Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/49733 |
Resumo: | When growing microalgae for biorefinery processes, a high product yield is desired. For that reason, monitoring the concentration of the desired products during growth and products induction procedure is of great interest. 2D Fluorescence spectroscopy is a fingerprinting technique, used in situ and at real time, with a high potential for online monitoring of biological systems. In this work, Dunaliella salina pigment content was monitored using fluorescence data coupled with chemometric tools. Climatic parameters were also used as input variables due to their impact on the pigments profile in outdoor cultivations. Predictive models were developed for chlorophyll content (a, b, and total) with variance captured between 50 and 90%, and R2 varying between 0.6 and 0.9 for both training and validation data sets. Total carotenoids models captured 70 to 80% of variance, and R2 between 0.7 and 0.9, for training and validation. Models for specific carotenoids (zeaxanthin, α-carotene, all-trans-β-carotene, and 9-cis-β-carotene) captured variance between 60 and 90%, with validation and training R2 between 0.6 and 0.9. With this methodology, it was possible to calibrate a monitoring tool for pigments quantification, as a bulk and as individual compounds, proving that 2D fluorescence spectroscopy and climatic data combined with chemometric tools can be used to assess simultaneously and at real time different pigments in D. salina biomass production. |
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Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salinaWhen growing microalgae for biorefinery processes, a high product yield is desired. For that reason, monitoring the concentration of the desired products during growth and products induction procedure is of great interest. 2D Fluorescence spectroscopy is a fingerprinting technique, used in situ and at real time, with a high potential for online monitoring of biological systems. In this work, Dunaliella salina pigment content was monitored using fluorescence data coupled with chemometric tools. Climatic parameters were also used as input variables due to their impact on the pigments profile in outdoor cultivations. Predictive models were developed for chlorophyll content (a, b, and total) with variance captured between 50 and 90%, and R2 varying between 0.6 and 0.9 for both training and validation data sets. Total carotenoids models captured 70 to 80% of variance, and R2 between 0.7 and 0.9, for training and validation. Models for specific carotenoids (zeaxanthin, α-carotene, all-trans-β-carotene, and 9-cis-β-carotene) captured variance between 60 and 90%, with validation and training R2 between 0.6 and 0.9. With this methodology, it was possible to calibrate a monitoring tool for pigments quantification, as a bulk and as individual compounds, proving that 2D fluorescence spectroscopy and climatic data combined with chemometric tools can be used to assess simultaneously and at real time different pigments in D. salina biomass production.SpringerRepositório da Universidade de LisboaSá, MartaRamos, AlziraMonte, JoanaBrazinha, CarlaGalinha, Claudia F.Crespo, João G2021-09-30T14:09:15Z2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49733engSá, M., Ramos, A., Monte, J. et al. Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina. J Appl Phycol 32, 363–373 (2020). https://doi.org/10.1007/s10811-019-01999-z10.1007/s10811-019-01999-zinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:14Zoai:repositorio.ul.pt:10451/49733Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:05.443917Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
title |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
spellingShingle |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina Sá, Marta |
title_short |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
title_full |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
title_fullStr |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
title_full_unstemmed |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
title_sort |
Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina |
author |
Sá, Marta |
author_facet |
Sá, Marta Ramos, Alzira Monte, Joana Brazinha, Carla Galinha, Claudia F. Crespo, João G |
author_role |
author |
author2 |
Ramos, Alzira Monte, Joana Brazinha, Carla Galinha, Claudia F. Crespo, João G |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Sá, Marta Ramos, Alzira Monte, Joana Brazinha, Carla Galinha, Claudia F. Crespo, João G |
description |
When growing microalgae for biorefinery processes, a high product yield is desired. For that reason, monitoring the concentration of the desired products during growth and products induction procedure is of great interest. 2D Fluorescence spectroscopy is a fingerprinting technique, used in situ and at real time, with a high potential for online monitoring of biological systems. In this work, Dunaliella salina pigment content was monitored using fluorescence data coupled with chemometric tools. Climatic parameters were also used as input variables due to their impact on the pigments profile in outdoor cultivations. Predictive models were developed for chlorophyll content (a, b, and total) with variance captured between 50 and 90%, and R2 varying between 0.6 and 0.9 for both training and validation data sets. Total carotenoids models captured 70 to 80% of variance, and R2 between 0.7 and 0.9, for training and validation. Models for specific carotenoids (zeaxanthin, α-carotene, all-trans-β-carotene, and 9-cis-β-carotene) captured variance between 60 and 90%, with validation and training R2 between 0.6 and 0.9. With this methodology, it was possible to calibrate a monitoring tool for pigments quantification, as a bulk and as individual compounds, proving that 2D fluorescence spectroscopy and climatic data combined with chemometric tools can be used to assess simultaneously and at real time different pigments in D. salina biomass production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2020-02-01T00:00:00Z 2021-09-30T14:09:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/49733 |
url |
http://hdl.handle.net/10451/49733 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sá, M., Ramos, A., Monte, J. et al. Development of a monitoring tool based on fluorescence and climatic data for pigments profile estimation in Dunaliella salina. J Appl Phycol 32, 363–373 (2020). https://doi.org/10.1007/s10811-019-01999-z 10.1007/s10811-019-01999-z |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134558857199616 |