Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves

Detalhes bibliográficos
Autor(a) principal: Valencia-Salgado, Carlos
Data de Publicação: 2022
Outros Autores: Jacobo-Arreola, Selene, Said-Fernandez, Salvado, Soto-Dominguez, Adolfo, Camacho-Morales, Alberto, Martinez-Rodriguez, Herminia, Rodriguez-Rocha, Humberto, Delgado-Gonzalez, Paulina, Quiroz-Reyes, Adriana, Islas, Jose, Garza-Treviño, Elsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.48797/sl.2022.3
Resumo: We evaluated cell markers of oligodendrocyte-like cells (OLC) and neuron-like cells (NLC) differentiation from human adipose tissue-derived mesenchymal stem cells (hADSCs) cocultured on decellularized sciatic nerves. OLC and NLC were incubated at a 10:1 ratio with decellularized rat nerves. We estimated the percentage of myelin basic protein (MBP)- and neurofilament protein (NFP)-positive cells. OLC and NLC cocultures showed an increase in positive cells for MBP and proteolipid protein (PLP) markers. Oligodendrocytes and neurons cocultured with sciatic nerve scaffolds promoted myelination by OLC within 3 days, which remained stable for at least 21 days. We propose a reproducible experimental strategy to promote sciatic nerve myelination by coculturing hADSCs-derived OLC and NLC in nerve scaffolds.
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spelling Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nervesscaffoldsadipose tissue-derived mesenchymal stem cellsoligodendrocyte-like cellssciatic nerveWe evaluated cell markers of oligodendrocyte-like cells (OLC) and neuron-like cells (NLC) differentiation from human adipose tissue-derived mesenchymal stem cells (hADSCs) cocultured on decellularized sciatic nerves. OLC and NLC were incubated at a 10:1 ratio with decellularized rat nerves. We estimated the percentage of myelin basic protein (MBP)- and neurofilament protein (NFP)-positive cells. OLC and NLC cocultures showed an increase in positive cells for MBP and proteolipid protein (PLP) markers. Oligodendrocytes and neurons cocultured with sciatic nerve scaffolds promoted myelination by OLC within 3 days, which remained stable for at least 21 days. We propose a reproducible experimental strategy to promote sciatic nerve myelination by coculturing hADSCs-derived OLC and NLC in nerve scaffolds.IUCS-CESPU Publishing2022-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2022.3https://doi.org/10.48797/sl.2022.3Scientific Letters; Vol. 1 No. 1 (2022); 22795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/3https://publicacoes.cespu.pt/index.php/sl/article/view/3/3Copyright (c) 2022 Carlos I. Valencia-Salgado, Selene Jacobo-Arreola, Salvado L. Said-Fernandez, Adolfo Soto-Dominguez, Alberto Camacho-Morales, Herminia G. Martinez-Rodriguez, Humberto Rodriguez-Rocha, Paulina Delgado-Gonzalez, Adriana G. Quiroz-Reyes, Jose F. Islas, Elsa N. Garza-Treviñoinfo:eu-repo/semantics/openAccessValencia-Salgado, CarlosJacobo-Arreola, SeleneSaid-Fernandez, SalvadoSoto-Dominguez, AdolfoCamacho-Morales, AlbertoMartinez-Rodriguez, HerminiaRodriguez-Rocha, HumbertoDelgado-Gonzalez, PaulinaQuiroz-Reyes, AdrianaIslas, JoseGarza-Treviño, Elsa2023-04-22T08:45:12Zoai:publicacoes.cespu.pt:article/3Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:46:16.502241Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
title Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
spellingShingle Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
Valencia-Salgado, Carlos
scaffolds
adipose tissue-derived mesenchymal stem cells
oligodendrocyte-like cells
sciatic nerve
title_short Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
title_full Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
title_fullStr Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
title_full_unstemmed Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
title_sort Promoting differentiation of human adipose mesenchymal stem cells into oligodendrocyte-like cells and neuron-like cells through coculture on decellularized sciatic nerves
author Valencia-Salgado, Carlos
author_facet Valencia-Salgado, Carlos
Jacobo-Arreola, Selene
Said-Fernandez, Salvado
Soto-Dominguez, Adolfo
Camacho-Morales, Alberto
Martinez-Rodriguez, Herminia
Rodriguez-Rocha, Humberto
Delgado-Gonzalez, Paulina
Quiroz-Reyes, Adriana
Islas, Jose
Garza-Treviño, Elsa
author_role author
author2 Jacobo-Arreola, Selene
Said-Fernandez, Salvado
Soto-Dominguez, Adolfo
Camacho-Morales, Alberto
Martinez-Rodriguez, Herminia
Rodriguez-Rocha, Humberto
Delgado-Gonzalez, Paulina
Quiroz-Reyes, Adriana
Islas, Jose
Garza-Treviño, Elsa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valencia-Salgado, Carlos
Jacobo-Arreola, Selene
Said-Fernandez, Salvado
Soto-Dominguez, Adolfo
Camacho-Morales, Alberto
Martinez-Rodriguez, Herminia
Rodriguez-Rocha, Humberto
Delgado-Gonzalez, Paulina
Quiroz-Reyes, Adriana
Islas, Jose
Garza-Treviño, Elsa
dc.subject.por.fl_str_mv scaffolds
adipose tissue-derived mesenchymal stem cells
oligodendrocyte-like cells
sciatic nerve
topic scaffolds
adipose tissue-derived mesenchymal stem cells
oligodendrocyte-like cells
sciatic nerve
description We evaluated cell markers of oligodendrocyte-like cells (OLC) and neuron-like cells (NLC) differentiation from human adipose tissue-derived mesenchymal stem cells (hADSCs) cocultured on decellularized sciatic nerves. OLC and NLC were incubated at a 10:1 ratio with decellularized rat nerves. We estimated the percentage of myelin basic protein (MBP)- and neurofilament protein (NFP)-positive cells. OLC and NLC cocultures showed an increase in positive cells for MBP and proteolipid protein (PLP) markers. Oligodendrocytes and neurons cocultured with sciatic nerve scaffolds promoted myelination by OLC within 3 days, which remained stable for at least 21 days. We propose a reproducible experimental strategy to promote sciatic nerve myelination by coculturing hADSCs-derived OLC and NLC in nerve scaffolds.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.48797/sl.2022.3
https://doi.org/10.48797/sl.2022.3
url https://doi.org/10.48797/sl.2022.3
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://publicacoes.cespu.pt/index.php/sl/article/view/3
https://publicacoes.cespu.pt/index.php/sl/article/view/3/3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IUCS-CESPU Publishing
publisher.none.fl_str_mv IUCS-CESPU Publishing
dc.source.none.fl_str_mv Scientific Letters; Vol. 1 No. 1 (2022); 2
2795-5117
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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