Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging

Detalhes bibliográficos
Autor(a) principal: Vieira, Sílvia
Data de Publicação: 2023
Outros Autores: Strymecka, Paulina, Stanaszek, Luiza, Silva-Correia, Joana, Drela, Katarzyna, Fiedorowicz, Michał, Malysz-Cymborska, Izabela, Janowski, Miroslaw, Reis, R. L., Lukomska, Barbara, Walczak, Piotr, Oliveira, Joaquim M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/83747
Resumo: This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.
id RCAP_db748c1f7218e3445cb2b3204709b4c4
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/83747
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imagingCell deliveryGellan GumInjectable hydrogelsMagnetic resonance imagingManganeseScience & TechnologyThis work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.Sílvia Vieira acknowledges the FCT Ph.D. scholarship (SFRH/BD/102710/2014). J. Miguel Oliveira and J. Silva-Correia acknowledge the FCT grants under the Investigator FCT program (IF/01285/2015 and IF/00115/2015, respectively). The authors also acknowledge the funds provided under the project NanoTech4ALS, funded under the EU FP7 M-ERA.NET program, and ESF (POWR.03.02.00-00-I028/17-00).MDPIUniversidade do MinhoVieira, SílviaStrymecka, PaulinaStanaszek, LuizaSilva-Correia, JoanaDrela, KatarzynaFiedorowicz, MichałMalysz-Cymborska, IzabelaJanowski, MiroslawReis, R. L.Lukomska, BarbaraWalczak, PiotrOliveira, Joaquim M.2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83747engVieira S., Strymecka P., Stanaszek L., Silva-Correia J., Drela K., Fiedorowicz M., Malysz-Cymborska I., Janowski M., Reis R. L., Lukomska B., Walczak P., Oliveira J. M. Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging, Bioengineering, Vol. 10, Issue 4, pp. 427, doi:10.3390/bioengineering10040427, 20232306-535410.3390/bioengineering10040427427https://www.mdpi.com/2306-5354/10/4/427info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:54Zoai:repositorium.sdum.uminho.pt:1822/83747Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:02.685605Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
title Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
spellingShingle Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
Vieira, Sílvia
Cell delivery
Gellan Gum
Injectable hydrogels
Magnetic resonance imaging
Manganese
Science & Technology
title_short Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
title_full Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
title_fullStr Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
title_full_unstemmed Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
title_sort Mn-based methacrylated gellan gum hydrogels for MRI-guided cell delivery and imaging
author Vieira, Sílvia
author_facet Vieira, Sílvia
Strymecka, Paulina
Stanaszek, Luiza
Silva-Correia, Joana
Drela, Katarzyna
Fiedorowicz, Michał
Malysz-Cymborska, Izabela
Janowski, Miroslaw
Reis, R. L.
Lukomska, Barbara
Walczak, Piotr
Oliveira, Joaquim M.
author_role author
author2 Strymecka, Paulina
Stanaszek, Luiza
Silva-Correia, Joana
Drela, Katarzyna
Fiedorowicz, Michał
Malysz-Cymborska, Izabela
Janowski, Miroslaw
Reis, R. L.
Lukomska, Barbara
Walczak, Piotr
Oliveira, Joaquim M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Vieira, Sílvia
Strymecka, Paulina
Stanaszek, Luiza
Silva-Correia, Joana
Drela, Katarzyna
Fiedorowicz, Michał
Malysz-Cymborska, Izabela
Janowski, Miroslaw
Reis, R. L.
Lukomska, Barbara
Walczak, Piotr
Oliveira, Joaquim M.
dc.subject.por.fl_str_mv Cell delivery
Gellan Gum
Injectable hydrogels
Magnetic resonance imaging
Manganese
Science & Technology
topic Cell delivery
Gellan Gum
Injectable hydrogels
Magnetic resonance imaging
Manganese
Science & Technology
description This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
2023-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/83747
url https://hdl.handle.net/1822/83747
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vieira S., Strymecka P., Stanaszek L., Silva-Correia J., Drela K., Fiedorowicz M., Malysz-Cymborska I., Janowski M., Reis R. L., Lukomska B., Walczak P., Oliveira J. M. Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging, Bioengineering, Vol. 10, Issue 4, pp. 427, doi:10.3390/bioengineering10040427, 2023
2306-5354
10.3390/bioengineering10040427
427
https://www.mdpi.com/2306-5354/10/4/427
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133027772661760

Registros relacionados