Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/44377 |
Resumo: | Tissue engineering approaches for the development of a single epidermal-dermal scaffold to treat full-thickness skin defects have been limited by difficulties in the fabrication of a bilayer scaffold combining the specific properties of the epidermis and the dermis. Here we present an innovative approach to developing a scaffold that holds promise for skin tissue engineering. We utilize the spray-assisted layer-by-layer assembly technique to deposit a polyelectrolyte multilayer film composed of hyaluronic acid and poly-L-lysine (the epidermal component) on a porous hyaluronic acid scaffold (the dermal component), in a rapid and controlled manner. The multilayer film promotes cell adhesion, contributing to regeneration of the epidermal barrier functions of skin. While human keratinocytes attached and proliferated on the coated porous scaffolds, they did not invade the porous dermal component, thus leaving room for seeding of relevant fibroblast cell types in this scaffold. This scaffold therefore holds promise for co-culture of different cells, which may be useful for treatment of full-thickness skin defects as well as other tissue engineering applications. |
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Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineeringSpray-assisted layer-by-layer assembly, pPolyelectrolyte multilayer filmsSkin tissue engineering,Hyaluronic acidspray-assisted layer-by-layer assemblyScience & TechnologyTissue engineering approaches for the development of a single epidermal-dermal scaffold to treat full-thickness skin defects have been limited by difficulties in the fabrication of a bilayer scaffold combining the specific properties of the epidermis and the dermis. Here we present an innovative approach to developing a scaffold that holds promise for skin tissue engineering. We utilize the spray-assisted layer-by-layer assembly technique to deposit a polyelectrolyte multilayer film composed of hyaluronic acid and poly-L-lysine (the epidermal component) on a porous hyaluronic acid scaffold (the dermal component), in a rapid and controlled manner. The multilayer film promotes cell adhesion, contributing to regeneration of the epidermal barrier functions of skin. While human keratinocytes attached and proliferated on the coated porous scaffolds, they did not invade the porous dermal component, thus leaving room for seeding of relevant fibroblast cell types in this scaffold. This scaffold therefore holds promise for co-culture of different cells, which may be useful for treatment of full-thickness skin defects as well as other tissue engineering applications.Contract grant sponsor: Portuguese Foundation for Science and Technology; contract grant number: BD/39396/2007Contract grant sponsors: MIT-Portugal Program; National Science Foundation Graduate Research FellowshipJohn Wiley and SonsUniversidade do MinhoMonteiro, I. P.Shukla, A.Marques, A. P.Reis, R. L.Hammond, P. T.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/44377engMonteiro I. P., Shukla A., Marques A. P., Reis R. L., Hammond P. T. Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH A, Vol. 103, Issue 1, pp. 330-340, doi:10.1002/jbm.a.35178, 2015.1552-496510.1002/jbm.a.3517824659574http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35178/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:42Zoai:repositorium.sdum.uminho.pt:1822/44377Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:15:02.830358Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
title |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
spellingShingle |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering Monteiro, I. P. Spray-assisted layer-by-layer assembly, p Polyelectrolyte multilayer films Skin tissue engineering, Hyaluronic acid spray-assisted layer-by-layer assembly Science & Technology |
title_short |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
title_full |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
title_fullStr |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
title_full_unstemmed |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
title_sort |
Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering |
author |
Monteiro, I. P. |
author_facet |
Monteiro, I. P. Shukla, A. Marques, A. P. Reis, R. L. Hammond, P. T. |
author_role |
author |
author2 |
Shukla, A. Marques, A. P. Reis, R. L. Hammond, P. T. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Monteiro, I. P. Shukla, A. Marques, A. P. Reis, R. L. Hammond, P. T. |
dc.subject.por.fl_str_mv |
Spray-assisted layer-by-layer assembly, p Polyelectrolyte multilayer films Skin tissue engineering, Hyaluronic acid spray-assisted layer-by-layer assembly Science & Technology |
topic |
Spray-assisted layer-by-layer assembly, p Polyelectrolyte multilayer films Skin tissue engineering, Hyaluronic acid spray-assisted layer-by-layer assembly Science & Technology |
description |
Tissue engineering approaches for the development of a single epidermal-dermal scaffold to treat full-thickness skin defects have been limited by difficulties in the fabrication of a bilayer scaffold combining the specific properties of the epidermis and the dermis. Here we present an innovative approach to developing a scaffold that holds promise for skin tissue engineering. We utilize the spray-assisted layer-by-layer assembly technique to deposit a polyelectrolyte multilayer film composed of hyaluronic acid and poly-L-lysine (the epidermal component) on a porous hyaluronic acid scaffold (the dermal component), in a rapid and controlled manner. The multilayer film promotes cell adhesion, contributing to regeneration of the epidermal barrier functions of skin. While human keratinocytes attached and proliferated on the coated porous scaffolds, they did not invade the porous dermal component, thus leaving room for seeding of relevant fibroblast cell types in this scaffold. This scaffold therefore holds promise for co-culture of different cells, which may be useful for treatment of full-thickness skin defects as well as other tissue engineering applications. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/44377 |
url |
https://hdl.handle.net/1822/44377 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Monteiro I. P., Shukla A., Marques A. P., Reis R. L., Hammond P. T. Spray-assisted layer-by-layer assembly on hyaluronic acid scaffolds for skin tissue engineering, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH A, Vol. 103, Issue 1, pp. 330-340, doi:10.1002/jbm.a.35178, 2015. 1552-4965 10.1002/jbm.a.35178 24659574 http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35178/full |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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