Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filipa
Data de Publicação: 2013
Outros Autores: Duarte, João, Simoes, Ana Rita, Cruz, Pedro F., Jones, John G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/109713
https://doi.org/10.1155/2013/638085
Resumo: The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions.
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spelling Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucoseAcetyl Coenzyme AAnimalsBlood GlucoseCarbon RadioisotopesCytosolFatty AcidsFructoseGlucoseLiverMiceThe (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions.Hindawi2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109713http://hdl.handle.net/10316/109713https://doi.org/10.1155/2013/638085eng2314-61332314-6141Carvalho, FilipaDuarte, JoãoSimoes, Ana RitaCruz, Pedro F.Jones, John G.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-23T11:42:11Zoai:estudogeral.uc.pt:10316/109713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:51.979357Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
title Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
spellingShingle Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
Carvalho, Filipa
Acetyl Coenzyme A
Animals
Blood Glucose
Carbon Radioisotopes
Cytosol
Fatty Acids
Fructose
Glucose
Liver
Mice
title_short Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
title_full Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
title_fullStr Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
title_full_unstemmed Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
title_sort Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
author Carvalho, Filipa
author_facet Carvalho, Filipa
Duarte, João
Simoes, Ana Rita
Cruz, Pedro F.
Jones, John G.
author_role author
author2 Duarte, João
Simoes, Ana Rita
Cruz, Pedro F.
Jones, John G.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Filipa
Duarte, João
Simoes, Ana Rita
Cruz, Pedro F.
Jones, John G.
dc.subject.por.fl_str_mv Acetyl Coenzyme A
Animals
Blood Glucose
Carbon Radioisotopes
Cytosol
Fatty Acids
Fructose
Glucose
Liver
Mice
topic Acetyl Coenzyme A
Animals
Blood Glucose
Carbon Radioisotopes
Cytosol
Fatty Acids
Fructose
Glucose
Liver
Mice
description The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/109713
http://hdl.handle.net/10316/109713
https://doi.org/10.1155/2013/638085
url http://hdl.handle.net/10316/109713
https://doi.org/10.1155/2013/638085
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2314-6133
2314-6141
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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