Nutritional requirements and strain heterogeneity in Ashbya gossypii

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Orquídea
Data de Publicação: 2012
Outros Autores: Domingues, Lucília, Penttilä, Merja, Wiebe, Marilyn G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22463
Resumo: Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.
id RCAP_1c33bfcfa4dd422fe2ddcee1238f3e0c
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/22463
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Nutritional requirements and strain heterogeneity in Ashbya gossypiiAshbya gossypiiNitrogen utilizationCarbon utilizationColony radial growth rateEthanolPHScience & TechnologyColony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.The financial support of Fundacao para a Ciencia e a Tecnologia (FCT), Portugal, is acknowledged, project AshByofactory PTDC/EBB-EBI/101985/2008 and grant SFRH/BD/30229/2006 to O. Ribeiro.WileyUniversidade do MinhoRibeiro, OrquídeaDomingues, LucíliaPenttilä, MerjaWiebe, Marilyn G.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22463eng0233-111X10.1002/jobm.20110038322143993info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:08Zoai:repositorium.sdum.uminho.pt:1822/22463Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:08.510577Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nutritional requirements and strain heterogeneity in Ashbya gossypii
title Nutritional requirements and strain heterogeneity in Ashbya gossypii
spellingShingle Nutritional requirements and strain heterogeneity in Ashbya gossypii
Ribeiro, Orquídea
Ashbya gossypii
Nitrogen utilization
Carbon utilization
Colony radial growth rate
Ethanol
PH
Science & Technology
title_short Nutritional requirements and strain heterogeneity in Ashbya gossypii
title_full Nutritional requirements and strain heterogeneity in Ashbya gossypii
title_fullStr Nutritional requirements and strain heterogeneity in Ashbya gossypii
title_full_unstemmed Nutritional requirements and strain heterogeneity in Ashbya gossypii
title_sort Nutritional requirements and strain heterogeneity in Ashbya gossypii
author Ribeiro, Orquídea
author_facet Ribeiro, Orquídea
Domingues, Lucília
Penttilä, Merja
Wiebe, Marilyn G.
author_role author
author2 Domingues, Lucília
Penttilä, Merja
Wiebe, Marilyn G.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ribeiro, Orquídea
Domingues, Lucília
Penttilä, Merja
Wiebe, Marilyn G.
dc.subject.por.fl_str_mv Ashbya gossypii
Nitrogen utilization
Carbon utilization
Colony radial growth rate
Ethanol
PH
Science & Technology
topic Ashbya gossypii
Nitrogen utilization
Carbon utilization
Colony radial growth rate
Ethanol
PH
Science & Technology
description Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at pH 4.5 was observed only on complex medium. D-Glucose, glycerol and starch were utilised as carbon sources. Ethanol was produced during growth on glycerol. Conversion of xylose into xylitol demonstrates that the xylose reductase is active. Phenotypic differences between related strains were greater than expected. We demonstrate that A. gossypii utilizes ammonium as sole nitrogen source at pH 6.5, facilitating further physiological studies using chemically defined media in the future.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22463
url http://hdl.handle.net/1822/22463
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0233-111X
10.1002/jobm.201100383
22143993
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132832570802176

Registros relacionados