Whole-cell SYBR Green I assay for antimalarial activity assessment

Detalhes bibliográficos
Autor(a) principal: Machado, M
Data de Publicação: 2016
Outros Autores: Murtinheira, F, Lobo, E, Nogueira, F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdf
Resumo: Malaria parasite growth assessment assays using nucleic acid dye SYBR GreenI are frequently compromised by high background readings due to haemoglobin and detergents in lysis buffer. Here, we have assessed and further validated an intact-cell SYBR Green I-based fluorescence assay (Cell-MSF) for antimalarial activity assessment even of highly fluorescent compounds. Our modifications include: • The absence of detergents and cell debris renders a high dynamic range and z’>0.7 • Tested against fluorescent and non-fluorescent compounds • Without cell lysis, one centrifugation and uses transparent 96 well plates
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spelling Whole-cell SYBR Green I assay for antimalarial activity assessmentMalariaPlasmodiumAntimalarial activity assessmentSYBR Green IWhole-cell assayFluorescent compoundsBiochemistry, Genetics and Molecular Biology (miscellaneous)ParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingMalaria parasite growth assessment assays using nucleic acid dye SYBR GreenI are frequently compromised by high background readings due to haemoglobin and detergents in lysis buffer. Here, we have assessed and further validated an intact-cell SYBR Green I-based fluorescence assay (Cell-MSF) for antimalarial activity assessment even of highly fluorescent compounds. Our modifications include: • The absence of detergents and cell debris renders a high dynamic range and z’>0.7 • Tested against fluorescent and non-fluorescent compounds • Without cell lysis, one centrifugation and uses transparent 96 well platesInstituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNMachado, MMurtinheira, FLobo, ENogueira, F2018-05-11T22:07:00Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdfengPURE: 2457803https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:12Zoai:run.unl.pt:10362/36652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:37.065457Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Whole-cell SYBR Green I assay for antimalarial activity assessment
title Whole-cell SYBR Green I assay for antimalarial activity assessment
spellingShingle Whole-cell SYBR Green I assay for antimalarial activity assessment
Machado, M
Malaria
Plasmodium
Antimalarial activity assessment
SYBR Green I
Whole-cell assay
Fluorescent compounds
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Whole-cell SYBR Green I assay for antimalarial activity assessment
title_full Whole-cell SYBR Green I assay for antimalarial activity assessment
title_fullStr Whole-cell SYBR Green I assay for antimalarial activity assessment
title_full_unstemmed Whole-cell SYBR Green I assay for antimalarial activity assessment
title_sort Whole-cell SYBR Green I assay for antimalarial activity assessment
author Machado, M
author_facet Machado, M
Murtinheira, F
Lobo, E
Nogueira, F
author_role author
author2 Murtinheira, F
Lobo, E
Nogueira, F
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Machado, M
Murtinheira, F
Lobo, E
Nogueira, F
dc.subject.por.fl_str_mv Malaria
Plasmodium
Antimalarial activity assessment
SYBR Green I
Whole-cell assay
Fluorescent compounds
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Malaria
Plasmodium
Antimalarial activity assessment
SYBR Green I
Whole-cell assay
Fluorescent compounds
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
description Malaria parasite growth assessment assays using nucleic acid dye SYBR GreenI are frequently compromised by high background readings due to haemoglobin and detergents in lysis buffer. Here, we have assessed and further validated an intact-cell SYBR Green I-based fluorescence assay (Cell-MSF) for antimalarial activity assessment even of highly fluorescent compounds. Our modifications include: • The absence of detergents and cell debris renders a high dynamic range and z’>0.7 • Tested against fluorescent and non-fluorescent compounds • Without cell lysis, one centrifugation and uses transparent 96 well plates
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2018-05-11T22:07:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdf
url https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 2457803
https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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