Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109713 https://doi.org/10.1155/2013/638085 |
Resumo: | The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucoseAcetyl Coenzyme AAnimalsBlood GlucoseCarbon RadioisotopesCytosolFatty AcidsFructoseGlucoseLiverMiceThe (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions.Hindawi2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109713http://hdl.handle.net/10316/109713https://doi.org/10.1155/2013/638085eng2314-61332314-6141Carvalho, FilipaDuarte, JoãoSimoes, Ana RitaCruz, Pedro F.Jones, John G.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-23T11:42:11Zoai:estudogeral.uc.pt:10316/109713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:51.979357Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
title |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
spellingShingle |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose Carvalho, Filipa Acetyl Coenzyme A Animals Blood Glucose Carbon Radioisotopes Cytosol Fatty Acids Fructose Glucose Liver Mice |
title_short |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
title_full |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
title_fullStr |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
title_full_unstemmed |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
title_sort |
Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose |
author |
Carvalho, Filipa |
author_facet |
Carvalho, Filipa Duarte, João Simoes, Ana Rita Cruz, Pedro F. Jones, John G. |
author_role |
author |
author2 |
Duarte, João Simoes, Ana Rita Cruz, Pedro F. Jones, John G. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Filipa Duarte, João Simoes, Ana Rita Cruz, Pedro F. Jones, John G. |
dc.subject.por.fl_str_mv |
Acetyl Coenzyme A Animals Blood Glucose Carbon Radioisotopes Cytosol Fatty Acids Fructose Glucose Liver Mice |
topic |
Acetyl Coenzyme A Animals Blood Glucose Carbon Radioisotopes Cytosol Fatty Acids Fructose Glucose Liver Mice |
description |
The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109713 http://hdl.handle.net/10316/109713 https://doi.org/10.1155/2013/638085 |
url |
http://hdl.handle.net/10316/109713 https://doi.org/10.1155/2013/638085 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2314-6133 2314-6141 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Hindawi |
publisher.none.fl_str_mv |
Hindawi |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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_version_ |
1799134140259368960 |