Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4696 |
Resumo: | Functionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis. |
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Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung deliveryLung cancerDry powder formulationPulmonary deliveryGold nanoparticlesNano-in-microSupercritical CO2Spray dryingFunctionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis.ElsevieruBibliorumSilva, A. SofiaSousa, Ana M. L.Cabral, RenatoSilva, MartaSequeira, Clarinda CostaMiguel, SóniaBonifácio, VascoCasimiro, TeresaCorreia, I.J.Ricardo, Ana Aguiar2018-03-22T09:04:40Z2017-01-192017-01-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4696engSilva, A. S., Sousa, A. M., Cabral, R. P., Correia, I. J., e Aguiar-Ricardo, A. (2017) “Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery.” International Journal of Pharmaceutics, Vol.519 (1-2), pp.240-24910.1016/j.ijpharm.2017.01.032metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:31:45Zoai:ubibliorum.ubi.pt:10400.6/4696Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:44.914313Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
title |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
spellingShingle |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery Silva, A. Sofia Lung cancer Dry powder formulation Pulmonary delivery Gold nanoparticles Nano-in-micro Supercritical CO2 Spray drying |
title_short |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
title_full |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
title_fullStr |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
title_full_unstemmed |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
title_sort |
Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery |
author |
Silva, A. Sofia |
author_facet |
Silva, A. Sofia Sousa, Ana M. L. Cabral, Renato Silva, Marta Sequeira, Clarinda Costa Miguel, Sónia Bonifácio, Vasco Casimiro, Teresa Correia, I.J. Ricardo, Ana Aguiar |
author_role |
author |
author2 |
Sousa, Ana M. L. Cabral, Renato Silva, Marta Sequeira, Clarinda Costa Miguel, Sónia Bonifácio, Vasco Casimiro, Teresa Correia, I.J. Ricardo, Ana Aguiar |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Silva, A. Sofia Sousa, Ana M. L. Cabral, Renato Silva, Marta Sequeira, Clarinda Costa Miguel, Sónia Bonifácio, Vasco Casimiro, Teresa Correia, I.J. Ricardo, Ana Aguiar |
dc.subject.por.fl_str_mv |
Lung cancer Dry powder formulation Pulmonary delivery Gold nanoparticles Nano-in-micro Supercritical CO2 Spray drying |
topic |
Lung cancer Dry powder formulation Pulmonary delivery Gold nanoparticles Nano-in-micro Supercritical CO2 Spray drying |
description |
Functionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-19 2017-01-19T00:00:00Z 2018-03-22T09:04:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4696 |
url |
http://hdl.handle.net/10400.6/4696 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, A. S., Sousa, A. M., Cabral, R. P., Correia, I. J., e Aguiar-Ricardo, A. (2017) “Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery.” International Journal of Pharmaceutics, Vol.519 (1-2), pp.240-249 10.1016/j.ijpharm.2017.01.032 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136354536259584 |