Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/32992 |
Resumo: | As the leading cause of death worldwide, myocardial infarction (MI) carries considerable socioeconomic costs. The damage and clinical complications caused by MI remain unaddressed by current therapeutic strategies. With the advent of tissue engineering and regenerative medicine, novel strategies to promote regeneration of heart muscle are possible. This dissertation focuses on the application of microcapsule-based systems to promote the recovery of damaged tissue. The biofabrication of core-shell microcapsules for the encapsulation of cells and bioactive molecules has been reviewed, along with the contributions of these platforms to the field of regenerative medicine. In this work, coaxial electrospray technology has been used to encapsulate methacryloyl platelet lysate (PLMA) within alginate microcapsules, producing sacrificial templates for the preparation of porous microcarriers. By exposing the capsules to UV light, it was possible to induce the photopolymerization of PLMA. The alginate shell was removed and the resulting particles were subjected to a freeze-drying procedure, producing PLMA microsponges. By adjusting production parameters, the proposed system was able to produce microcapsules with core diameter ranging from 300 to 600 μm, as well as microcapsules with shell thickness below 100 μm. The microsponges prepared with this system were shown to allow cell attachment and promote the assembly of cardiac microtissues, indicating potential applications in tissue engineering. |
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Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarctionMyocardial infarctionMicrospongesLiquid core capsulesPlatelet lysateCoreshellAs the leading cause of death worldwide, myocardial infarction (MI) carries considerable socioeconomic costs. The damage and clinical complications caused by MI remain unaddressed by current therapeutic strategies. With the advent of tissue engineering and regenerative medicine, novel strategies to promote regeneration of heart muscle are possible. This dissertation focuses on the application of microcapsule-based systems to promote the recovery of damaged tissue. The biofabrication of core-shell microcapsules for the encapsulation of cells and bioactive molecules has been reviewed, along with the contributions of these platforms to the field of regenerative medicine. In this work, coaxial electrospray technology has been used to encapsulate methacryloyl platelet lysate (PLMA) within alginate microcapsules, producing sacrificial templates for the preparation of porous microcarriers. By exposing the capsules to UV light, it was possible to induce the photopolymerization of PLMA. The alginate shell was removed and the resulting particles were subjected to a freeze-drying procedure, producing PLMA microsponges. By adjusting production parameters, the proposed system was able to produce microcapsules with core diameter ranging from 300 to 600 μm, as well as microcapsules with shell thickness below 100 μm. The microsponges prepared with this system were shown to allow cell attachment and promote the assembly of cardiac microtissues, indicating potential applications in tissue engineering.Como principal causa de morte em todo o mundo, o enfarte do miocárdio (EM) gera custos socioeconómicos consideráveis. Os danos e complicações clínicas provocadas por EM não são adequadamente abordados pelas estratégias terapêuticas atuais. Com o advento da engenharia de tecidos e da medicina regenerativa, novas estratégias para promover a regeneração do músculo cardíaco são possíveis. Esta dissertação foca-se na aplicação de sistemas baseados em microcápsulas para promover a recuperação de tecidos danificados. A biofabricação de microcápsulas core-shell para o encapsulamento de células e moléculas bioativas foi revista, juntamente com as contribuições destas plataformas no campo da medicina regenerativa. Neste trabalho, a tecnologia de eletrospray coaxial foi usada para encapsular lisado de plaquetas metacrilado (PLMA) em microcápsulas de alginato, produzindo moldes sacrificiais para a preparação de microcarriers porosos. Ao expor as cápsulas à luz ultravioleta, foi possível induzir a fotopolimerização do PLMA. A shell de alginato foi removida e as partículas resultantes foram submetidas a um procedimento de liofilização, produzindo microesponjas de PLMA. Ajustando os parâmetros de produção, o sistema proposto foi capaz de produzir microcápsulas com cores de diâmetro compreendido no intervalo de 300 a 600 μm, bem como microcápsulas com shells possuindo espessuras abaixo de 100 μm. As microesponjas preparadas a partir deste sistema permitiram a adesão de células e promoveram a agregação em microtecidos cardíacos, indicando possíveis aplicações na engenharia de tecidos.2023-12-15T00:00:00Z2021-12-06T00:00:00Z2021-12-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/32992engLadeira, Bruno Miguel Fernandesinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:03:29Zoai:ria.ua.pt:10773/32992Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:04:31.341202Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
title |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
spellingShingle |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction Ladeira, Bruno Miguel Fernandes Myocardial infarction Microsponges Liquid core capsules Platelet lysate Coreshell |
title_short |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
title_full |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
title_fullStr |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
title_full_unstemmed |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
title_sort |
Development of platelet lysate based scaffolds from microcapsules for regeneration post-myocardial infarction |
author |
Ladeira, Bruno Miguel Fernandes |
author_facet |
Ladeira, Bruno Miguel Fernandes |
author_role |
author |
dc.contributor.author.fl_str_mv |
Ladeira, Bruno Miguel Fernandes |
dc.subject.por.fl_str_mv |
Myocardial infarction Microsponges Liquid core capsules Platelet lysate Coreshell |
topic |
Myocardial infarction Microsponges Liquid core capsules Platelet lysate Coreshell |
description |
As the leading cause of death worldwide, myocardial infarction (MI) carries considerable socioeconomic costs. The damage and clinical complications caused by MI remain unaddressed by current therapeutic strategies. With the advent of tissue engineering and regenerative medicine, novel strategies to promote regeneration of heart muscle are possible. This dissertation focuses on the application of microcapsule-based systems to promote the recovery of damaged tissue. The biofabrication of core-shell microcapsules for the encapsulation of cells and bioactive molecules has been reviewed, along with the contributions of these platforms to the field of regenerative medicine. In this work, coaxial electrospray technology has been used to encapsulate methacryloyl platelet lysate (PLMA) within alginate microcapsules, producing sacrificial templates for the preparation of porous microcarriers. By exposing the capsules to UV light, it was possible to induce the photopolymerization of PLMA. The alginate shell was removed and the resulting particles were subjected to a freeze-drying procedure, producing PLMA microsponges. By adjusting production parameters, the proposed system was able to produce microcapsules with core diameter ranging from 300 to 600 μm, as well as microcapsules with shell thickness below 100 μm. The microsponges prepared with this system were shown to allow cell attachment and promote the assembly of cardiac microtissues, indicating potential applications in tissue engineering. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-06T00:00:00Z 2021-12-06 2023-12-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/32992 |
url |
http://hdl.handle.net/10773/32992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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