Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/1485 |
Resumo: | Two enzymatic extracts obtained from xylan-grown Aspergillus terreus CCMI 498 and cellulose-grown Trichoderma viride CCMI 84 were characterised for different glycanase activities. Both strains produce extracellular endoxylanase and endoglucanase enzymes. The enzymes optimal activity was found in the temperature range of 45-60 ºC. Endoglucanase systems show identical activity profiles towards temperature, regardless of the strain and inducing substrate. Conversely, the endoxylanases produced by both strains showed maximal activity at different pH values (from 4.5 to 5.5), being the more acidic xylanase produced by T. viride grown on cellulose. The endoglucanase activities have an optimum pH at 4.5-5.0. The endoxylanase and endoglucanase activities exhibited high stability at 50 ºC and pH 5.0. Mannanase, β-xylosidase, and amylase activities were also found, being the first two activities only present for T. viride extract. These two enzymatic extracts were used for mixed office wastepaper (MOW) deinking. When the enzymatic extract from T. viride was used, a further increase of 24% in ink removal was obtained by comparison with the control. Both enzymes contributed to the improvement of the paper strength properties and the obtained results clearly indicate that the effective use of enzymes for deinking can also contribute to the pulp and paper properties improvement. |
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Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaperHemicellulaseEndoglucanaseAmylaseEnzymatic deinkingAspergillus terreusTrichoderma virideScience & TechnologyTwo enzymatic extracts obtained from xylan-grown Aspergillus terreus CCMI 498 and cellulose-grown Trichoderma viride CCMI 84 were characterised for different glycanase activities. Both strains produce extracellular endoxylanase and endoglucanase enzymes. The enzymes optimal activity was found in the temperature range of 45-60 ºC. Endoglucanase systems show identical activity profiles towards temperature, regardless of the strain and inducing substrate. Conversely, the endoxylanases produced by both strains showed maximal activity at different pH values (from 4.5 to 5.5), being the more acidic xylanase produced by T. viride grown on cellulose. The endoglucanase activities have an optimum pH at 4.5-5.0. The endoxylanase and endoglucanase activities exhibited high stability at 50 ºC and pH 5.0. Mannanase, β-xylosidase, and amylase activities were also found, being the first two activities only present for T. viride extract. These two enzymatic extracts were used for mixed office wastepaper (MOW) deinking. When the enzymatic extract from T. viride was used, a further increase of 24% in ink removal was obtained by comparison with the control. Both enzymes contributed to the improvement of the paper strength properties and the obtained results clearly indicate that the effective use of enzymes for deinking can also contribute to the pulp and paper properties improvement.PRAXIS/BIO/1133/95. BIC/3087/96, BD/3253/96.Elsevier ScienceUniversidade do MinhoMarques, S.Pala, H.Alves, L.Amaral-Collaço, M. T.Gama, F. M.Gírio, F. M.20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/1485engMarques, S., Pala, H., Alves, L., Amaral-Collaço, M. T., Gama, F. M., & Gı́rio, F. M. (2003, February). Characterisation and application of glycanases secreted by Aspergillus terreus CCMI 498 and Trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper. Journal of Biotechnology. Elsevier BV. http://doi.org/10.1016/s0168-1656(02)00247-x0168-165610.1016/S0168-1656(02)00247-X12443852info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:28Zoai:repositorium.sdum.uminho.pt:1822/1485Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:17:12.255332Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
title |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
spellingShingle |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper Marques, S. Hemicellulase Endoglucanase Amylase Enzymatic deinking Aspergillus terreus Trichoderma viride Science & Technology |
title_short |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
title_full |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
title_fullStr |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
title_full_unstemmed |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
title_sort |
Characterisation and application of glycanases secreted by aspergillus terreus CCMI 498 and trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper |
author |
Marques, S. |
author_facet |
Marques, S. Pala, H. Alves, L. Amaral-Collaço, M. T. Gama, F. M. Gírio, F. M. |
author_role |
author |
author2 |
Pala, H. Alves, L. Amaral-Collaço, M. T. Gama, F. M. Gírio, F. M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Marques, S. Pala, H. Alves, L. Amaral-Collaço, M. T. Gama, F. M. Gírio, F. M. |
dc.subject.por.fl_str_mv |
Hemicellulase Endoglucanase Amylase Enzymatic deinking Aspergillus terreus Trichoderma viride Science & Technology |
topic |
Hemicellulase Endoglucanase Amylase Enzymatic deinking Aspergillus terreus Trichoderma viride Science & Technology |
description |
Two enzymatic extracts obtained from xylan-grown Aspergillus terreus CCMI 498 and cellulose-grown Trichoderma viride CCMI 84 were characterised for different glycanase activities. Both strains produce extracellular endoxylanase and endoglucanase enzymes. The enzymes optimal activity was found in the temperature range of 45-60 ºC. Endoglucanase systems show identical activity profiles towards temperature, regardless of the strain and inducing substrate. Conversely, the endoxylanases produced by both strains showed maximal activity at different pH values (from 4.5 to 5.5), being the more acidic xylanase produced by T. viride grown on cellulose. The endoglucanase activities have an optimum pH at 4.5-5.0. The endoxylanase and endoglucanase activities exhibited high stability at 50 ºC and pH 5.0. Mannanase, β-xylosidase, and amylase activities were also found, being the first two activities only present for T. viride extract. These two enzymatic extracts were used for mixed office wastepaper (MOW) deinking. When the enzymatic extract from T. viride was used, a further increase of 24% in ink removal was obtained by comparison with the control. Both enzymes contributed to the improvement of the paper strength properties and the obtained results clearly indicate that the effective use of enzymes for deinking can also contribute to the pulp and paper properties improvement. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2003-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/1485 |
url |
https://hdl.handle.net/1822/1485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marques, S., Pala, H., Alves, L., Amaral-Collaço, M. T., Gama, F. M., & Gı́rio, F. M. (2003, February). Characterisation and application of glycanases secreted by Aspergillus terreus CCMI 498 and Trichoderma viride CCMI 84 for enzymatic deinking of mixed office wastepaper. Journal of Biotechnology. Elsevier BV. http://doi.org/10.1016/s0168-1656(02)00247-x 0168-1656 10.1016/S0168-1656(02)00247-X 12443852 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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