The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Ferreira, Mário J.
Data de Publicação: 2017
Outros Autores: Mendes, Aristides L., Sá-Nogueira, Isabel de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1371/journal.pone.0189483
Resumo: This work was supported by Fundagao para a Ciencia e a Tecnologia (FCT/MCTES http://www.fct.pt/index.phtml.pt) [PEst-OE/BIA/U10457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.]. Lia Godinho constructed pLG31, Grac E a Susete constructed pGS1 and strain IQB622, Liliana Brito constructed pLB1 and strain IQB623, and Joana Lima constructed pJL1. Lu o A s Jaime Mota, Adriano O. Henriques, and Mariana G. Pinho provided the antibodies used for immunoblotting. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT/MCTES) [PEst-OE/BIA/UI0457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.].
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spelling The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilisBINDING CASSETTE TRANSPORTERABC TRANSPORTERSFUNCTIONAL-ANALYSISDEGRADATIONGENEMALTOSEBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)This work was supported by Fundagao para a Ciencia e a Tecnologia (FCT/MCTES http://www.fct.pt/index.phtml.pt) [PEst-OE/BIA/U10457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.]. Lia Godinho constructed pLG31, Grac E a Susete constructed pGS1 and strain IQB622, Liliana Brito constructed pLB1 and strain IQB623, and Joana Lima constructed pJL1. Lu o A s Jaime Mota, Adriano O. Henriques, and Mariana G. Pinho provided the antibodies used for immunoblotting. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT/MCTES) [PEst-OE/BIA/UI0457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.].Carbohydrates from plant cell walls are often found as heteropolysaccharides intertwined with each other. For competitive advantage against other microorganisms, and ability to fully exploit available carbon and energy sources, Bacillus subtilis possesses a high number of proteins dedicated to the uptake of mono- and oligosaccharides. Here, we characterize transporter complexes, belonging to the ATP-binding cassette (ABC) superfamily, involved in the uptake of oligosaccharides commonly found in pectin. The uptake of these carbohydrates is shown to be MsmX-dependent, assigning a key role in pectin mobilization for MsmX, a multipurpose ATPase serving several distinct ABC-type I sugar importers. Muta-genesis analysis of the transmembrane domains of the AraNPQ MsmX-dependent importer revealed putative residues for MsmX interaction. Interestingly however, although MsmX is shown to be essential for energizing various ABC transporters we found that a second B. subtilis ATPase, YurJ, is able to complement its function when placed in trans at a different locus of the chromosome.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNFerreira, Mário J.Mendes, Aristides L.Sá-Nogueira, Isabel de2018-05-11T22:11:28Z2017-12-012017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0189483eng1932-6203PURE: 3818461http://www.scopus.com/inward/record.url?scp=85038638853&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0189483info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:20Zoai:run.unl.pt:10362/36721Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:39.352208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
title The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
spellingShingle The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
Ferreira, Mário J.
BINDING CASSETTE TRANSPORTER
ABC TRANSPORTERS
FUNCTIONAL-ANALYSIS
DEGRADATION
GENE
MALTOSE
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
title_short The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
title_full The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
title_fullStr The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
title_full_unstemmed The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
title_sort The MsmX ATPase plays a crucial role in pectin mobilization by Bacillus subtilis
author Ferreira, Mário J.
author_facet Ferreira, Mário J.
Mendes, Aristides L.
Sá-Nogueira, Isabel de
author_role author
author2 Mendes, Aristides L.
Sá-Nogueira, Isabel de
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Ferreira, Mário J.
Mendes, Aristides L.
Sá-Nogueira, Isabel de
dc.subject.por.fl_str_mv BINDING CASSETTE TRANSPORTER
ABC TRANSPORTERS
FUNCTIONAL-ANALYSIS
DEGRADATION
GENE
MALTOSE
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
topic BINDING CASSETTE TRANSPORTER
ABC TRANSPORTERS
FUNCTIONAL-ANALYSIS
DEGRADATION
GENE
MALTOSE
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
description This work was supported by Fundagao para a Ciencia e a Tecnologia (FCT/MCTES http://www.fct.pt/index.phtml.pt) [PEst-OE/BIA/U10457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.]. Lia Godinho constructed pLG31, Grac E a Susete constructed pGS1 and strain IQB622, Liliana Brito constructed pLB1 and strain IQB623, and Joana Lima constructed pJL1. Lu o A s Jaime Mota, Adriano O. Henriques, and Mariana G. Pinho provided the antibodies used for immunoblotting. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT/MCTES) [PEst-OE/BIA/UI0457/2011 to Centro de Recursos Microbiologicos (CREM); UID/Multi/04378/2013 to Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); fellowship SFRH/BD/73039/2010 to M.J.F.].
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
2017-12-01T00:00:00Z
2018-05-11T22:11:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1371/journal.pone.0189483
url https://doi.org/10.1371/journal.pone.0189483
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 3818461
http://www.scopus.com/inward/record.url?scp=85038638853&partnerID=8YFLogxK
https://doi.org/10.1371/journal.pone.0189483
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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