Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/24287 |
Resumo: | The problematic increase in multidrug-resistant bacteria translates into the urgent need to discover novel and effective antimicrobial substances. Herein, mushrooms could be a promising alternative of natural source of new antimicrobials. The present work aimed to compare the phytochemical composition and antimicrobial activity of methanol and aqueous crude extracts of Lentinula edodes var. Koshin and Donko. Disk diffusion method was used to screen the antimicrobial activity and to assess the synergistic effect of the mushroom extracts. Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Phytochemical characterization of mushrooms extracts was achieved by analysis of total phenols, ortho-diphenols content and its antioxidant activity. The results noticed a positive relation between phenolic compounds content, antioxidant activity, and antimicrobial capacity of the mushroom's extracts. The L. edodes var. Koshin aqueous extracts, which contained the highest amount of total phenolic compounds, exhibited the highest scavenging capacity of ABTS which, in turns, exhibited the highest antimicrobial efficacy in inhibiting the growth of methicillin-resistant Staphylococcus aureus. Moreover, the combination between mushrooms extracts and commercial antibiotics showed favorable synergistic effects against tested bacteria. These results suggest that L. edodes var. Koshin may represent an important and valuable therapeutic source of compounds to be used against multidrug-resistant bacteria. |
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Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogensAntibiotic resistanceDonkoKlebsiella pneumoniaeKoshinShiitakeStaphylococcus aureusThe problematic increase in multidrug-resistant bacteria translates into the urgent need to discover novel and effective antimicrobial substances. Herein, mushrooms could be a promising alternative of natural source of new antimicrobials. The present work aimed to compare the phytochemical composition and antimicrobial activity of methanol and aqueous crude extracts of Lentinula edodes var. Koshin and Donko. Disk diffusion method was used to screen the antimicrobial activity and to assess the synergistic effect of the mushroom extracts. Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Phytochemical characterization of mushrooms extracts was achieved by analysis of total phenols, ortho-diphenols content and its antioxidant activity. The results noticed a positive relation between phenolic compounds content, antioxidant activity, and antimicrobial capacity of the mushroom's extracts. The L. edodes var. Koshin aqueous extracts, which contained the highest amount of total phenolic compounds, exhibited the highest scavenging capacity of ABTS which, in turns, exhibited the highest antimicrobial efficacy in inhibiting the growth of methicillin-resistant Staphylococcus aureus. Moreover, the combination between mushrooms extracts and commercial antibiotics showed favorable synergistic effects against tested bacteria. These results suggest that L. edodes var. Koshin may represent an important and valuable therapeutic source of compounds to be used against multidrug-resistant bacteria.This work was supported by the project I&T Companies in Co- Promotion FungiTech, Norte-01-0247-FEDER-033788; National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020 (CITAB-Center for the Research and Technology of Agro-Environmental and Biological Sciences), Centro de Química – Vila Real (UIDB/00616/2020) and UIDB/00690/2020 (CIMO-Centro de Investigaç˜ao de Montanha).Biblioteca Digital do IPBGarcia, JulianaAfonso, Ana CristinaFernandes, ConceiçãoNunes, Fernando M.Marques, GuilherminaSaavedra, Maria José2018-01-19T10:00:00Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/24287engGarcia, Juliana; Afonso, Ana; Fernandes, Conceição; Nunes, Fernando M.; Marques, Guilhermina; Saavedra, Maria José (2021). Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens. South African Journal of Chemical Engineering. ISSN 1026-9185. 35, p. 98-1061026-918510.1016/j.sajce.2020.09.008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:54:18Zoai:bibliotecadigital.ipb.pt:10198/24287Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:15:08.028314Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
title |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
spellingShingle |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens Garcia, Juliana Antibiotic resistance Donko Klebsiella pneumoniae Koshin Shiitake Staphylococcus aureus |
title_short |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
title_full |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
title_fullStr |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
title_full_unstemmed |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
title_sort |
Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens |
author |
Garcia, Juliana |
author_facet |
Garcia, Juliana Afonso, Ana Cristina Fernandes, Conceição Nunes, Fernando M. Marques, Guilhermina Saavedra, Maria José |
author_role |
author |
author2 |
Afonso, Ana Cristina Fernandes, Conceição Nunes, Fernando M. Marques, Guilhermina Saavedra, Maria José |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Garcia, Juliana Afonso, Ana Cristina Fernandes, Conceição Nunes, Fernando M. Marques, Guilhermina Saavedra, Maria José |
dc.subject.por.fl_str_mv |
Antibiotic resistance Donko Klebsiella pneumoniae Koshin Shiitake Staphylococcus aureus |
topic |
Antibiotic resistance Donko Klebsiella pneumoniae Koshin Shiitake Staphylococcus aureus |
description |
The problematic increase in multidrug-resistant bacteria translates into the urgent need to discover novel and effective antimicrobial substances. Herein, mushrooms could be a promising alternative of natural source of new antimicrobials. The present work aimed to compare the phytochemical composition and antimicrobial activity of methanol and aqueous crude extracts of Lentinula edodes var. Koshin and Donko. Disk diffusion method was used to screen the antimicrobial activity and to assess the synergistic effect of the mushroom extracts. Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Phytochemical characterization of mushrooms extracts was achieved by analysis of total phenols, ortho-diphenols content and its antioxidant activity. The results noticed a positive relation between phenolic compounds content, antioxidant activity, and antimicrobial capacity of the mushroom's extracts. The L. edodes var. Koshin aqueous extracts, which contained the highest amount of total phenolic compounds, exhibited the highest scavenging capacity of ABTS which, in turns, exhibited the highest antimicrobial efficacy in inhibiting the growth of methicillin-resistant Staphylococcus aureus. Moreover, the combination between mushrooms extracts and commercial antibiotics showed favorable synergistic effects against tested bacteria. These results suggest that L. edodes var. Koshin may represent an important and valuable therapeutic source of compounds to be used against multidrug-resistant bacteria. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/24287 |
url |
http://hdl.handle.net/10198/24287 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Garcia, Juliana; Afonso, Ana; Fernandes, Conceição; Nunes, Fernando M.; Marques, Guilhermina; Saavedra, Maria José (2021). Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens. South African Journal of Chemical Engineering. ISSN 1026-9185. 35, p. 98-106 1026-9185 10.1016/j.sajce.2020.09.008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135432858927104 |