Translocating the blood-brain barrier using electrostatics

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Marta M. B.
Data de Publicação: 2012
Outros Autores: Domingues, Marco M., Freire, João M., Santos, Nuno C., Castanho, Miguel A. R. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/7309
Resumo: Copyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
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spelling Translocating the blood-brain barrier using electrostaticsBlood-brain barrierDrug targetingBlood cellsCell surface chargeZeta-potentialCopyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.Mammalian cell membranes regulate homeostasis, protein activity, and cell signaling. The charge at the membrane surface has been correlated with these key events. Although mammalian cells are known to be slightly anionic, quantitative information on the membrane charge and the importance of electrostatic interactions in pharmacokinetics and pharmacodynamics remain elusive. Recently, we reported for the first time that brain endothelial cells (EC) are more negatively charged than human umbilical cord cells, using zeta-potential measurements by dynamic light scattering. Here, we hypothesize that anionicity is a key feature of the blood-brain barrier (BBB) and contributes to select which compounds cross into the brain. For the sake of comparison, we also studied the membrane surface charge of blood components—red blood cells (RBC), platelets, and peripheral blood mononuclear cells (PBMC).To further quantitatively correlate the negative zeta-potential values with membrane charge density, model membranes with different percentages of anionic lipids were also evaluated. From all the cells tested, brain cell membranes are the most anionic and those having their lipids mostly exposed, which explains why lipophilic cationic compounds are more prone to cross the blood-brain barrier.Fundação para a Ciência e Tecnologia — Ministério da Educação e Ciência (FCT-MEC, Portugal) is acknowledged for funding (including fellowships SFRH/BD/42158/2007 to Marta M.B. Ribeiro, SFRH/BD/41750/2007 to Marco M. Domingues and SFRH/BD/70423/2010 to João M. Freire) and project PTDC/QUI-BIQ/119509/2010. Marie Curie Industry-Academia Partnerships and Pathways (European Commission) is also acknowledged for funding (FP7-PEOPLE-2007-3-1-IAPP, Project 230654).Frontiers Research FoundationRepositório da Universidade de LisboaRibeiro, Marta M. B.Domingues, Marco M.Freire, João M.Santos, Nuno C.Castanho, Miguel A. R. B.2012-11-28T16:11:20Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/7309engFrontiers in Cellular Neuroscience October 2012 | Volume6 | Article44 | 1-71662-5102http://dx.doi.org/10.3389/fncel.2012.00044info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:50:23Zoai:repositorio.ul.pt:10451/7309Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:32:09.552356Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Translocating the blood-brain barrier using electrostatics
title Translocating the blood-brain barrier using electrostatics
spellingShingle Translocating the blood-brain barrier using electrostatics
Ribeiro, Marta M. B.
Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
title_short Translocating the blood-brain barrier using electrostatics
title_full Translocating the blood-brain barrier using electrostatics
title_fullStr Translocating the blood-brain barrier using electrostatics
title_full_unstemmed Translocating the blood-brain barrier using electrostatics
title_sort Translocating the blood-brain barrier using electrostatics
author Ribeiro, Marta M. B.
author_facet Ribeiro, Marta M. B.
Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
author_role author
author2 Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ribeiro, Marta M. B.
Domingues, Marco M.
Freire, João M.
Santos, Nuno C.
Castanho, Miguel A. R. B.
dc.subject.por.fl_str_mv Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
topic Blood-brain barrier
Drug targeting
Blood cells
Cell surface charge
Zeta-potential
description Copyright © 2012 Ribeiro,Domingues, Freire,Santos and Castanho. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-28T16:11:20Z
2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/7309
url http://hdl.handle.net/10451/7309
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Cellular Neuroscience October 2012 | Volume6 | Article44 | 1-7
1662-5102
http://dx.doi.org/10.3389/fncel.2012.00044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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