Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/14393 |
Resumo: | The Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications. |
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Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineeringLayer-by-layerMultilayerScaffoldsParticlesAgglomeratePolysaccharidesRegenerative medicineScience & TechnologyThe Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications.Fundo Europeu de Desenvolvimento Regional (FEDER) através do Programa Operacional de Cooperação Transfronteiriça Espanha Portugal 2007-2013 (POCTEP)Fundação para a Ciência e a Tecnologia (FCT)Mary Ann LiebertUniversidade do MinhoMiranda, Emanuel SáSilva, Tiago H.Reis, R. L.Mano, J. F.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/14393eng2152-494710.1089/ten.tea.2010.063521790302http://www.liebertonline.com/doi/full/10.1089/ten.tea.2010.0635info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:37Zoai:repositorium.sdum.uminho.pt:1822/14393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:53.159895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
title |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
spellingShingle |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering Miranda, Emanuel Sá Layer-by-layer Multilayer Scaffolds Particles Agglomerate Polysaccharides Regenerative medicine Science & Technology |
title_short |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
title_full |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
title_fullStr |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
title_full_unstemmed |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
title_sort |
Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering |
author |
Miranda, Emanuel Sá |
author_facet |
Miranda, Emanuel Sá Silva, Tiago H. Reis, R. L. Mano, J. F. |
author_role |
author |
author2 |
Silva, Tiago H. Reis, R. L. Mano, J. F. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Miranda, Emanuel Sá Silva, Tiago H. Reis, R. L. Mano, J. F. |
dc.subject.por.fl_str_mv |
Layer-by-layer Multilayer Scaffolds Particles Agglomerate Polysaccharides Regenerative medicine Science & Technology |
topic |
Layer-by-layer Multilayer Scaffolds Particles Agglomerate Polysaccharides Regenerative medicine Science & Technology |
description |
The Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/14393 |
url |
http://hdl.handle.net/1822/14393 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2152-4947 10.1089/ten.tea.2010.0635 21790302 http://www.liebertonline.com/doi/full/10.1089/ten.tea.2010.0635 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert |
publisher.none.fl_str_mv |
Mary Ann Liebert |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132659354435584 |