Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery

Detalhes bibliográficos
Autor(a) principal: Silva, A. Sofia
Data de Publicação: 2017
Outros Autores: Sousa, Ana M. L., Cabral, Renato, Silva, Marta, Sequeira, Clarinda Costa, Miguel, Sónia, Bonifácio, Vasco, Casimiro, Teresa, Correia, I.J., Ricardo, Ana Aguiar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4696
Resumo: Functionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis.
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spelling Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung deliveryLung cancerDry powder formulationPulmonary deliveryGold nanoparticlesNano-in-microSupercritical CO2Spray dryingFunctionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis.ElsevieruBibliorumSilva, A. SofiaSousa, Ana M. L.Cabral, RenatoSilva, MartaSequeira, Clarinda CostaMiguel, SóniaBonifácio, VascoCasimiro, TeresaCorreia, I.J.Ricardo, Ana Aguiar2018-03-22T09:04:40Z2017-01-192017-01-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4696engSilva, A. S., Sousa, A. M., Cabral, R. P., Correia, I. J., e Aguiar-Ricardo, A. (2017) “Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery.” International Journal of Pharmaceutics, Vol.519 (1-2), pp.240-24910.1016/j.ijpharm.2017.01.032metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-31T02:31:45Zoai:ubibliorum.ubi.pt:10400.6/4696Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:44.914313Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
title Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
spellingShingle Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
Silva, A. Sofia
Lung cancer
Dry powder formulation
Pulmonary delivery
Gold nanoparticles
Nano-in-micro
Supercritical CO2
Spray drying
title_short Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
title_full Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
title_fullStr Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
title_full_unstemmed Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
title_sort Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery
author Silva, A. Sofia
author_facet Silva, A. Sofia
Sousa, Ana M. L.
Cabral, Renato
Silva, Marta
Sequeira, Clarinda Costa
Miguel, Sónia
Bonifácio, Vasco
Casimiro, Teresa
Correia, I.J.
Ricardo, Ana Aguiar
author_role author
author2 Sousa, Ana M. L.
Cabral, Renato
Silva, Marta
Sequeira, Clarinda Costa
Miguel, Sónia
Bonifácio, Vasco
Casimiro, Teresa
Correia, I.J.
Ricardo, Ana Aguiar
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Silva, A. Sofia
Sousa, Ana M. L.
Cabral, Renato
Silva, Marta
Sequeira, Clarinda Costa
Miguel, Sónia
Bonifácio, Vasco
Casimiro, Teresa
Correia, I.J.
Ricardo, Ana Aguiar
dc.subject.por.fl_str_mv Lung cancer
Dry powder formulation
Pulmonary delivery
Gold nanoparticles
Nano-in-micro
Supercritical CO2
Spray drying
topic Lung cancer
Dry powder formulation
Pulmonary delivery
Gold nanoparticles
Nano-in-micro
Supercritical CO2
Spray drying
description Functionalized gold nanoparticles (AuNPs) have been widely investigated as promising multifunctional nanosystems for the theragnosis of lung cancer, the most common and prominent cause of cancer death worldwide. Nevertheless, nanoparticles are not in appropriate sizes for an accurate deep lung delivery and the lack of locally and effective delivery of therapeutic biomolecules to the deep lungs is, in fact, the major cause of low therapeutic outcome. Herein we incorporate, for the first time, AuNPs into respirable microparticles. AuNPs were functionalized with biocompatible oligo(2-oxazoline)-based optically stable fluorescent coatings, and conjugated with a laminin peptide (YIGSR) for targeted lung cancer delivery. These POxylated AuNPs were then incorporated into a chitosan matrix by a clean process, supercritical CO2-assisted spray drying (SASD), yielding nano-in-micro clean ultrafine dry powder formulations. The engineered formulations present the adequate morphology and flowability to reach the deep lung, with aerodynamic sizes ranging 3.2–3.8 μm, and excellent fine particle fraction (FPF) (FPF of 47% for CHT-bearing targeted AuNPs). The optimal biodegradation and release profiles enabled a sustained and controlled release of the embedded nanoparticles, with enhanced cellular uptake, opening new prospects for future lung theragnosis.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-19
2017-01-19T00:00:00Z
2018-03-22T09:04:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4696
url http://hdl.handle.net/10400.6/4696
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, A. S., Sousa, A. M., Cabral, R. P., Correia, I. J., e Aguiar-Ricardo, A. (2017) “Aerosolizable gold nano-in-micro dry powder formulations for theragnosis and lung delivery.” International Journal of Pharmaceutics, Vol.519 (1-2), pp.240-249
10.1016/j.ijpharm.2017.01.032
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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