FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota

Detalhes bibliográficos
Autor(a) principal: Morais, J
Data de Publicação: 2019
Outros Autores: Marques, C, Teixeira, D, Durão, C, Faria, A, Brito, S, Cardoso, M, Macedo, I, Tomé, T, Calhau, C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.17/3997
Resumo: Background: Preterm infants are especially vulnerable to gut microbiota disruption and dysbiosis since their early gut microbiota is less abundant and diverse. Several factors may influence infants' gut microbiota, such as the mother's diet, mode of delivery, antibiotic exposure, and type of feeding. Objectives: This study aims to examine the factors associated with very-preterm neonate's intestinal microbiota, namely: (1) type of infant-feeding (breast milk, donor human milk with or without bovine protein-based fortifier, and preterm formula); (2) maternal diet; and (3) mode of delivery. Methods: This is an observational study conducted in a cohort of very preterm infants hospitalized in the neonatal intensive care unit of Maternidade Dr. Alfredo da Costa. After delivery, the mothers are asked to collect their own fecal samples and are invited to complete a semiquantitative food frequency questionnaire. The maternal diet will be classified in accordance to the Mediterranean Diet adherence score. Stool samples have been collected from very premature infants every 7 days for 21 days. DNA has been extracted from the fecal samples, and different bacterial genus and species will be quantified by real-time polymerase chain reaction. Results and conclusions: It is hypothesized that significant differences in the microbiota composition and clinical outcomes of very preterm infants will be observed depending on the type of infant feeding. In addition, this study will clarify how pasteurized donor's milk influences the intestinal microbiota colonization of preterm infants. This is a pioneer study developed in collaboration with the country's Human Milk Bank. We also expect to find microbiota alterations in infants according to the mode of delivery and to maternal diet. This study will contribute to increase the evidence on the effects of breast or donor human milk and its fortification with a bovine protein-based fortifier on infant microbiota.
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spelling FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut MicrobiotaMAC PEDMAC DIEClinical Trials as TopicDietFemaleHumansDelivery, ObstetricFeces / microbiologyFeeding Behavior*Gastrointestinal Microbiome*Infant Formula*Infant Nutritional Physiological PhenomenaInfant, Extremely PrematureInfant, NewbornInfant, Premature*Intensive Care Units, NeonatalLongitudinal StudiesMaternal Nutritional Physiological PhenomenaMilk, Human*Observational Studies as TopicSurveys and QuestionnairesBackground: Preterm infants are especially vulnerable to gut microbiota disruption and dysbiosis since their early gut microbiota is less abundant and diverse. Several factors may influence infants' gut microbiota, such as the mother's diet, mode of delivery, antibiotic exposure, and type of feeding. Objectives: This study aims to examine the factors associated with very-preterm neonate's intestinal microbiota, namely: (1) type of infant-feeding (breast milk, donor human milk with or without bovine protein-based fortifier, and preterm formula); (2) maternal diet; and (3) mode of delivery. Methods: This is an observational study conducted in a cohort of very preterm infants hospitalized in the neonatal intensive care unit of Maternidade Dr. Alfredo da Costa. After delivery, the mothers are asked to collect their own fecal samples and are invited to complete a semiquantitative food frequency questionnaire. The maternal diet will be classified in accordance to the Mediterranean Diet adherence score. Stool samples have been collected from very premature infants every 7 days for 21 days. DNA has been extracted from the fecal samples, and different bacterial genus and species will be quantified by real-time polymerase chain reaction. Results and conclusions: It is hypothesized that significant differences in the microbiota composition and clinical outcomes of very preterm infants will be observed depending on the type of infant feeding. In addition, this study will clarify how pasteurized donor's milk influences the intestinal microbiota colonization of preterm infants. This is a pioneer study developed in collaboration with the country's Human Milk Bank. We also expect to find microbiota alterations in infants according to the mode of delivery and to maternal diet. This study will contribute to increase the evidence on the effects of breast or donor human milk and its fortification with a bovine protein-based fortifier on infant microbiota.KargerRepositório do Centro Hospitalar Universitário de Lisboa Central, EPEMorais, JMarques, CTeixeira, DDurão, CFaria, ABrito, SCardoso, MMacedo, ITomé, TCalhau, C2022-03-10T15:42:48Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.17/3997engNeonatology. 2019;116(2):179-184.10.1159/000496547.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-10T09:44:53Zoai:repositorio.chlc.min-saude.pt:10400.17/3997Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:21:18.223652Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
title FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
spellingShingle FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
Morais, J
MAC PED
MAC DIE
Clinical Trials as Topic
Diet
Female
Humans
Delivery, Obstetric
Feces / microbiology
Feeding Behavior*
Gastrointestinal Microbiome*
Infant Formula*
Infant Nutritional Physiological Phenomena
Infant, Extremely Premature
Infant, Newborn
Infant, Premature*
Intensive Care Units, Neonatal
Longitudinal Studies
Maternal Nutritional Physiological Phenomena
Milk, Human*
Observational Studies as Topic
Surveys and Questionnaires
title_short FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
title_full FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
title_fullStr FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
title_full_unstemmed FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
title_sort FEEDMI: A Study Protocol to Determine the Influence of Infant-Feeding on Very-Preterm-Infant's Gut Microbiota
author Morais, J
author_facet Morais, J
Marques, C
Teixeira, D
Durão, C
Faria, A
Brito, S
Cardoso, M
Macedo, I
Tomé, T
Calhau, C
author_role author
author2 Marques, C
Teixeira, D
Durão, C
Faria, A
Brito, S
Cardoso, M
Macedo, I
Tomé, T
Calhau, C
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório do Centro Hospitalar Universitário de Lisboa Central, EPE
dc.contributor.author.fl_str_mv Morais, J
Marques, C
Teixeira, D
Durão, C
Faria, A
Brito, S
Cardoso, M
Macedo, I
Tomé, T
Calhau, C
dc.subject.por.fl_str_mv MAC PED
MAC DIE
Clinical Trials as Topic
Diet
Female
Humans
Delivery, Obstetric
Feces / microbiology
Feeding Behavior*
Gastrointestinal Microbiome*
Infant Formula*
Infant Nutritional Physiological Phenomena
Infant, Extremely Premature
Infant, Newborn
Infant, Premature*
Intensive Care Units, Neonatal
Longitudinal Studies
Maternal Nutritional Physiological Phenomena
Milk, Human*
Observational Studies as Topic
Surveys and Questionnaires
topic MAC PED
MAC DIE
Clinical Trials as Topic
Diet
Female
Humans
Delivery, Obstetric
Feces / microbiology
Feeding Behavior*
Gastrointestinal Microbiome*
Infant Formula*
Infant Nutritional Physiological Phenomena
Infant, Extremely Premature
Infant, Newborn
Infant, Premature*
Intensive Care Units, Neonatal
Longitudinal Studies
Maternal Nutritional Physiological Phenomena
Milk, Human*
Observational Studies as Topic
Surveys and Questionnaires
description Background: Preterm infants are especially vulnerable to gut microbiota disruption and dysbiosis since their early gut microbiota is less abundant and diverse. Several factors may influence infants' gut microbiota, such as the mother's diet, mode of delivery, antibiotic exposure, and type of feeding. Objectives: This study aims to examine the factors associated with very-preterm neonate's intestinal microbiota, namely: (1) type of infant-feeding (breast milk, donor human milk with or without bovine protein-based fortifier, and preterm formula); (2) maternal diet; and (3) mode of delivery. Methods: This is an observational study conducted in a cohort of very preterm infants hospitalized in the neonatal intensive care unit of Maternidade Dr. Alfredo da Costa. After delivery, the mothers are asked to collect their own fecal samples and are invited to complete a semiquantitative food frequency questionnaire. The maternal diet will be classified in accordance to the Mediterranean Diet adherence score. Stool samples have been collected from very premature infants every 7 days for 21 days. DNA has been extracted from the fecal samples, and different bacterial genus and species will be quantified by real-time polymerase chain reaction. Results and conclusions: It is hypothesized that significant differences in the microbiota composition and clinical outcomes of very preterm infants will be observed depending on the type of infant feeding. In addition, this study will clarify how pasteurized donor's milk influences the intestinal microbiota colonization of preterm infants. This is a pioneer study developed in collaboration with the country's Human Milk Bank. We also expect to find microbiota alterations in infants according to the mode of delivery and to maternal diet. This study will contribute to increase the evidence on the effects of breast or donor human milk and its fortification with a bovine protein-based fortifier on infant microbiota.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2022-03-10T15:42:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.17/3997
url http://hdl.handle.net/10400.17/3997
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neonatology. 2019;116(2):179-184.
10.1159/000496547.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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