The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets

Detalhes bibliográficos
Autor(a) principal: Ramos, Pablo Ivan Pereira
Data de Publicação: 2016
Outros Autores: Custódio, Márlon Grégori Flores, Quispe Saji, Guadalupe Del Rosario, Cardoso, Thiago, Silva, Gisele Lucchetti da, Braun, Graziela, Martins, Willames Marco Brasileiro da Silva, Girardello, Raquel, Vasconcelos, Ana Tereza Ribeiro de, Fernández, Elmer, Gales, Ana Cristina, Nicolás, Marisa Fabiana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1186/s12864-016-3070-y
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18085
Resumo: Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) supported this work through grants awarded to MFN (process no. E-26/110.315/2014) and ACG (process no. 2010/12891-9), respectively. The publication charges for this article were funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (process no. 23038.010041/2013-13)
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spelling Ramos, Pablo Ivan PereiraCustódio, Márlon Grégori FloresQuispe Saji, Guadalupe Del RosarioCardoso, ThiagoSilva, Gisele Lucchetti daBraun, GrazielaMartins, Willames Marco Brasileiro da SilvaGirardello, RaquelVasconcelos, Ana Tereza Ribeiro deFernández, ElmerGales, Ana CristinaNicolás, Marisa Fabiana2017-03-20T18:38:20Z2017-03-20T18:38:20Z2016RAMOS, P. I. P. et al. The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, v. 17, Suppl 8, p. 737, 2016.1471-2164https://www.arca.fiocruz.br/handle/icict/1808510.1186/s12864-016-3070-yFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) supported this work through grants awarded to MFN (process no. E-26/110.315/2014) and ACG (process no. 2010/12891-9), respectively. The publication charges for this article were funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (process no. 23038.010041/2013-13)Laboratório Nacional de Computação Científica. Petrópolis, RJ, Brasil / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilUniversidad Católica de Córdoba, CONICET. Facultad de Ingeniería. Córdoba, ArgentinaUniversidade Federal de São Paulo. Escola Paulista de Medicina. Laboratório Alerta. Department of Internal Medicine. Division of Infectious Diseases. São Paulo, SP, BrasilLaboratório Nacional de Computação Científica. Petrópolis, RJ, BrasilThe emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH), magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K. pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K. pneumoniae that could relate to surviving against the action of this antibiotic. Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.engBioMed CentralResistência aos antibióticosKlebsiella pneumoniaePatógenoPolimixina BRNA-seqAntibiotic resistanceKlebsiella pneumoniae,PathogenPolymyxin BRNA-seqTranscriptomicsThe polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targetsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18085/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRamos PIP The polymyxin.pdfRamos PIP The 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dc.title.pt_BR.fl_str_mv The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
spellingShingle The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
Ramos, Pablo Ivan Pereira
Resistência aos antibióticos
Klebsiella pneumoniae
Patógeno
Polimixina B
RNA-seq
Antibiotic resistance
Klebsiella pneumoniae,
Pathogen
Polymyxin B
RNA-seq
Transcriptomics
title_short The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_full The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_fullStr The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_full_unstemmed The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_sort The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
author Ramos, Pablo Ivan Pereira
author_facet Ramos, Pablo Ivan Pereira
Custódio, Márlon Grégori Flores
Quispe Saji, Guadalupe Del Rosario
Cardoso, Thiago
Silva, Gisele Lucchetti da
Braun, Graziela
Martins, Willames Marco Brasileiro da Silva
Girardello, Raquel
Vasconcelos, Ana Tereza Ribeiro de
Fernández, Elmer
Gales, Ana Cristina
Nicolás, Marisa Fabiana
author_role author
author2 Custódio, Márlon Grégori Flores
Quispe Saji, Guadalupe Del Rosario
Cardoso, Thiago
Silva, Gisele Lucchetti da
Braun, Graziela
Martins, Willames Marco Brasileiro da Silva
Girardello, Raquel
Vasconcelos, Ana Tereza Ribeiro de
Fernández, Elmer
Gales, Ana Cristina
Nicolás, Marisa Fabiana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos, Pablo Ivan Pereira
Custódio, Márlon Grégori Flores
Quispe Saji, Guadalupe Del Rosario
Cardoso, Thiago
Silva, Gisele Lucchetti da
Braun, Graziela
Martins, Willames Marco Brasileiro da Silva
Girardello, Raquel
Vasconcelos, Ana Tereza Ribeiro de
Fernández, Elmer
Gales, Ana Cristina
Nicolás, Marisa Fabiana
dc.subject.other.pt_BR.fl_str_mv Resistência aos antibióticos
Klebsiella pneumoniae
Patógeno
Polimixina B
RNA-seq
topic Resistência aos antibióticos
Klebsiella pneumoniae
Patógeno
Polimixina B
RNA-seq
Antibiotic resistance
Klebsiella pneumoniae,
Pathogen
Polymyxin B
RNA-seq
Transcriptomics
dc.subject.en.pt_BR.fl_str_mv Antibiotic resistance
Klebsiella pneumoniae,
Pathogen
Polymyxin B
RNA-seq
Transcriptomics
description Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) supported this work through grants awarded to MFN (process no. E-26/110.315/2014) and ACG (process no. 2010/12891-9), respectively. The publication charges for this article were funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (process no. 23038.010041/2013-13)
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-20T18:38:20Z
dc.date.available.fl_str_mv 2017-03-20T18:38:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, P. I. P. et al. The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, v. 17, Suppl 8, p. 737, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18085
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/s12864-016-3070-y
identifier_str_mv RAMOS, P. I. P. et al. The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, v. 17, Suppl 8, p. 737, 2016.
1471-2164
10.1186/s12864-016-3070-y
url https://www.arca.fiocruz.br/handle/icict/18085
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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