Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering

Detalhes bibliográficos
Autor(a) principal: Miranda, Emanuel Sá
Data de Publicação: 2011
Outros Autores: Silva, Tiago H., Reis, R. L., Mano, J. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/14393
Resumo: The Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications.
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spelling Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineeringLayer-by-layerMultilayerScaffoldsParticlesAgglomeratePolysaccharidesRegenerative medicineScience & TechnologyThe Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications.Fundo Europeu de Desenvolvimento Regional (FEDER) através do Programa Operacional de Cooperação Transfronteiriça Espanha Portugal 2007-2013 (POCTEP)Fundação para a Ciência e a Tecnologia (FCT)Mary Ann LiebertUniversidade do MinhoMiranda, Emanuel SáSilva, Tiago H.Reis, R. L.Mano, J. F.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/14393eng2152-494710.1089/ten.tea.2010.063521790302http://www.liebertonline.com/doi/full/10.1089/ten.tea.2010.0635info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:37Zoai:repositorium.sdum.uminho.pt:1822/14393Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:53.159895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
title Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
spellingShingle Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
Miranda, Emanuel Sá
Layer-by-layer
Multilayer
Scaffolds
Particles
Agglomerate
Polysaccharides
Regenerative medicine
Science & Technology
title_short Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
title_full Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
title_fullStr Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
title_full_unstemmed Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
title_sort Nanostructured natural-based polyelectrolyte multilayers to agglomerate chitosan particles into scaffolds for tissue engineering
author Miranda, Emanuel Sá
author_facet Miranda, Emanuel Sá
Silva, Tiago H.
Reis, R. L.
Mano, J. F.
author_role author
author2 Silva, Tiago H.
Reis, R. L.
Mano, J. F.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Miranda, Emanuel Sá
Silva, Tiago H.
Reis, R. L.
Mano, J. F.
dc.subject.por.fl_str_mv Layer-by-layer
Multilayer
Scaffolds
Particles
Agglomerate
Polysaccharides
Regenerative medicine
Science & Technology
topic Layer-by-layer
Multilayer
Scaffolds
Particles
Agglomerate
Polysaccharides
Regenerative medicine
Science & Technology
description The Layer-by-Layer (LbL) deposition technique is a self-assembly process that allows the coating of material's surface with nanostructured layers of polyelectrolytes, allowing to control several surface properties. This technique presents some advantages when compared with other thin film assembly techniques like having the possibility to coat surfaces with complex geometries in mild conditions or to incorporate active compounds. Tissue engineering involves typically the use of porous biodegradable scaffolds for the temporary support of cells. Such structures can be produced by agglomeration of micro-spheres that needs to be fixed into a three dimensional structure. In this work we suggest the use of LbL to promote such mechanical fixation in free-formed micro-spheres assemblies and simultaneously to control the properties of its surface. For the proof of concept the biological performance of chitosan/alginate multilayers is first investigated in two-dimensional models in which the attachment and proliferation of L929 and ATDC5 cells are studied in function of the number of layers and the nature of the final layer. Scaffolds prepared by agglomeration of chitosan particles using the same multilayered system were processed and characterized; it was found that they could support the attachment and proliferation of ATDC5 cells. This study suggests that LbL can be used as a versatile methodology to prepare scaffolds by particle agglomeration that could be suitable for tissue engineering applications.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/14393
url http://hdl.handle.net/1822/14393
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2152-4947
10.1089/ten.tea.2010.0635
21790302
http://www.liebertonline.com/doi/full/10.1089/ten.tea.2010.0635
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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