-depth molecular analyses [PCR-denaturing gradient gel electrophoresis and hybridization (SBH and microarray)]. Our results suggest that, despite the low abundance of PH-degrading genes and plasmids in the environmental samples, the original bacterial composition of the mangrove microniches determined the structural and functional diversity of the PHDC enriched.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
2010-11-01T00:00:00Z
2018-12-07T14:57:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/11800
url http://hdl.handle.net/10400.1/11800
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0168-6496
10.1111/j.1574-6941.2010.00962.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley-Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133267065044992