Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study

Detalhes bibliográficos
Autor(a) principal: Silva-Correia, Joana
Data de Publicação: 2013
Outros Autores: Zavan, B., Vindigni, V., Silva, Tiago H., Oliveira, Joaquim M., Abatangelo, G., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23665
Resumo: In this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo.
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spelling Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo studyBiocompatibilityHydrogelsIn vitroIn vivoMethacrylated gellan gumScience & TechnologyIn this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo.The authors thank the funds provided by Portuguese Foundation for Science and Technology (FCT) through POCTI and FEDER programmes. This work was also supported by the European Union funded Collaborative Project Disc Regeneration (NMP3-LA-2008-213904).WileyUniversidade do MinhoSilva-Correia, JoanaZavan, B.Vindigni, V.Silva, Tiago H.Oliveira, Joaquim M.Abatangelo, G.Reis, R. L.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23665eng2192-265910.1002/adhm.20120025623184642http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200256/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:04Zoai:repositorium.sdum.uminho.pt:1822/23665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:15.046052Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
title Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
spellingShingle Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
Silva-Correia, Joana
Biocompatibility
Hydrogels
In vitro
In vivo
Methacrylated gellan gum
Science & Technology
title_short Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
title_full Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
title_fullStr Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
title_full_unstemmed Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
title_sort Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
author Silva-Correia, Joana
author_facet Silva-Correia, Joana
Zavan, B.
Vindigni, V.
Silva, Tiago H.
Oliveira, Joaquim M.
Abatangelo, G.
Reis, R. L.
author_role author
author2 Zavan, B.
Vindigni, V.
Silva, Tiago H.
Oliveira, Joaquim M.
Abatangelo, G.
Reis, R. L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva-Correia, Joana
Zavan, B.
Vindigni, V.
Silva, Tiago H.
Oliveira, Joaquim M.
Abatangelo, G.
Reis, R. L.
dc.subject.por.fl_str_mv Biocompatibility
Hydrogels
In vitro
In vivo
Methacrylated gellan gum
Science & Technology
topic Biocompatibility
Hydrogels
In vitro
In vivo
Methacrylated gellan gum
Science & Technology
description In this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23665
url http://hdl.handle.net/1822/23665
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2192-2659
10.1002/adhm.201200256
23184642
http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200256/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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