Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate
Main Author: | |
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Publication Date: | 2009 |
Other Authors: | |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | https://hdl.handle.net/1822/20319 |
Summary: | This paper reports the effect of a-amylase encapsulation on the degradation rate of a starch-based biomaterial. The encapsulation method consisted in mixing a thermostable a-amylase with a blend of corn starch and polycaprolactone (SPCL), which were processed by compression moulding to produce circular disks. The presence of water was avoided to keep the water activity low and consequently to minimize the enzyme activity during the encapsulation process. No degradation of the starch matrix occurred during processing and storage (the encapsulated enzyme remained inactive due to the absence of water), since no significant amount of reducing sugars was detected in solution. After the encapsulation process, the released enzyme activity from the SPCL disks after 28 days was found to be 40% comparatively to the free enzyme (unprocessed). Degradation studies on SPCL disks, with a-amylase encapsulated or free in solution, showed no significant differences on the degradation behaviour between both conditions. This indicates that a-amylase enzyme was successfully encapsulated with almost full retention of its enzymatic activity and the encapsulation of a-amylase clearly accelerates the degradation rate of the SPCL disks, when compared with the enzyme-free disks. The results obtained in this work show that degradation kinetics of the starch polymer can be controlled by the amount of encapsulated a-amylase into the matrix. |
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Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rateStarch-based biomaterialsControlled degradation rateThermostable a-amylaseEncapsulationEnzyme activityScience & TechnologyThis paper reports the effect of a-amylase encapsulation on the degradation rate of a starch-based biomaterial. The encapsulation method consisted in mixing a thermostable a-amylase with a blend of corn starch and polycaprolactone (SPCL), which were processed by compression moulding to produce circular disks. The presence of water was avoided to keep the water activity low and consequently to minimize the enzyme activity during the encapsulation process. No degradation of the starch matrix occurred during processing and storage (the encapsulated enzyme remained inactive due to the absence of water), since no significant amount of reducing sugars was detected in solution. After the encapsulation process, the released enzyme activity from the SPCL disks after 28 days was found to be 40% comparatively to the free enzyme (unprocessed). Degradation studies on SPCL disks, with a-amylase encapsulated or free in solution, showed no significant differences on the degradation behaviour between both conditions. This indicates that a-amylase enzyme was successfully encapsulated with almost full retention of its enzymatic activity and the encapsulation of a-amylase clearly accelerates the degradation rate of the SPCL disks, when compared with the enzyme-free disks. The results obtained in this work show that degradation kinetics of the starch polymer can be controlled by the amount of encapsulated a-amylase into the matrix.This work was partially supported by Portuguese Foundation for Science and Technology (FCT) through funds from the POCTI and/or FEDER Programmes. This work was carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283).ElsevierUniversidade do MinhoAzevedo, Helena S.Reis, R. L.20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/20319eng1742-706110.1016/j.actbio.2009.04.03919427418http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:50Zoai:repositorium.sdum.uminho.pt:1822/20319Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:11.740194Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
title |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
spellingShingle |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate Azevedo, Helena S. Starch-based biomaterials Controlled degradation rate Thermostable a-amylase Encapsulation Enzyme activity Science & Technology |
title_short |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
title_full |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
title_fullStr |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
title_full_unstemmed |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
title_sort |
Encapsulation of alpha-amylase into starch-based biomaterials: an enzymatic approach to tailor their degradation rate |
author |
Azevedo, Helena S. |
author_facet |
Azevedo, Helena S. Reis, R. L. |
author_role |
author |
author2 |
Reis, R. L. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Azevedo, Helena S. Reis, R. L. |
dc.subject.por.fl_str_mv |
Starch-based biomaterials Controlled degradation rate Thermostable a-amylase Encapsulation Enzyme activity Science & Technology |
topic |
Starch-based biomaterials Controlled degradation rate Thermostable a-amylase Encapsulation Enzyme activity Science & Technology |
description |
This paper reports the effect of a-amylase encapsulation on the degradation rate of a starch-based biomaterial. The encapsulation method consisted in mixing a thermostable a-amylase with a blend of corn starch and polycaprolactone (SPCL), which were processed by compression moulding to produce circular disks. The presence of water was avoided to keep the water activity low and consequently to minimize the enzyme activity during the encapsulation process. No degradation of the starch matrix occurred during processing and storage (the encapsulated enzyme remained inactive due to the absence of water), since no significant amount of reducing sugars was detected in solution. After the encapsulation process, the released enzyme activity from the SPCL disks after 28 days was found to be 40% comparatively to the free enzyme (unprocessed). Degradation studies on SPCL disks, with a-amylase encapsulated or free in solution, showed no significant differences on the degradation behaviour between both conditions. This indicates that a-amylase enzyme was successfully encapsulated with almost full retention of its enzymatic activity and the encapsulation of a-amylase clearly accelerates the degradation rate of the SPCL disks, when compared with the enzyme-free disks. The results obtained in this work show that degradation kinetics of the starch polymer can be controlled by the amount of encapsulated a-amylase into the matrix. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/20319 |
url |
https://hdl.handle.net/1822/20319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1742-7061 10.1016/j.actbio.2009.04.039 19427418 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132489976905728 |