A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/32538 |
Resumo: | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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A new noncanonical anionic peptide that translocates a cellular blood-brain barrier modelCell-penetrating peptideBlood–brain barrierAnionic peptideDrug-delivery systems© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).The capacity to transport therapeutic molecules across the blood–brain barrier (BBB) represents a breakthrough in the development of tools for the treatment of many central nervous system (CNS)-associated diseases. The BBB, while being protective against infectious agents, hinders the brain uptake of many drugs. Hence, finding safe shuttles able to overcome the BBB is of utmost importance. Herein, we identify a new BBB-translocating peptide with unique properties. For years it was thought that cationic sequences were mandatory for a cell-penetrating peptide (CPP) to achieve cellular internalization. Despite being anionic at physiological pH, PepNeg (sequence (SGTQEEY) is an efficient BBB translocator that is able to carry a large cargo (27 kDa), while maintaining BBB integrity. In addition, PepNeg is able to use two distinct methods of translocation, energy-dependent and -independent, suggesting that direct penetration might occur when low concentrations of peptide are presented to cells. The discovery of this new anionic trans-BBB peptide allows the development of new delivery systems to the CNS and contributes to the need to rethink the role of electrostatic attraction in BBB-translocationThe authors thank the Portuguese Funding Agency, Fundação para a Ciência e a Tecnologia, FCT IP, for financial support (grants SFRH/BPD/94466/2013; PTDC/BBBNAN/1578/2014) and Marie Sklodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE), call 20-MSCA-RISE-2014 (grant agreement H20 644167—INPACT).MDPIRepositório da Universidade de LisboaNeves Coelho, SaraEleutério, Rute P.Enguita, Francisco J.Neves, VeraCastanho, Miguel A. R. B.2018-04-02T14:47:34Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/32538engMolecules 2017, 22, 17531420-3049doi:10.3390/molecules22101753info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:26:53Zoai:repositorio.ul.pt:10451/32538Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:47:49.020713Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| title |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| spellingShingle |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model Neves Coelho, Sara Cell-penetrating peptide Blood–brain barrier Anionic peptide Drug-delivery systems |
| title_short |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| title_full |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| title_fullStr |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| title_full_unstemmed |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| title_sort |
A new noncanonical anionic peptide that translocates a cellular blood-brain barrier model |
| author |
Neves Coelho, Sara |
| author_facet |
Neves Coelho, Sara Eleutério, Rute P. Enguita, Francisco J. Neves, Vera Castanho, Miguel A. R. B. |
| author_role |
author |
| author2 |
Eleutério, Rute P. Enguita, Francisco J. Neves, Vera Castanho, Miguel A. R. B. |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Neves Coelho, Sara Eleutério, Rute P. Enguita, Francisco J. Neves, Vera Castanho, Miguel A. R. B. |
| dc.subject.por.fl_str_mv |
Cell-penetrating peptide Blood–brain barrier Anionic peptide Drug-delivery systems |
| topic |
Cell-penetrating peptide Blood–brain barrier Anionic peptide Drug-delivery systems |
| description |
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2018-04-02T14:47:34Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/32538 |
| url |
http://hdl.handle.net/10451/32538 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Molecules 2017, 22, 1753 1420-3049 doi:10.3390/molecules22101753 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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