Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/23665 |
Resumo: | In this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo. |
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Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo studyBiocompatibilityHydrogelsIn vitroIn vivoMethacrylated gellan gumScience & TechnologyIn this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo.The authors thank the funds provided by Portuguese Foundation for Science and Technology (FCT) through POCTI and FEDER programmes. This work was also supported by the European Union funded Collaborative Project Disc Regeneration (NMP3-LA-2008-213904).WileyUniversidade do MinhoSilva-Correia, JoanaZavan, B.Vindigni, V.Silva, Tiago H.Oliveira, Joaquim M.Abatangelo, G.Reis, R. L.20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23665eng2192-265910.1002/adhm.20120025623184642http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200256/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:04Zoai:repositorium.sdum.uminho.pt:1822/23665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:15.046052Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
title |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
spellingShingle |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study Silva-Correia, Joana Biocompatibility Hydrogels In vitro In vivo Methacrylated gellan gum Science & Technology |
title_short |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
title_full |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
title_fullStr |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
title_full_unstemmed |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
title_sort |
Biocompatibility evaluation of ionic- and photo-crosslinked methacrylated gellan gum hydrogels: in vitro and In vivo study |
author |
Silva-Correia, Joana |
author_facet |
Silva-Correia, Joana Zavan, B. Vindigni, V. Silva, Tiago H. Oliveira, Joaquim M. Abatangelo, G. Reis, R. L. |
author_role |
author |
author2 |
Zavan, B. Vindigni, V. Silva, Tiago H. Oliveira, Joaquim M. Abatangelo, G. Reis, R. L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva-Correia, Joana Zavan, B. Vindigni, V. Silva, Tiago H. Oliveira, Joaquim M. Abatangelo, G. Reis, R. L. |
dc.subject.por.fl_str_mv |
Biocompatibility Hydrogels In vitro In vivo Methacrylated gellan gum Science & Technology |
topic |
Biocompatibility Hydrogels In vitro In vivo Methacrylated gellan gum Science & Technology |
description |
In this study, the stability and biocompatibility of methacrylated gellan gum hydrogels, obtained either by ionic- (iGG-MA) or photo-crosslinking (phGGMA), were evaluated in vitro and in vivo. Size exclusion chromatography analysis of the methacrylated gellan gum (GG-MA) powder revealed that molecular weight is lower as compared to the non-modifi ed material, i.e., low acyl gellan gum. The water uptake and swelling of iGG-MA and phGGMA hydrogels were investigated in phosphate-buffered saline solution (pH 7.4). The biocompatibility of the hydrogels was fi rstly evaluated by producing cell-laden hydrogels. The in vitro cells encapsulation study showed that lung fi broblast cells (L929 cells) and human intervertebral disc (hIVD) cells are viable when cultured within both hydrogels, up to 21 days of culturing. The iGG-MA and phGG-MA hydrogels were also subcutaneously implanted in Lewis rats for 10 and 18 days. Tissue response to the hydrogels implantation was determined by histological analysis (haematoxylin-eosin staining). A thin fi brous capsule was observed around the implanted hydrogels. No necrosis, calcifi cation, and acute infl ammatory reaction were observed. The results presented in this study demonstrate that iGG-MA and phGG-MA hydrogels are stable in vitro and in vivo, support L929 and hIVD cells’ encapsulation and viability, and were found to be well-tolerated and non-toxic in vivo. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/23665 |
url |
http://hdl.handle.net/1822/23665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2192-2659 10.1002/adhm.201200256 23184642 http://onlinelibrary.wiley.com/doi/10.1002/adhm.201200256/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133030258835456 |